Sentences with phrase «of human adenovirus»

Partial block to transcription of human adenovirus type 2 late genes in abortively infected monkey cells

Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells

Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells

Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells

Posttranscriptional block to synthesis of a human adenovirus capsid protein in abortively infected monkey cells

Humans are infected by common serotypes of human adenovirus such as AdHu5 and a significant percentage has neutralizing antibodies to this serotypes, which impair the efficacy of AdHu5 based vaccines.

These viruses do not circulate in the human population and fail to carry neutralizing B cell epitopes that cross-react with the common serotypes of human adenoviruses.

Not exact matches

There are innumerable different viruses, but the human adenovirus 5, which normally causes the symptoms of a typical cold, has substantial advantages: Its genome can be replaced completely by an artificial one which contains only «useful» genes.

«No data from humans vaccinated with adenovirus 5 vectors are described in this paper,» says one of them, Dan Barouch of the Beth Israel Deaconess Medical Center at Harvard Medical School.

But in mice that had been exposed to human adenovirus, only the chimp virus - based vaccine worked, the researchers report in the March issue of the Journal of Virology.

A possible problem facing a human vaccine is that some people already have an immune response to some strains of adenoviruses, which would reduce the body's response to the vaccine strain.

Now, a new report confirms that the Davis outbreak was the first known case of an adenovirus jumping from monkeys to humans.

To assess cross-neutralization of TMAdV by known human adenoviruses, 7 pools of in - house reference sera at the VRDL (rabbit hyperimmune sera) and collectively containing antibodies to human adenovirus serotypes 1 through 35 were available for testing.

The discovery of TMAdV, a novel adenovirus with the capacity to infect both monkeys and humans, suggests that adenoviruses should be monitored closely as potential causes of cross-species outbreaks.

The significant overall sequence divergence of TMAdV from known human and simian adenoviruses is highlighted by the finding that PAdV - A (porcine adenovirus A), a non - primate mammalian adenovirus, shared only a slightly less similar whole - genome pairwise identity to TMAdV of 47.0 % (Fig. 4).

However, the high sequence divergence in the fiber protein (Table 2), as well as the absence of fiber motifs conserved among adenoviruses that bind CAR [36], [37](coxsackievirus - adenovirus receptor) or CD46 [38], [39], [40](data not shown), suggest that neither of these two human adenoviral receptors may be the attachment receptor for TMAdV.

The closest human adenoviral relatives were the species D adenoviruses, which share 54.3 % to 55.1 % identity to TMAdV, with human adenoviruses of other species slightly less similar (51.1 % — 54.6 %).

We used the Virochip, a microarray designed to detect all viruses, to identify a new species of adenovirus (TMAdV, or titi monkey adenovirus) that caused a deadly outbreak in a colony of New World titi monkeys at the California National Primate Research Center (CNPRC), and also infected a human researcher.

Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans.

After removal of similar viral genomes, bootscan plots of the whole genome and individual genes from a subset representing human / simian adenoviruses in species A — G and all non-primate vertebrate adenoviruses were generated.

Human adenoviruses do not usually replicate in monkey cells in the absence of helper viruses [11], and do not productively infect rodents (and vice versa)[12].

Some serotypes, such as human adenovirus type 14 (HAdV - 14), have been associated with severe and potentially fatal outbreaks of pneumonia in residential facilities and military bases [4].

Adenovirus PCR was performed on a TMAdV - positive clinical sample, a TMAdV culture, and a human adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify TAdenovirus PCR was performed on a TMAdV - positive clinical sample, a TMAdV culture, and a human adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify Tadenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify TMAdV [27].

Second, our results show that PCR assays for human adenoviruses in common use are capable of detecting TMAdV.

In this regard, the high sequence divergence of TMAdV relative to the known human / simian adenoviruses (Fig. 3), and comparable sequence similarity in the polymerase gene to a porcine adenovirus (Figs. 3 and S1) are striking.

Although sequencing of PCR amplicons for human adenoviruses is not performed routinely in diagnostic virology, TMAdV would presumably have been detected previously in large - scale studies of hexon sequencing of Ad field isolates if it were circulating in the community [46], [47].

The decreased levels of neutralizing Abs to TMAdV in the researcher (1 ∶ 32) and a family member (1 ∶ 8) relative to those in infected titi monkeys (up to > 1 ∶ 512) are consistent with a recent study showing much higher levels of neutralizing antibodies in chimpanzees than in humans with adenovirus infections, possibly due to more robust adenovirus - specific T - cell responses in humans than in monkeys [45].

Respiratory viral testing failed to detect evidence of respiratory syncytial virus, adenovirus, influenza virus A and B, human metapneumovirus, and parainfluenza virus types 1, 2, and 3.

The scanning nucleotide pairwise identities of TMAdV relative to representative human (yellow) or simian (brown) adenoviruses in species A — G, porcine adenovirus (red), and fowl adenovirus (green) are shown.

Open Reading Frame E3 - 10.9 K of Subspecies B1 Human Adenoviruses Encodes a Family of Late Orthologous Proteins That Vary in Their Predicted Structural Features and Subcellular Localization.

Such antibodies are expected to reduce the efficacy of vaccines based on common human serotypes of adenovirus in a human target population.

To do this, a team led by Matthew Weitzman of the University of Pennsylvania crosslinked the host factors of infected human cells with the DNA of adenovirus, herpes simplex virus and vaccinia virus.

Characterization of the translational defect to fiber synthesis in monkey cells abortively infected with human adenovirus: Role of ancillary leaders

Scale - Up of the Adenovirus Expression System for the Production of Recombinant Protein in Human 293S Cells.

Increased permissivity of monkey cells to human adenovirus multiplication is affected by culturing conditions and correlates with both synthesis of virion fiber protein and altered splicing of its mRNA

Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a subpopulation of cells that experessed human IgG, as shown by the increased fluorescence intensity.

Additional experiments using RNA isolated from stocks of viruses from different virus families showed no cross reactivity of the assay with influenza A virus, influenza B virus, rhinovirus, human coronaviruses 229E, OC43, and NL63, and adenovirus (data not shown).

It contains highly attenuated strains of CDV, human measles virus, canine adenovirus - 2 (CAV - 2) and canine parainfluenza virus (CPiV).

Improving the oncolytic potency of agents has been hampered by the inability to study host - vector interactions in immune - competent systems, since human serotype adenoviruses do not productively replicate in animal tissues.