Sentences with phrase «of kilobase»

This analysis revealed that a SNP called rs9349379, located hundreds of kilobases away, was the strongest risk factor for cardiovascular disease at chromosome 6p24.

Current array CGH strategies have limits of resolution that make detection of small (less than a few tens of kilobases) gains or losses of genomic DNA difficult to identify.

In truth, human chromosomes are tightly organized and packed into the nucleus, a situation which causes physical interaction between regions that might be hundreds of kilobases apart.

The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it.

Lindblad - Toh and a large group of investigators [31] compared the haplotype structure of the genome of the Boxer and 10 other breeds and found regions of linkage disequilibrium extending over several megabases within a breed and tens of kilobases between breeds.

Linkage disequilibrium may extend for megabases within some breeds, compared with only tens of kilobases in humans, making genome - wide studies more economical in dogs [7].

Not exact matches

Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli.

Long - range PCR is a method that allows for the amplification of much larger fragments of DNA than is possible with traditional PCR — fragments larger than 40 kilobases have been reported in long PCR, versus fewer than 10 kilobases for traditional PCR.

The human UFD1L gene was deleted in all 182 patients studied with 22q11 deletion, and a smaller deletion of approximately 20 kilobases that removed exons 1 to 3 ofUFD1L was found in one individual with features typical of 22q11 deletion syndrome.

The authors of this study have developed a universal primer set across flowering plants that amplifies 3 - 15 kilobase fragments, which can then easily be sequenced using recently developed next - generation sequencing technologies.

RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen.

This set of proteins can process seven of the eight base - base mismatches in a strand - specific reaction that is directed by the state of methylation of a single d (GATC) sequence located 1 kilobase from the mispair.

To test whether the P210 is the product of the novel 8.5 - kilobase bcr / abl fusion transcript, antibodies were prepared against c - abl and bcr determinants.

By using these reagents and v - abl - specific antisera, it was demonstrated that the P210 in CML cells is indeed the protein product of the 8.5 - kilobase transcript.

Abbreviations: bp, base pair; CS, Cockayne syndrome; E [number], embryonic day [number]; kb, kilobase; NER, nucleotide excision repair; SEM, standard error of the mean; TFIIH, basal transcription / DNA repair factor IIH; TTD, trichothiodystrophy; UV - RRS, recovery of RNA synthesis after ultraviolet irradiation; UV - UDS, unscheduled DNA synthesis after ultraviolet irradiation; wt, wild - type; XP, xeroderma pigmentosum; XPCS, xeroderma pigmentosum combined with Cockayne syndrome; XPTTD, combination xeroderma pigmentosum and trichothiodystrophy

One of the noncoding de novo mutations was 254 kilobases downstream of FGFR2, in a noncoding region that looks (according to chromatin marks) like a neural crest enhancer.

Negative frequency dependent selection acting at the locus responsible of self - incompatibility in Arabidopsis represents one of the strongest cases of balancing selection, and yet we have shown that its indirect effects on neighboring regions are restricted to a few kilobases [2].

Here we report a public database of common variation in the human genome: more than one million single nucleotide polymorphisms (SNPs) for which accurate and complete genotypes have been obtained in 269 DNA samples from four populations, including ten 500 - kilobase regions in which essentially all information about common DNA variation has been extracted.

The human T cell receptor beta (TRB, previously known as TCRB) gene locus on chromosome 7 (7q34) includes 65 Vb (variable) gene segments, followed by two separate clusters of genes each containing a Db (diversity) gene, several Jb (joining) genes, and a Cb (constant) region spread over 685 kilobases.

Unfortunately, LOH regions tend to be kilobases or megabases in size, containing dozens or hundreds of genes, and identifying which ones are truly affected in terms of cancer remains challenging.

The density of variants (an informal proxy for purifying selection) differed as well: 6.7 and 6.5 variants per kilobase in DHS peaks and footprints, respectively, compared to 4.2 variants per kilobase in the exome.

To compare the expression values across genes and conditions, raw count data was transformed into fragments per kilobase of exon per million fragments (FPKM) with the formula:

First, they sheared DNA into fragments that were a few kilobases long, ligated adapters to the ends of each fragment, and did a round of amplification.

Characterization of bacteriophage P1 library containing inserts of Drosophila DNA of 75 - 100 kilobase pairs D.A. Smoller, D. Petrov and D.L. Hartl Chromosoma (1991) 100:487 - 94

Reads exceeding 150 kilobases have been achieved, as have in - field detection and analysis of clinical pathogens.

Atsushi Nakabachi, a postdoctoral research associate in UA's department of ecology and evolutionary biology and a visiting scientist at RIKEN in Wako, Japan, is the first author on the research report, «The 160 - kilobase genome of the bacterial endosymbiont Carsonella.»