For the expansion and differentiation
of human keratinocyte stem cells for permanent skin restoration in victims of extensive burns.
Functional and phenotypic characterization
of human keratinocytes expanded in microcarrier culture.
Not exact matches
A comparison
of epidermal equivalents generated from iPSC, hESC and primary
human keratinocytes (skin cells) from skin biopsies showed no significant difference in their structural or functional properties compared with the outermost layer
of normal
human skin.
«We culture typical skin cell
of the epidermis, such as
human keratinocytes, in our dishes to form an artificial epidermis with all
of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.
Wei Long Ng explained: «The two - step bioprinting strategy involves the fabrication
of hierarchical porous collagen - based structures (that closely resembles the skin's dermal region), and deposition
of epidermal cells such as
keratinocytes and melanocytes at pre-defined positions on top
of the biomimetic dermal skin constructs, to create 3D in - vitro pigmented
human skin constructs.
Papillomaviruses are a diverse group
of DNA - based viruses that infect the skin and mucous membranes
of humans and a variety
of animals (replicating exclusively in
keratinocytes).
In
human skin,
keratinocytes, the cells found in the outer layer
of our skin known as the epidermis, soak up our naturally occurring melanosomes.
Homogenous and functional population
of keratinocytes and melanocytes derived from pluripotent stem cells were generated by designing a multi-step sequential protocol respecting the chronobiology
of epidermis formation during
human ontogenesis.
Residual transgenes present after piggyBac - mediated
human iPSC production affect the functionality
of keratinocytes derived from such iPSCs compared to iPSCs completely free
of transgene sequences
Ng explains, «The two - step bioprinting strategy involves the fabrication
of hierarchical porous collagen - based structures (that closely resembles the skin's dermal region), and deposition
of epidermal cells such as
keratinocytes and melanocytes at pre-defined positions on top
of the biomimetic dermal skin constructs, to create 3D in - vitro pigmented
human skin constructs.
Induction
of GITRL expression in
human keratinocytes by Th2 cytokines and TNF - α: implications for atopic dermatitis.
In fact, they repeatedly generated iPS cells from the tiny number
of keratinocytes attached to a single hair plucked from a
human scalp.
For the first set
of experiments, first author Trond Aasen, Ph.D., a postdoctoral researcher at the Center
of Regenerative Medicine in Barcelona, used viral vectors to slip the genes for the master regulators Oct4, Sox2, as well as Klf4 and c - Myc into
keratinocytes cultured from
human skin explants.
In a study published in Molecular & Cellular Proteomics, researchers at the University
of Freiburg led by Jorn Dengjel performed a global transcriptome and proteome profiling comparing primary DEB
keratinocytes to normal
human keratinocytes.
The research, described in the journal Stem Cells under the title «Reprogramming Postnatal
Human Epidermal
Keratinocytes Toward Functional Neural Crest Fates,» was supported by grants from the National Institutes
of Health.
Parsa R, Yang A, McKeon F, Green H. Association
of p63 with proliferative potential in normal and neoplastic
human keratinocytes.