The advantage
of isobutanol, he said, is that unlike some other biofuels, it does not freeze at 15,000 feet and continues burning very well in the subzero temperatures as high as 35,000 feet.
Moreover, among all the engineered strains examined, the strain with the combined modification V461I / S286T showed the highest (2.4 times) improvement
of isobutanol - to - 3M1B molar ratio, which was due to a decrease of the activity towards 3M1B production.
They managed to suppress the yeast's evolutionary self - interest by genetically engineering it to produce large quantities
of isobutanol.
But while those enzymes are active, ones that influence the production
of isobutanol can't work.
Not exact matches
Efforts to make
isobutanol from bacteria alone have been underway since 2000 when the U.S. Department
of Agriculture and the U.S. Department
of Energy (DOE) began distributing grants to universities that could demonstrate successful production
of liquid biofuels.
In one case, turning on and off a blue light caused the special yeast to alternate between producing ethanol, a product
of normal fermentation, and
isobutanol, a chemical that normally would kill yeast at sufficiently high concentration.
The researchers predicted they could use a combination
of genetic engineering and light to fine tune
isobutanol production.
A team
of researchers at Princeton University has developed a way to cause yeast to produce more
isobutanol, a possible candidate for use as a biofuel.
In the present study, we used protein engineering
of Kivd to improve
isobutanol production in Synechocystis PCC 6803.