In Thy1 - PE labeling experiments, Ccr6 KO mice showed reduced frequencies
of labeled cells, a result that was most significant for the Vγ4 + population (Figure 4G).
Graphs show mean fluorescence intensity (MFI)
of labeled cells.
Mouse heart with two sets
of labeled cell clones (red and green).
Not exact matches
Germline
cells are
labelled «immortal»: they have reproduced indefinitely since the beginning
of life.
Also, I was pleasantly surprised when I woke up Saturday morning to find that some
of my tomato plants I planted last weekend had sprouted!!!!! I was super nervous they weren't going to work out because I made a huge mess planting them and forgot to
label the
cells in the planter.
Producing «clean» meat by culturing
cells — instead
of raising or slaughtering animals - is a new frontier in food production that will require consumer education and transparent
labeling.
Meanwhile, researchers at Columbia University in New York are working on a way to add dozens
of color
labels to Raman - imaged
cells.
Groups
of red and green
labelled embryonic
cells in a zebrafish during their first migration in gastrulation.
To find it, the scientists injected the eight tumor - bearing mice with high levels
of labeled glutamine and glucose, another metabolic compound commonly linked to the growth
of pancreatic cancer
cells.
Using fluorescent
labels, they were able to track the fates
of marked and unmarked chromosomes under the microscope, from egg
cells and sperm to the dividing
cells of embryos after fertilization.
HeLa
cells, with proteins
labeled in blue and DNA in red, are responsible for over 20 percent
of the
cell line contaminations.
But it contains four pairs
of apparently duplicated images, even though they are
labeled as showing different
cell lines.
Cell processes are also determined by the chemical
labeling (or marks) and specific spatial organization
of our genomes, which are epigenetic characteristics — that is, nongenetic but nonetheless inheritable traits.
Greber and his team infected human
cells in culture with the chemically
labeled viruses, and observed the behavior
of the viral DNA during entry into
cells.
Now you know that if you take a mouse embryonic stem
cell that is, say,
labeled so all
of the developed
cells will be blue, and you inject it into a mouse blastocyst, you'll get a chimera.
Photo
of a living Brainbow zebrafish, taken by Zachary Tobias (a research technician in Weissman - Unni's lab), showing a brightly
labeled neuron with its
cell body (white) at bottom.
After initiating photoreceptor loss in the fish retinas, the researchers monitored the immune system's response by tracking the activity
of three types
of fluorescently
labeled immune
cells in and around the eye: neutrophils, microglia and peripheral macrophages.
We have designed a microfluidic device in which we can manipulate, lyse,
label, separate, and quantify the protein contents
of a single
cell using single - molecule fluorescence counting.
In this mouse model, mutations in Kras and p53 genes resulted in the formation
of individual tumor
cell populations that were
labeled with different colors.
A radio -
labeled human antibody binds to the viral gp41 protein expressed on the surface
of the HIV - infected human lymphocyte and the
cell is killed by alpha radiation delivered by the antibody.
By developing a new technique for
labeling the gene segments
of influenza viruses, researchers now know more about how influenza viruses enter the
cell and establish
cell co-infections — a major contributing factor to potential pandemic development.
The study, which was led by postdoc Gregor Pilz and PhD student Sara Bottes, used in vivo 2 - photon imaging and genetic
labeling of neural stem
cells in order to observe stem
cell divisions as they happened, and to follow the maturation
of new nerve
cells for up to two months.
It turned out that 80 %
of the BrdU -
labeled cells were also tagged with the enolase, confirming that the newborn
cells were in fact neurons.
The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they
label infected
cells with a fluorescent protein — to visualize the neural circuit downstream
of the ESP1 receptor, as well as providing an image
of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.
To test this, the team infected monocytes with a form
of Toxo,
labeled red, that can't reproduce, then introduced those
cells into mice.
«Now with a single injection, we can
label specific
cells with a variety
of colors within weeks after the injection.»
While researching the life cycle
of baker's yeast, Saccharomyces cerevisiae, Gottschling's team figured out a way to
label yeast so that they could spot genetic mistakes in daughter
cells.
Thanks to the
cell - impermeability
of the fluorogenic probe, it's possible to
label only proteins that appear on the
cell membrane.
It has long been known that
cells tag proteins for degradation by
labelling them with ubiquitin, a signal described as «the molecular kiss
of death.»
Accurately
labelling neurons with markers such as fluorescence, he says, will probably be the key challenge in the eventual goal
of creating a «census»
of different
cell types in the brain.
Biologists at the University
of Cambridge used fluorescent
labelling to stain
cell membranes in skate embryos, and tracked them through the dynamic development process.
These functions have made it possible to quickly fluorescence -
label only GLUT4 that is translocated to the
cell membrane by fluorogenic probes and clearly detect fluorescence
of GLUT4 translocation to the
cell membrane.
The technique, named
cell type specific
labeling using amino acid precursors (CTAP), exploits the inability
of vertebrate
cells to synthesize essential amino acids normally required for growth and homeostasis.
This method, known as targeted radionuclide therapy, or TRT, involves the use
of molecules
labeled with radioactive atoms that are injected into patients and localized in cancer
cells.
The researchers solved this problem by developing markers to
label the
cells of interest and then testing different enzymes and other reagents to draw them out
of tissues.
After they had injected the
labelled cells to mice, they tracked their locations in various phases
of the infarct.
The team went on to show that supplementing heavy stable isotope -
labeled forms
of these precursors led to incorporation
of heavy amino acids into proteins produced in enzyme expressing
cells.
Using quantitative mass spectrometry to search for proteins that contained these stable isotope
labels, researchers were able to determine the
cell of origin
of both intracellular and secreted proteins identified in multicellular culture.
They are examining every aspect
of cell function and
cell structure, looking for clusters
of phenotypes that could
label a patient's cancer so precisely that it could be linked to therapies proven effective against just that type.
The group
of imaging specialists led by Prof. Michael Schäfers, Coordinator
of the Cluster
of Excellence,
labelled the
cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method
of fluorescence reflectance imaging.
Using this, the researchers performed 3D super-resolution imaging
of stained structures in the
cells, and combined it with 3D
label - free phase imaging.
Recombinant proteins containing tetracysteine tags can be successively
labeled in living
cells with different colors
of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.
Rolls and her colleagues compared the ability
of fluorescently
labeled stem
cells from sleepy and from rested mice to migrate properly from the recipients» blood into the bone marrow.
«This technology allows for the
labeling of just one circulating pathological
cell among billions
of other normal blood
cells by ultrafast changing color
of photosensitive proteins inside the
cell in response to laser light,» explains Dr. Galanzha.
This image shows
cell death
of the human neural progenitor
cells (hNPCs) is mark by cleaved caspase 3 in red, the nuclei
of hNPCs are
labeled by DAPI in white / grey, and the ZIKA virus is
labeled by ZIKA virus envelope protein in green.
The chemical
labels newly divided
cells, and in their brain tissue, it showed up in a sprinkling
of neurons in the hippocampus — a seahorse - shaped structure involved in memory and learning.
Now, a new approach developed by Dr. Ekaterina Galanzha
of the University
of Arkansas for Medical Sciences in Little Rock and her colleagues allows for
labeling and tracking
of individual circulating cancer
cells throughout the body, thereby helping researchers elucidate the pathways
of single
cells from start to finish.
To better understand what happens during immune reactions in the body, researchers at Tübingen University have developed a new way
of labeling T -
cells, allowing them to track the T -
cell movement in mice using non-invasive positron emission technology (PET).
«We showed that the presence
of the maternal gut microbiota during late pregnancy blocked the passage
of labeled antibodies from the circulation into the brain parenchyma
of the growing fetus,» says first author Dr. Viorica Braniste at the Department
of Microbiology, Tumor and
Cell Biology at Karolinska Institutet.
They sent soil samples for DNA testing, looking for matches with particular genes known to be found in microbes and fungi; they tried to stimulate microbial growth on a wide variety
of substances and then count the
cells produced; and they used highly sensitive radiorespiration activity assays, which involve feeding the soil microorganisms a food source which has been
labelled with radioactive carbon, which can then be used to detect if the microorganisms are active.