Sentences with phrase «of immunocytochemistry»

During the 1980s, Marder led studies using antibodies and the technique of immunocytochemistry to reveal the presence of many different neuromodulators in nerve fibres around the STG of crabs, including serotonin, proctolin and GABA.

Not exact matches

Additional confirmation of knockout and pluripotency was performed using immunocytochemistry (Figure 2, Panel C).

Immunocytochemistry also confirmed reduced expression of CD44 in siTRF2 silenced HCT116 cells as compared to Scrambled siRNA when treated with increasing concentration of Bleomycin (Fig 4d).

Mitochondrial localization of complex II visualized by immunocytochemistry using ab14715.

siTRF2 transfected cells were treated with increasing concentrations of Bleomycin sulphate (0µM, 40µM and 80µM) and immunocytochemistry was performed as per laboratory standardized protocols [22].

The uptake and degradation of fibrils was analysed by quantitative light - and electron - microscopic immunocytochemistry and immunoreactivity was detectable in 86 + / -3 % cells within 6 h of culture.

Immunocytochemistry / Immunofluorescence of mixed neuron - glial cultures labelling rabbit GFAP (red channel) and chicken vimentin CPCA - Vim (green channel).

For immunocytochemistry and neurite growth assays, a plating density of 5 × 104 cells / well of a 24 - well plate was used.

Immunocytochemistry / Immunofluorescence analysis of human cardiac stem cells labeling Ki67 with ab16667 at 1/250 dilution.

(B) Neurite length of GDF5 - treated (200 ng / ml daily for 72 h) SH - SY5Y cells at 72 h. (C — F) p - Smad1 / 5 levels as determined by (C, D) Western blots or (E, F) immunocytochemistry in SH - SY5Y cells 24 h after transfection with control miRNA or miR - 181a inhibitor.

The in vivo expression of RPE65 was not detectable by immunocytochemistry.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

The antibodies listed above in the immunocytochemistry section were also used on eye sections together with a cocktail of antibodies generated against human - specific markers (HSM) to identify human cells (the Oka blood group antigen, mouse TRA -1-85, 1 ∶ 10 (a kind gift from Peter Andrews, University of Sheffield, Sheffield, UK) together with mouse human nuclear antigen, 1 ∶ 1000, Millipore).

Intracellular expression of human immunoglobulin in HB1.F3.H2IgG NSCs was visualized using immunocytochemistry.

Transmission electron microscopy with colloidal gold immunocytochemistry showed structural damage in young and aged microvessel endothelium of ApoE4 animals extended to the cytoplasm of perivascular cells, perivascular nerve terminals and hippocampal neurons and glial cells.

The initial aim of the pathology investigation was to carefully examine the spinal cord using immunocytochemistry and electron microscopy, as well as classical techniques, to confirm the pathology previously reported and to look for new clues to the pathogenesis and aetiology using the more modern techniques.