D, Confocal Z - projections
of geniculate ganglion from 5 - HT3AGFP mice 5 d after FluoroGold injection showing expression of GFP (green) and FluoroGold label (FG; magenta).
The majority of cells
of the geniculate ganglion are gustatory, contributing to the chorda tympani and greater superficial petrosal nerves (Foley and DuBois, 1943; van Buskirk, 1945) innervating, respectively, fungiform papillae and palatal taste fields.
GFP immunoreactivity was detected in a subset
of geniculate ganglion neurons (169/682, 24.8 %; Fig. 2B, C), whereas the P2X3 signal was present in nearly all ganglion cells (664/682, 97.4 %; Fig. 2B, C).
Interestingly, when nonbarbiturate anesthetics are used, a concentration - dependent increase in sideband excitation is observed in a subset
of geniculate ganglion neurons (Wu et al., 2015).
Somatosensory innervation of these taste fields arises from the trigeminal nerve; therefore, cells
of the geniculate ganglion that innervate taste fields are gustatory rather than somatosensory in function.
Our data demonstrate that a subset
of geniculate ganglion neurons expresses 5 - HT3 receptors and innervates taste buds, including serotonergic type III cells.
A, Confocal Z - projection of 10 0.7 μm optical slices
of geniculate ganglion from a 5 - HT3AGFP mouse showing expression of GFP (green) and P2X3 (magenta).
Not exact matches
It now looks like they have their lateral
geniculate nucleus (LGN)-- part
of the thalamus in the middle
of the brain — to thank for this «blindsight».
Searching for answers, Shatz decided to focus on the lateral
geniculate nucleus (LGN), a clump
of tissue shaped like a piece
of elbow macaroni, set behind each eyeball, that serves as a relay station shunting visual signals to the ocular dominance columns.
When they tracked the cells» axons from the eye into the brain, they saw that many
of them terminated in the lateral
geniculate nucleus (LGN), the first relay station in the brain for visual information.
Specifically, they have found evidence
of processing in the human lateral
geniculate nucleus (LGN), a small node in the thalamus in the middle
of the brain that relays nerve impulses from the retina to the primary visual cortex.
This image shows the localization
of the human lateral
geniculate nucleus, using high - resolution functional MRI (orange) and anatomical MRI (green).
In both primates and rodents, messages from the two eyes enters the brain through a small structure called the lateral
geniculate nucleus or LGN, which is made
of slivers
of nerve cells, arranged like sponge in a layer cake.
Approximately 25 %
of all WT
geniculate neurons (5/19; Fig. 5) responded to exogenous 5 - HT with an increase in intracellular calcium, whereas no neurons from 5 - HT3AKO mice responded (0/25, p = 0.011, Fisher's exact test; Fig. 5).
A, RT - PCR showing the presence
of 5 - HT3A transcripts in brain and
geniculate ganglion
of WT mice, but the lack
of 5 - HT3A mRNA in
geniculate ganglion
of 5 - HT3AKO mice.
Z - stack images
of taste buds and
geniculate ganglia were collected on an Olympus Fluoview FV300 laser scanning confocal microscope with a 60 × oil - immersion objective [numerical aperture (NA) 1.3] and 20 × oil - immersion objective (NA 0.7) or on a Leica TCS SP5 laser scanning confocal microscope with a 63 × oil - immersion objective (NA 1.4).
In mice expressing GFP under the control
of the 5 - HT3A promoter, a subset
of cells in the
geniculate ganglion and nerve fibers in taste buds are GFP - positive.
We next tested the responsiveness
of GFP - expressing and GFP - nonexpressing
geniculate ganglion neurons to exogenously applied 5 - HT.
In situ hybridization on sections
of gustatory ganglia (
geniculate, petrosal / nodose) and a somatosensory ganglion (trigeminal) were performed to reveal serotonin receptor gene expression at the cellular level.
RT - PCR and in situ hybridization confirmed the presence
of 5 - HT3A mRNA in the
geniculate ganglion.
RNA was extracted from
geniculate ganglia
of 5 - HT3AKO and WT mice (3 mice each) according to manufacturer's instructions using the RNeasy Micro kit (Qiagen), including a 30 min DNase I treatment at room temperature for removal
of genomic DNA.
Normalized responses
of Fura -2-AM loaded
geniculate ganglion neurons revealed that a subset (solid circles; 5/19)
of WT neurons were activated by 10 μm 5 - HT; no neurons from 5 - HT3AKO animals (open circles; 0/25) responded to 5 - HT, yet all responded to 10 μm ATP and 55 mm KCl.
Biophysical properties and responses to glutamate receptor agonists
of identified subpopulations
of rat
geniculate ganglion neurons
In addition, we tested whether other 5 - HT receptors were upregulated after genetic deletion
of 5 - HT3 by measuring 5 - HT responses in isolated
geniculate ganglion neurons
of WT and 5 - HT3AKO mice.
The anterograde (forward) tracing results
of retinal projections in the garden warbler demonstrated virtually identical connections between the eye and the brain as known from other lateral - eyed bird species [e.g. 15]--[18]: fibers either projected onto the contralateral optic tectum (part
of the tectofugal system), to the nucleus
of the basal optic root (part
of the accessory pathway)(data not shown) or curved into the thalamus innervating the dorsal
geniculate complex (Gld, for details, see Fig. 1C, 2A, D).