Sentences with phrase «of magnetic bead»

Using a proprietary protocol DNA fragments are bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.

For further information on MagSi - DNA cleanFIX and the fully range of magnetic beads available from AMSBIO please visit www.amsbio.com/DNA-sequencing-magnetic-purification.aspx or contact AMSBIO now on [phone] / [phone] or email [email protected].

We have a limited amount of product available for trial by users tired of the limitations of magnetic beads or who want to manipulate cells in a new way.

Using the new solution, DNA fragments are bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.

Not exact matches

With a variety of sounds, moving beads, a magnetic hand, crinkly ears, a peek - a-boo head, and an assortment of colours and textures, the fun and education will never end when you present your baby with this interactive Christmas gift.

The magnetic bead - based immunoassay panel facilitates detection of more samples than any other available assay, allowing researchers to get the greatest possible amount of data from each run.

So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts, that uses magnetic beads coated by an antibody that binds selectively and extremely tightly to the protein in the blood.

«We used magnetic beads of 3 micrometers in diameter to pull down the exosomes in plasma samples,» Zeng said.

First, IgE antibodies from the patient's blood are isolated by interaction with magnetic beads that are coated with a different type of antibody.

Each is composed of building blocks bound together, like a string of small magnetic beads — and just as magnets have poles, these building blocks have «plus» and «minus» ends.

Hiroshi Handa of the Tokyo Institute of Technology and his colleagues developed tiny magnetic beads — just 200 nanometers in diameter — that can be attached to drugs and other compounds.

A team led by the Hutch's Marie Bleakley and by Warren Shlomchik of the University of Pittsburgh School of Medicine in Pennsylvania removes naïve T cells from grafts with a magnetic system that uses monoclonal antibodies bound to iron beads.

If the sequence of interest is in the sample, it forms a link between the gold beads and the magnetic beads.

They added these beads to the sample to be tested, and then added that mixture to a test tube containing magnetic beads coated with DNA strands that bind the other half of the DNA.

Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

Evaluation of magnetic cellulose bead - based DNA extraction from faecal materials for high - throughput bacterial community analyses — Tanzila Afrin — Applied Entomology and Zoology

He still likes to tinker; only now he uses magnetic beads, atomic - force microscopes, and laser «tweezers» to explore the inner workings of the cell and the physical forces behind DNA replication.

This is a highly parallel sequencing approach that involves synthesizing short regions of identical DNA fragments on magnetic beads, packing millions of them into a chamber and then extending each of those molecules while detecting the addition of fluorescently labeled DNA building blocks or nucleotides.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.

With MagSi - Direct, you can start with a molecule of interest, attach magnetic beads to it and then use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.

With the aid of a magnetic plate holder, capture beads containing the hybridized mRNA were washed and incubated with provided labeling probes.

Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic beads separation.

High quality coating of magnetic silica beads with Steptavidin, Protein A, Protein G or other ligand specific molecules allows isolation of specific target molecules or cells out of large volumes or complex mixtures.

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

Following lysis and solid - phase capturing of mRNAs with streptavidin magnetic beads, samples were reverse transcribed using random primers.

HIF - 1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G magnetic beads (+).

This boho cuff is simply gorgeous with its multiple textured layers of seed beads, crystals, chains, and engraved magnetic clasp.

STRATEGIES COVERED Subitizing Counting on and counting back Use five / use ten Use doubles Get to ten Use ten and adjust Get to ten Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek Line

STRATEGIES COVERED Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance Double Use partial products Use five times Use ten times Double and half Factor and group flexibility Multiply up Partial quotients Use relationships INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of Picture & Dot Multiplication Cards 7 Flexible Grid Arrays 120 Bead Demonstration Magnetic Rekenrek Line