The in vitro activity and specificity
of human endothelial cell - specific promoters in porcine cells.
We also demonstrate that NS1 from DENV1, DENV2, DENV3, and DENV4 triggers endothelial barrier dysfunction, causing increased permeability
of human endothelial cell monolayers in vitro.
To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer
of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.
Palmitate and oleate have distinct effects on the inflammatory phenotype
of human endothelial cells.
To understand this phenomenon better, the regenerative properties
of human endothelial cells have been studied extensively [1,4 - 6].
Not exact matches
So Daniel Anderson at the Massachusetts Institute
of Technology exposed
human bone marrow stem
cells to biodegradable nanoparticles carrying the
human gene for vascular
endothelial growth factor (VEGF), which attracts blood vessels to injury sites.
In experiments conducted on
human lung
endothelial cells and in mice, the researchers showed that NS1 caused permeability
of the endothelium, which lines the walls
of blood and lymph vessels.
In a previous related study published in the Journal
of Materials Science: Materials in Medicine, the same team
of NTU scientists found that fish scale - derived collagen would induce
human umbilical vein
endothelial cells to express 2.5 times more
of a specific type
of collagen responsible for blood vessel formation, as compared to
endothelial cells cultured on bovine collagen.
Specifically, the study — reported online in The Journal
of Infectious Diseases — shows that E. coli K1 modulates the protein peroxisome proliferator - activated receptor - gamma (PPAR - γ) and glucose transporter - 1 (GLUT - 1) levels at the blood - brain barrier in
human brain microvascular
endothelial cells.
In earlier studies involving animal models and
human cancer
cell lines, researchers found that breast cancer spreads when three specific
cells are in direct contact: an
endothelial cell (a type
of cell that lines the blood vessels), a perivascular macrophage (a type
of immune
cell found near blood vessels), and a tumor
cell that produces high levels
of Mena, a protein that enhances a cancer
cell's ability to spread.
The researchers used the power
of gene sequencing and clever computational methods to uncover the «source code» for
human endothelial cells and learn how that code is disturbed in
human disease.
In the chamber, tubes about the thickness
of a
human hair were lined with
endothelial cells as in natural blood vessels.
Professor Takao Hamakubo's group at the Department
of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology (RCAST), have shown that PTX3 forms strong bonds with histones and partially unfolds, leading to a disordered coaggregation
of histone and PTX3 and protecting
human endothelial cells from damage.
Malinski's team has developed unique methods and systems
of measurements using nanosensors, which are about 1,000 times smaller in diameter than a
human hair, to track the impacts
of Vitamin D3 on single
endothelial cells, a vital regulatory component
of the cardiovascular system.
The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing
human intestinal microvascular
endothelial cells on the opposite side
of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.
In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which
cells from a
human intestinal
cell line originally isolated from a tumor were cultured in one
of two parallel running channels, separated by a porous matrix - coated membrane from
human blood vessel - derived
endothelial cells in the adjacent channel.
He then injected
human endothelial cells into the collagen structures
of blood vessels to recolonise the surfaces
of blood vessels.
In
human endothelial cell monolayers in culture, NS1 from any
of the four DENV serotypes triggered
endothelial barrier permeability.
Related to this, the discovery
of an increased
endothelial cell population in the periphery
of the
human cornea has prompted an investigation for evidence
of the existence
of stem - like
cells in the
endothelial periphery.
The present demonstration
of T
cell - mediated apoptosis
of allogeneic corneal
cells from CD4 KO mice is consistent with previous findings, which noted the presence
of apoptotic keratocytes and corneal
endothelial cells in rejected corneal allografts in
humans and rats respectively (5, 32).
Blake DA, Yu H, Young DL, Caldwell DR. Matrix stimulates the proliferation
of human corneal
endothelial cells in culture.
In keeping with this, recent studies showed that flavanols, a subclass
of flavonoids that is richly represented in natural cocoa beans, increase NO production by cultured
human vascular
endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries
of healthy
humans.
Figure 2 shows sets
of results for four areas
of human corneal
endothelial tissue; central endothelium (defined by
endothelial cells within a 4 mm trephined boundary), central - intermediate endothelium (defined by
endothelial cells within an 8 mm trephined boundary), intermediate - peripheral endothelium (defined by
endothelial cells between the 4 mm trephine edge and 12 mm from the center
of the corneal endothelium), and the peripheral endothelium (defined by the 8 mm trephined edge and 12 mm from the center
of the corneal endothelium).
Increased
endothelial cell density in the paracentral and peripheral regions
of the
human cornea.
Paull AC, Whikehart DR. Expression
of the p53 family
of proteins in central and peripheral
human corneal
endothelial cells.
Engelmann K, Bohnke M, Friedl P. Isolation and long - term cultivation
of human corneal
endothelial cells.
Neutrophils recruit and activate
Human Endothelial Colony Forming
Cells at the site
of vessel injury via PSGL - 1 and L - Selectin.
Hyperosmolarity attenuates TNF - α — mediated proinflammatory activation
of human pulmonary microvascular
endothelial cells.
A
Human Long Non-Coding RNA ALT1 Controls the
Cell Cycle
of Vascular
Endothelial Cells Via ACE2 and Cyclin D1 Pathway.
The impaired ability
of human corneal
endothelial cells (HCECs) to divide, both in vivo and in culture, has been well documented in the scientific literature for a number
of years [1 - 6].
Extended life
of human corneal
endothelial cells transfected with the SV40 large T antigen.
Initial imaging analysis
of fluorescently labelled
human iPSC - derived hepatic endoderm
cells, umbilical cord - derived
endothelial cells (HUVECs), and mesenchymal stem
cells (MSCs) co-cultured in a solidified matrix gel to promote 3D growth found that the different
cells collectively and automatically «condensed» into a multicellular central unit.
Specific inhibition
of AQP1 water channels in
human pulmonary microvascular
endothelial cells by small interfering RNAs.
Organ - on - a-chip technology may in part overcome this limitation, as exemplified by the «breathing» lung - on - a-chip that recapitulates the alveolar - capillary interface by co-culturing
human alveolar epithelial
cells and capillary
endothelial cells on opposite sides
of a flexible, porous, ECM - coated membrane.
Review
of «Antitumor Effects
of CD40 Ligand - Expressing
Endothelial Progenitor
Cells Derived From
Human Induced Pluripotent Stem
Cells in a Metastatic Breast Cancer Model» from Stem
Cells TM by Stuart P. Atkinson.
Expansion and maintenance
of human embryonic stem
cell - derived
endothelial cells by TGFbeta inhibition is Id1 dependent.
The Role
of MGAT5 in
Human Umbilical Vein
Endothelial Cells Possible Relevance to the Pathological Mechanism
of Preeclampsia.
Researchers from the laboratories
of Hiroshi Y. Yoshikawa and Hideki Taniguchi had previously demonstrated the in vitro formation
of a 3D transplantable liver «organ bud» from
human induced pluripotent stem
cells (iPSCs) co-cultured with mesenchymal and
endothelial progenitors, and allows for the growth
of a small vascularized and functional organ [1 - 3].
To assess the expression
of Tie2 and other Ang / Tie signalling molecules on
human pericytes, a microarray - based expression profiling
of human brain pericytes (BP), placenta pericytes, pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and
human umbilical vein
endothelial cells (HUVEC) was performed.
Anastellin, a fragment
of the first type III repeat
of fibronectin, inhibits extracellular signal - regulated kinase and causes G1 arrest in
human microvessel
endothelial cells.
The most potent
of these, known as effector memory T
cells, are activated by a group
of proteins known as
human leukocyte antigens (HLAs) on the surface
of endothelial cells lining the donated organ's blood vessels.
A team at the Harvard John A. Paulson School for Engineering and Applied Sciences (SEAS) and the Wyss Institute for Biologically Inspired Engineering at Harvard University has invented a method for 3D bioprinting thick vascularized tissue constructs composed
of human stem
cells, extracellular matrix, and circulatory channels lined with
endothelial blood vessel
cells.
The team identified the connections between rs9349379 and EDN1 by deleting a region
of DNA at the SNP in
human pluripotent stem
cells and then converting these immature
cells into
endothelial cells and vascular smooth muscle
cells.
Production
of factor VIII by
human liver sinusoidal
endothelial cells transplanted in immunodeficient uPA mice.
A team led by Young - sup Yoon, MD, PhD developed a new method for generating
endothelial cells, which make up the lining
of blood vessels, from
human induced pluripotent stem
cells..
Susan Amara, USA - «Regulation
of transporter function and trafficking by amphetamines, Structure - function relationships in excitatory amino acid transporters (EAATs), Modulation
of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses
of human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology
of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy
of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis
of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms
of Neurotophin Receptor Signaling Mark Coles, UK - Cellular differentiation,
human embryonic stem
cells, stromal
cells, haematopoietic stem
cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect
of beta adrenergic stimulation; cardiovascular function
of cGMP protein kinase Yu Huang, Hong Kong -
Endothelial and Metabolic Dysfunction, and Novel Biomarkers in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors in the Regulation
of Vascular Tone, Adipose Tissue Regulation
of Vascular Function in Obesity, Diabetes and Hypertension, Pharmacological Characterization
of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions
of Biologically Active Components
of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology
of G protein - coupled receptors, in particular opioid receptors, regulation
of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design
of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors
of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution
of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation
of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function
of GABA - A receptors; mode
of action
of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation
of expression and function
of the ATP - binding cassette (ABC) transporters
Figure 4 shows the mean survival curves obtained with the MTT assay in
human umbilical vein
endothelial cells (HUVEC), under conditions
of normal and low proliferation rates (20 % and 2 %
of FBS).
This effect on
cell survival was not
endothelial cell - specific, since IC50 values for kahweol treatment
of several
human tumoral
cell lines were similar to those obtained for HUVEC (results not shown).
Three recent experimental studies focused on low consumption / exposure.949596 In one study, 29 smokers each consumed a single cigarette, immediately after which they had a significant decrease in blood vessel output power and significant increase in blood vessel ageing level and remaining blood volume 25 minutes later, as markers
of atherosclerosis.94 In another study,
human coronary artery
endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation
of oxidant stress sensing transcription factor NFR2 and up - regulation
of cytochrome p450, considered to have a role in the development
of heart disease.95 These effects were not seen when heart
cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain
cells.96
Direct conversion or reprogramming
of human postnatal
cells into
endothelial cells (ECs), bypassing stem or progenitor
cell status, is crucial for regenerative medicine,
cell therapy, and pathophysiological investigation but has remained largely unexplored.