Sentences with phrase «of isotype control»
HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
http://www.abcam.com/ Not exact matches
Use negative controls (unstained and isotype control antibodies) to determine gating of populations.
(d) Histogram of membrane - bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry.
(C) Survival of gp33 - immunized mice with LCMV infection following 30 d rest period compared with control mice with ST2 blockade or isotype control Ab treatment.
They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or isotype control antibody.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
To assess the ability of F3 - IgG to inhibit the proliferation of HER2 - overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 - IgG, or an isotype - matched control antibody.