Sentences with phrase «of labeled antibodies»

«We showed that the presence of the maternal gut microbiota during late pregnancy blocked the passage of labeled antibodies from the circulation into the brain parenchyma of the growing fetus,» says first author Dr. Viorica Braniste at the Department of Microbiology, Tumor and Cell Biology at Karolinska Institutet.

Not exact matches

Generic labeling of proteins is achieved through fluorescent - antibody binding.

A radio - labeled human antibody binds to the viral gp41 protein expressed on the surface of the HIV - infected human lymphocyte and the cell is killed by alpha radiation delivered by the antibody.

The researchers selected one of the antibodies, labeled 3B11, as a «lead» candidate for further research.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

Brilliant Violet dye conjugated secondary antibodies from Jackson ImmunoResearch allow the addition of more colors to multiple labeling assays.

BV421 and BV480 are available conjugated to a range of secondary antibodies recommended for multiple labeling due to their minimal cross-reactivity.

Using an antibody that binds and labels a specific viral protein, the researchers tracked the virus as it first infected nerves in the gut 2 or 3 days after the nasal spray and then successively appeared in the brain stem and midbrain and ultimately infected much of the rest of the brain within 10 days.

Labeled antibodies, which bind to specific cell types, allowed the researchers to determine which types of neuron were being created at which times.

(E) R5 antibody labels Müller glia (Mü; red), which extend processes the breadth of the flank retina.

Difficult - To - Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental conditions.

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

Immunofluorescent analysis of HepG2 cells using 60008 -1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

ImmunoStar's 5 - HT6R Antibody demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using immunohistochemistry and immunofluorescence.

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004 -1-Ig (GAPDH antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

At 13 months of age, the intracellular MOAB - 2 immunolabel was hardly detectable in pyramidal neurons adjacent to amyloid plaques, the latter which were strongly labeled by this antibody and not by pab27576 (Additional file3: Figure S2 a-b).

PromoKine offers a wide range of well proven products for cell biology research such as kits and reagents for cell analysis, apoptosis research, cell transfection, fluorescent labeling, gene cloning & expression, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

Frozen sections of affected tissues were then stained with fluorescently - labeled Borrelia - specific polyclonal antibodies.

However, the morphology of the host inner retinal cells was well - preserved in the area of donor cell migration, as evident from the PKCα antibody staining, which labeled normal - appearing rod bipolar cell dendrites (upward arrows in Figure 6B).

The researchers used an antibody that labels the proteins made by two closely linked homeotic genes, Ubx and abdA, to show in which segments of the embryo the genes are turned on.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).

We make use of fluorescently - labeled antibodies and FACS machines to sort specific cellular populations.

To combat the nation's third deadliest cancer, the phase 1b / 2 open - label study will explore the combination of standard chemotherapy and two immunotherapy agents: an anti-PD-1 checkpoint inhibitor and a novel antibody targeting CD40, a protein that when activated can drive the immune system to attack tumors.

Determination of the expression pattern of antibody - or GFP - labelled puncta (presumptive UNC -7-containing gap junctions) in various mutant backgrounds was based on examination of at least two (typically more) independently isolated transgenic lines for each condition.

HuProt Cross-Section: microarray probed with a serum Ig sample and fluorescently - labeled secondary antibodies to the Ig and common epitopes of the arrayed proteins.

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Immunofluorescent analysis of Hela cells, using SMN2 antibody 11708 -1-AP at 1:25 dilution and Rhodamine - labeled goat anti-rabbit IgG (red).

Such an optical contrast exceeded the optical contrast of the fluorescent labels (that were targeted to C4 - 2B and HS - 5 cells using the same prostate cancer - specific PSMA antibody, see Figure 7B, D) by 31 times.

By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).

Approximately 10 % of the total IL7RαhiCcr6 + cells were brightly labeled with Thy1 - PE antibody compared with about 0.3 % of naive T cells (Figure 3D).

To quantitate the proportion of cells in the LN lymphatic sinuses at a given moment in time, we optimized an in vivo procedure to label lymph - exposed cells based on the established method of antibody pulse - labeling of blood - exposed cells (Cinamon et al., 2008).

While some vaccines may be labeled for a younger age, vaccinations given before 8 weeks of age may not offer puppies and kittens full protection because the presence of maternal antibodies is known to interfere with the development of active immunity.

2 2 Current Canine Guidelines for the Diagnosis, Prevention, and Management of Heartworm (Dirofilaria immitis) Infection in Dogs (revised January, 2012) perform well without refrigeration using an antigen - specific antibody labeled with colloidal gold.

For example, Boehringer Ingelheim's Protech C3 label suggests finishing the primary vaccination program at 10 weeks: http://www.infopest.com.au/extra/asp/infopest/nra/labels.asp?prodcode=51487 MSD Animal Health's Nobivac DHP Continuum vaccine label recommends final puppy vaccination at 10 weeks: http://www.infopest.com.au/extra/asp/infopest/nra/labels.asp?prodcode=59043 Virbac's Canigen DHA2P vaccine label recommends the second primary dose at 12 weeks also stating: «In situations where high maternal antibody and potential challenge is possible an additional vaccination should be considered at 16 weeks of age.»

(C) At the transition between relatively well preserved and completely absent (*) retinal architecture, loss of photoreceptors (cones are labeled red with hCAR antibody) is associated with accumulation of autofluorescent material (green) in the subretinal space and within the RPE (arrows).