In the Intestine Chip, the epithelium forms villi - like projections lined by polarized epithelial cells that undergo multi-lineage differentiation similar to
that of intestinal organoids, however, these cells expose their apical surfaces to an open lumen and interface with endothelium.
Not exact matches
«The special thing about our scientific work on the
intestinal organoid is that we can observe its inner workings,» explains Dr. Tamara Zietek
of the Department
of Nutrition Physiology.
«This work provides a proof
of principle for using stem cell - derived human
intestinal organoids in a therapeutic setting.»
«We have shown that the hydrogel matrix helps the human
intestinal organoids (HIOs) engraft into the
intestinal tissue, that they differentiate and accelerate the healing
of the wound,» said Andrés J. García, Regents» Professor in Georgia Tech's Woodruff School
of Mechanical Engineering.
Beyond the
intestinal applications, the researchers are also studying the use
of hydrogels to deliver
organoids to damaged kidneys and lungs.
«Because the primary Small Intestine Chip recapitulates the physical microenvironment that cells experience inside the human body, such as fluid flow and cyclic peristalsis - like stretching motions, it exhibits a genome - wide gene expression profile that comes closer to its in vivo counterpart than that
of the same
intestinal cells grown as 3D
organoids,» said first - author Magdalena Kasendra, Ph.D., a former Postdoctoral Fellow on Ingber's team and now Principal Scientist at Emulate, Inc. in Boston.
To read and download the publication, see Enhanced Utilization
of Induced Pluripotent Stem Cell — Derived Human
Intestinal Organoids Using Microengineered Chips
The other one, the finding by the Clevers group in Utrecht that adult Lgr5 + stem cells from the intestine, grown 3D in the presence
of basement membrane matrix and a defined set
of niche factors, can develop into ever - expanding, genome - stable 3D
organoids that resemble the structure and function
of the original
intestinal epithelium (13 - 16).
The team then selected cells from these
organoids and placed them inside the Intestine - Chips, which are about the size
of AA batteries and re-create the natural microenvironment
of the human intestine, including the
intestinal epithelium — the layer
of cells that forms the lining
of both the small and large intestines.
ANN ARBOR, Mich — By combining engineered polymeric materials known as hydrogels with complex
intestinal tissue known as
organoids — made from human pluripotent stem cells — researchers have taken an important step toward creating a new technology for controlling the growth
of these
organoids and using them for treating wounds in the gut that can be caused by disorders such as inflammatory bowel disease (IBD).
The
intestinal organoids that grew from these elements developed several
of the singular characteristics
of that tissue type, including small knobby protrusions (crypts) that in full - size intestines serve as receptacles for stem cells.