Sentences with phrase «of luciferase expression»

In these assays, both wild - type Bach1 and FLAG - hBach1 - AP4 - 7 efficiently repressed basal levels of luciferase expression.

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Ten nanograms of reporter plasmid and 200 ng of miR7 expression plasmid were cotransfected with 1 ng of phRG - TK Renilla luciferase internal control plasmid (Promega) into 293TN cells using Lipofectamine 2000 reagent (Invitrogen).

p21waf1 / cip1 promoter — driven luciferase reporter (p21 - Luc; 0.05 - 0.5 μg) and increasing amount (0.1 - 2 μg) of FLAG - DDX3 expression construct were cotransfected into various cell lines as indicated.

As shown in Fig. 2A, exogenous expression of DDX3 in various cell lines led to a 2 - to 4-fold up - regulation of p21waf1 / cip1 promoter — driven luciferase activity.

To this end, increasing amounts of expression constructs of DDX3 / DQAD, DDX3 / AAA and wild - type DDX3 were cotransfected with p21waf1 / cip1 promoter — driven luciferase reporter.

All three compounds were able to induce luciferase expression, demonstrating their ability to overcome Bach1 repression of HMOX E2 - dependent transcription (Figure 6C).

Nonetheless, CDDO - Me and HPP - 1014 still were able to activate HMOX1 E2 - dependent luciferase expression in the presence of mutant Bach1 proteins, indicating that the CP motifs in Bach1 are not critical for efficient derepression of Bach1 by an electrophile (Figure 6D).

Expression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (FExpression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Fexpression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Figure 6A).

Luciferase expression was markedly lower in cells expressing Bach1, indicating effective repression of HMOX1 E2 - dependent transcription by Bach1 (Figure 6B).

In contrast, the ability of both CoPP and HPP - 4382 to induce HMOX1 E2 ARE - dependent luciferase expression in the presence of the mutant Bach1 protein was sharply inhibited.

When spleens from infected mice were examined for sustained control of infection at later time points, however, luciferase expression had rebounded.

Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteration.

Cells were transfected with 320 ng pGL3 luciferase expression construct containing the 3 ′ UTR of human APC, 40 ng pGL4.74 hRLuc / TK Renilla luciferase vector (Promega, Fitchburg, WI), and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).

The AG haplotype showed significantly greater expression of luciferase than the GT haplotype.

The minor allele of rs25532 significantly decreased luciferase reporter gene expression levels by 15 — 80 %, depending on 5 - HTTLPR allele background and cell type.