Sentences with phrase «of genes of interest»

The Tol2 Gateway - Compatible Toolbox is based on the original Tol2kit generated by the Chi - Bin Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission spectra (N - and C - terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of interest.

Botstein and Davis» landmark conceptual breakthrough, published in 1980, gave researchers the tools to trace and map out the genetic inheritance of disease in humans, while Hogness» work provided the means of identifying the precise physical location of genes of interest on chromosomes.

«We have deposited all the data and the toolkit in a public platform, which will help researchers identify and validate the functions of their genes of interest,» says Auwerx, placing the project within the context of EPFL's ongoing efforts to advance open science.

«We therefore looked for patient characteristics that would account for this result; next generation sequencing (NGS) of the genes of interest was performed on the archival tissue samples from the patients» primary tumours,» explained Ciardiello.

Most tests require three groups: controls, genetically altered mice with one copy of the gene of interest, and genetically altered mice with two copies of that gene.

A well - defined cell type that has been widely used to identify the consequences of ablation or ectopic expression of a gene of interest.

One way to avoid the pitfalls of overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of interest with a fluorescent protein fusion.

If there are no negative effects on plant regeneration as a result of either expression or knockout of the gene of interest, and the genotype of interest, then the expected recovery per experiment is five to ten independent transgenic lines.

cassette upstream of a gene of interest blocks transcription when Cre is absent.

Enormously diverse libraries of a gene of interest are continuously generated making the system attractive for large scale multifaceted screening.

These procedures generate animals that carry specific genetic alterations, and recreate the underlying cause of a human disorder or uncover the role of any gene of interest.

Not exact matches

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There are a wide range of reproductive strategies resulting from the competing interests at various levels of selection, that is, the level of individual genes / gene clusters, the level of the individual and the level of the population / species.

And the same is going to be true now as public interest shifts from nuclear physics to molecular biology, from the splitting of atoms to the splicing of genes.

It is, as well, an antidote to Mississippi Burning, a dishonest, award - winning new film in which blacks wait patiently and fearfully in the background for deliverance by two white FBI agents, played by Gene Hackman and Willem Dafoe, who zealously bend the law in the interest of justice — a film one fears will have a profound effect on the way many Americans view their nation in the King Years («The Dream Dafoed,» as the Village Voice put it).

Wilson's interest for us, then, may lie in the fact that he articulates, at least in the «disenchanted» and secularistic tenor of his thought, what many educated people of our society increasingly suspect to be the case: matter and genes really are all that there is or ever will be.

Evolution was not of major interest to most of these biologists, but insofar as they had a theory of it, it was a theory in terms of mutations of individual genes, carried by individual organisms and submitted to natural selection.

When asked why we should take their ideas seriously if they are no more than the predetermined products of genes or conditioning, they are inclined to respond, «It is an interesting paradox, isn't it?»

My relatively muted response to the refined starches makes me interested in learning how many copies of the AMY1 «carb breakdown» gene I have.

«IRRI's Green and Gene Revolution failed because they do not serve the interests of the farmers; rather it served to perpetuate the systemic exploitation and oppression of the toiling masses,» said Flores.

During my time, more tools became available for plant breeders to transfer genes of interest and improve selection efficiency.

As a rule of thumb, plant breeders in both public research institutes and private companies use genetic engineering only in the following situations: 1) the gene of interest is not found within the crop gene pool, 2) if found in the gene pool, transfer will be difficult due to incompatibility and other breeding problems or 3) the transfer of the gene will take a long time as in the case of perennial crops like coconut.

With consumer appetites shifting towards more «natural» offerings, there is renewed commercial interest in new non-GMO breeding techniques — such as gene editing — which have the potential to give rise to a range of products with enhanced quality and novel attributes.

It greatly increases the accuracy of incorporating only the gene of interest, and its associated trait, into a new rice variety.

Using genetic modification in this way can improve the accuracy of identifying genes of interest and speed up the breeding process, even though the end - product is not GM rice.

«We're getting a steady flow of calls on the main complaints,» said Gene Russianoff of the New York Public Interest Research Group, which runs a voter help hotline.

LCV Action Fund President Gene Karpinski praised Bishop's support of clean energy legislation, saying he «stands with the people of New York who need clean energy jobs now, and not with the Big Oil companies and special interests who are blocking progress in Washington.»

There has been considerable interest, for example, in a gene that produces low levels of an enzyme called monoamine oxidase A (MAOA) in the brain.

Sebat's team was especially interested in the parts of noncoding DNA that regulate gene expression.

Because this imprinting affects hundreds of genes that are non-coding, including microRNAs and non-coding RNAs, it's a very interesting fine - tuning mechanism for the dosage of gene expression in the brain and elsewhere in the body.»

When looking for genes that might play important roles in the metabolism of healthy and cancerous liver cells, Wang and his colleagues became interested in a gene called SLC13A5, which produces a protein that transports citrate into cells.

But they do seem to have some interest in the opposite gender: Sometimes these parthenogens are mate with males of different species, creating a species with 50 percent more genes than normal.

As well as understanding the implications of finding this new variant of the botulism gene cluster in a non-clostridial species of gut bacterium, the team are interested in exploring how it might help in developing new treatments for diseases.

For a male fathering children with many mates, it's in the interest of his genes that the baby suck as much out of the mother as it can.

Women with precancer or cancer were most likely to have particular versions of the viral gene E7, which researchers are now interested in targeting.

If a biomolecule of interest were tagged with the GFP gene, Prasher thought, a fluorescent signal would show when and where in the organism that gene or the protein it created was being put to use.

The gene, called NPC1L1, is of interest because it is the target of the drug ezetimibe, often prescribed to lower cholesterol.

Another way to pass on the seemingly nonreproducing gay gene would be for a nonreproducing gay man to have an extra interest in seeing that the offspring of his siblings survive.

Interested in the history of lentiviruses — the group of retroviruses to which HIV and its simian (monkey) relatives, the SIVs belong — Welkin Johnson, from Boston College, USA, and colleagues focused on an antiviral gene called TRIM5.

Their interest was in understanding the functional importance of genes inherited from archaic humans more broadly.

«What we decided to do was take a completely unbiased approach where instead of targeting individual genes of interest, we would express randomized guides inside of the cell,» Lu says.

The last gene of interest was TGM5, which encodes a protein that stabilizes protein groups.

Viral - mediated gene transfer now enables investigators to overexpress or knock out a gene of interest within highly localized regions of brain.

There are probably genes that code for being a nice guy, being a son - of - a-bitch; it's not a one - to - one relationship, but there are probably things that enhance aggressivity — well, things that enhance sociability — so that's interesting to know.

Although the regulation of this gene has a strong «cell cycle control» component, I was more interested in studying cell cycle regulation «proper.»

He is interested in reactivating dormant genes or changing the regulation of active genes in embryos to bring back ancestral traits that have been lost in evolution.

«The interesting thing is that when we looked the same dog genes in human breast cancer, epigenetic aberrations occur in the same regions of DNA.

In the intervening years, we have come to realize that many of the most interesting and important phenomena in human biology are not caused by any single gene.

He has been able to piggyback on previous work on the development of the chick embryo: When researchers interested in some process use a stain to show gene activity, the stain affects the whole embryo, providing clues about which genes are active in tail growth.

«The challenge has been how to direct certain therapies designed to manipulate genes of interest in specific cells without developing a specific drug carrier for each specific cell type.