Inhibition of growth, production of insulin - like growth factor - II (IGFII), and expression of IGF - II mRNA
of human cancer cell lines by antagonistic analogs of growth hormone - releasing hormone in vitro.
NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number
of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.
«We've seen this in six different types
of human cancer cell lines in the lab,» Choudhury said.
Not exact matches
Introducing
human prostate
cancer cell lines into mice, Wu and his colleagues saw a particular enzyme called MAOA activate a cascade
of signals that made it easier for tumor
cells to invade and grow in bone.
«This model was trained on genetic data from
human tumors in The
Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greene
Cancer Genome Atlas and was able to predict response to certain inhibitors that affect
cancers with overactive Ras signaling in an encyclopedia
of cancer cell lines,» Greene
cancer cell lines,» Greene said.
Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role
of HIF - 1 in
cancer, and to demonstrate the potential for inhibiting this protein in
cancer therapy, we engineered a
human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.
The researchers observed the effect
of the synthetically produced molecule, JK - 31, on the growth and proliferation
of a model
human breast
cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process
of the division
of cancer cells, and therefore inhibited the proliferation
of the
cells.
During lab testing, the compound «markedly inhibited» the growth
of three
human pancreatic
cancer cell lines five days after treatment and induced the death
of pancreatic
cancer cells.
They found out that TiY is capable
of distinguishing TICs from non-TICs in various
human lung
cancer cell lines and patient - derived lung tumors.
Beyond lung
cancer, TiY is able to target TICs in 28 types
of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate
cancer.
Using both fruit fly and
human lung
cancer cell lines, researchers targeted two
of the most common genetic mutations associated with NSCLC — Ras and PTEN (P13K).
To see whether
cancer stem
cell renewal involves a chain
of events similar to that used by embryonic stem
cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two
human breast
cancer cell lines that responded to low oxygen by ramping up production
of the protein ALKBH5, which removes methyl groups from mRNAs.
Tests with kidney
cancer and two
human uterine sarcoma
cell lines, one with multidrug resistance, showed that 10
of these new compounds were impressively potent against all three
cell lines, the researchers reported.
Through these effects, the PERY peptide reduced the proliferation
of several (but not all)
cancer cell lines in culture and inhibited the growth
of a
human breast
cancer xenograft in mice.
In earlier studies involving animal models and
human cancer cell lines, researchers found that breast
cancer spreads when three specific
cells are in direct contact: an endothelial
cell (a type
of cell that
lines the blood vessels), a perivascular macrophage (a type
of immune
cell found near blood vessels), and a tumor
cell that produces high levels
of Mena, a protein that enhances a
cancer cell's ability to spread.
The researchers showed that cabazitaxel worked better than docetaxel in
human prostate
cancer cells lines that were resistant hormone treatment, both in terms
of slowing
cancer -
cell growth and in its ability to kill
cancer cells.
A team
of researchers in Germany and Denmark led by Steven Johnsen, Professor at the University Medical Center Göttingen, Germany, used
human prostate
cancer cell lines and depleted them
of the DNA - binding protein CHD1.
The resulting «map»
of gene - drug interactions allowed the researchers to accurately predict the responses
of multiple
human cancer cell lines to different chemotherapy agents based on the
cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response
of breast and ovarian tumor
cells to common classes
of chemotherapy treatment.
He is researching the functions and properties
of human sulfotransferase (EST) enzymes in
human breast
cancer cell lines.
To test their hypothesis, the researchers carried out experiments on
human melanoma
cells, a
line of cancer cells they chose for their ability to grow easily and quickly.
Slashing the amount
of HSF1 in several
lines of human cancer cells diminished growth and survival.
In studies
of laboratory - grown
human tumor
cell lines, the drug disrupted tumor
cell division and prevented growth
of advanced
cancer cells.
It demonstrates that alterations in these
cells lines do indeed match changes in samples
of human bladder
cancer.
In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination
of drug inhibitors halted colorectal
cancer growth more effectively in both mouse models and
human colorectal
cancer cell lines.
The researchers tested honokiol on
cell lines derived from
human cancers of the oral cavity, larynx, tongue, and pharynx.
The goal
of the first experiment was to see whether lncRNAs are differentially expressed in prostate
cancer by measuring total RNA from prostate
cancer cell lines and normal epithelial prostatic
cells using NCode
human ncRNA array and SurePrint G3
human lncRNA microarrays.
Dr. Bauman and her colleagues treated
human head and neck
cancer cells in the laboratory with varying doses
of sulforaphane and a control, and compared them to normal, healthy
cells that
line the throat and mouth.
For this reason, the National
Cancer Institute maintains a library of 60 authenticated human cancer cell lines for the purposes of research, called the NCI
Cancer Institute maintains a library
of 60 authenticated
human cancer cell lines for the purposes of research, called the NCI
cancer cell lines for the purposes
of research, called the NCI - 60.
McKay and Tsai searched
human cancer cell lines and found a
human version
of nucleostemin.
But a computer game that puts those kids on the front
line of a battlefield inside the
human body — where
cancer cells and chemotherapy drugs are slogging it out for supremacy — just might persuade them that it's important to keep taking the drugs.
For the time course study,
cells were treated with 20 μM
of EGCG for 12, 24, 48, 72, or 144 h.
Human colon
cancer cell line HT - 29 and prostate
cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.
To determine whether the reactivation
of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other
cell lines, we studied the effects
of EGCG treatment on the methylation status and mRNA levels
of p16INK4a or RARβ in three other
human cancer cell lines (Fig. 3C) ⇓.
Interestingly, two recent reports that have catalogued recurrent focal deletions in large cohorts
of human tumors and
cancer cell lines.
The authors studied a standard panel
of 60 established
human tumor
cell lines representing nine different
human cancers, as well as several specimens
of human primary ovarian
cancer.
Decreased functional expression
of Grp78 and Grp94 inhibits proliferation and attenuates apoptosis in a
human gastric
cancer cell line in vitro.
Significantly increased expression
of OCT4 and ABCG2 in spheroid body - forming
cells of the
human gastric
cancer MKN - 45
cell line.
They fused mouse B
cells — antibody - producing
cells of the immune system — with
human myeloma (also known as B
cell cancer)
cell lines in a new technique called hybridoma technology.
Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched subpopulation could be isolated by exposing
human metastatic breast
cancer cell lines to cycles
of chronic hypoxia followed by reoxygenation.
To study the effect
of hypoxia / reoxygenation cycles on breast
cancer, we exposed two metastatic
human breast
cancer cell lines (MDA - MB 231 and BCM2) to cycles
of chronic hypoxia and nutrient deprivation.
HuH - 7 (
human HCC), HepG2 (
human hepatocellular blastoma), 293T (
human embryonic kidney fibroblast), HeLa (
human cervical
cancer cells), NIH 3T3 (murine fibroblast), and HCT116 (human colon cancer) and its derived cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD
cancer cells), NIH 3T3 (murine fibroblast), and HCT116 (
human colon
cancer) and its derived cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD
cancer) and its derived
cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive
Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD
Cancer Center
of the Johns Hopkins University School
of Medicine, Baltimore, MD; ref.
«Basically, this study shows that the genetic makeup
of individual
human embryonic stem
cell lines is unique in the numbers
of copies
of certain genes that may control traits and things like disease susceptibility,» said Teitell, who also is an associate professor
of pathology and laboratory medicine and a researcher at UCLA's Jonsson Comprehensive
Cancer Center.
I wanted to inactivate the gene BRAF (a kinase implicated in several
human cancers) in A549
cells (a
human lung
cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections
of scientific articles (gasp).
The goal was to deepen the knowledge about the pharmacological and cytotoxic effects
of the drug Temodex in vitro in different
human brain
cancer cell lines.
(a kinase implicated in several
human cancers) in A549
cells (a
human lung
cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections
of scientific articles (gasp).
In a series
of lab experiments with
cell lines,
human xenograft tumors in mice and primary
human prostate
cancer samples, the researchers demonstrated that miR - 34a inhibits prostate
cancer stem
cells by suppressing CD44.
canSAR contains information on the entire
human proteome — the protein equivalent
of the
human genome - as well as linking to information on more than 1,000
cancer cells lines, used by researchers to study the effect
of new drugs in the lab.
Cultures
of the
human triple negative breast
cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.
On the basis
of studies on the XMRV - producing
human prostate
cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mice.
Important features
of XMRV biology include (1) tropism for a variety
of cell lines, including prostate
cancer DU145 and LNCaP
cells [27], [43], [48], and
human neural
cell types [57], (2) adaptations that promote growth in prostate epithelium and
human - derived prostate
cancer cell lines including an androgen response element in the promoter region [58] and downregulation
of APOBEC3G [59], and (3) cellular effects with potential oncogenic properties including increased tumor aggressiveness mediated by downregulation
of p27 [60] and differential regulation
of several microRNAs [61].
In 2011, Paprotka, et al. reported that XMRV likely originated through recombination between 2 endogenous murine retroviruses, PreXMRV - 1 and PreXMRV - 2, during in vivo passaging
of the
human prostate
cancer xenograft CWR - R1, resulting in establishment
of the XMRV - infected 22Rv1
cell line [38].