Get a set
of microplates (1.25 pound plates — or smaller).
Additional features
of the microplates include better well access when using water immersion, high numerical aperture objectives with an ultralow plate bottom, an improved lid design that reduces evaporation, corner spacers that avoid damaging the imaging surface when stacking, and a choice of different coatings to suit specific applications.
A rotational gripper enhances versatility in portrait or landscape orientation, while the unique lid - gripping method minimizes exposure
of the microplate's contents during transfer.
Future needs: Taking current and future trends into consideration is essential in maximizing the lifespan
of the microplate reader / detector.
In live, real - time cell - based experiments, it is beneficial to read from the bottom
of the microplate, rather than the top.
Currently, there are a wide variety
of microplate readers available, offering different capabilities and functionalities.
Not exact matches
But principally, knowing the timing
of the South China Sea's formation will let us better understand the interaction
of the many tectonic
microplates in South - East Asia.
They demonstrated a simple optical shutter based on arrays
of rotating
microplates, operating much like shutters on a window.
Using mapped surface geology, geophysical data, and thermochronology (i.e., time - temperature history
of the rocks), Fitzgerald and colleagues have determined that much
of Alaska's uplift and deformation began some 25 million years ago, when the Yakutat
microplate first started colliding with North America.
For each assay, 200 μl
of sample (taken from the Optisol solution bathing the human and rabbit corneal endothelial tissues) was added to
microplates coated with TGF - β receptor.
This study shows how
microplate respirometry can be successfully used to assess changes in respiration
of coral larvae, and our findings suggest that the effects
of thermal stress and nitrate enrichment in coral larvae may be species specific and are neither additive nor synergistic for O. faveolata or P. astreoides.
The DropArray
microplate only has walls around the outside
of the plate, which serve to contain wash buffers added in bulk during wash steps
of the assay.
We used a fluorescence - based
microplate respirometer to measure the oxygen consumption
of coral larvae from both species, and also assessed the effects
of these stressors on P. astreoides larval settlement and mortality.
Rather than using physical wells, DropArray
microplates are completely flat including a series
of 96 or 384 hydrophilic spots.
The Ultravap Mistral from Porvair Sciences is designed to remove the traditional laboratory «bottleneck»
of solvent evaporation from
microplates.
Then, the first objective
of the HTS platform is to develop a robust cell based assay in
microplates (96 - 384 - wells) with a specific read out.
With the high sensitivity and speed required for live cell and tissue imaging and the ease
of use and flexibility required for
microplate imaging and complex screening protocols, FV3000 brings complete workflows from live cell 2D - 4D imaging through image processing such as deconvolution, and analysis.
After 5 - 10 minutes the color development was stopped by addition
of sulphuric acid and the absorbance at 492 nm / 620 nm was measured in a
microplate reader.
Amsbio announces the launch
of two new
microplate - based metabolism stress kits enabling characterisation
of the main parameters
of mitochondrial function in live cells and evaluation
of the glycolytic response
of in vitro cell models to metabolic stress.
Visit the SelectScience
Microplate Readers / Detectors product directory for an overview
of the latest products from leading manufacturers.
Provided the possibility to address intracellular target engagement in
microplate format, thus allowing for large compound series to be addressed quantitatively, the researchers propose that such experimentation is included as a criterion for validation
of the action mechanism
of chemical probes.
From
microplate readers to imaging systems to software, our wide range
of solutions help scientists share their discoveries.
To determine confluence
of basic cell culture, many modern
microplate readers are equipped with a «confluence count» setting.
Microplate readers are used to detect biological, chemical or physical events in microtiter plates via the measurement
of light.
You will want to ensure that your chosen
microplate reader / detector is capable
of fulfilling all your requirements but also consider how easily upgradable it is for your future needs.
Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in
microplate readers, enabling in vitro and in vivo determination
of gene expression for studies using markers
of genetic alteration.
The CLARIOstar
microplate reader enabled calculation
of IC50 and KD values from saturation and competition binding assays.
Watch the video with Dr. Kirk McManus, Associate Professor, University
of Manitoba, and Senior Scientist, CancerCare Manitoba, to learn how temporal resolution
of 3D tumor spheroids in colorectal cancer is obtained using the Cytation
microplate reader by BioTek.
Microbiologist, Dr. Jason Sylvan, Assistant Professor
of Oceanography, Texas A&M University, who samples microbes from the depths
of ocean, measures microbial enzyme activity using the portable Spark ®
microplate reader by Tecan that he carries with him on the ship.
Software:
Microplate readers take hundreds or thousands
of measurements, so data acquisition and analysis are key to efficient workflow.
Whether purchasing a
microplate reader / detector for a new application or replacing an existing system, there are a number
of factors to consider.
In an exclusive interview with SelectScience, the founder
of Montana Molecular, Anne Marie Quinn, shares how her team measures, in real time, multiple GPCR signaling pathways inside a living cell using the CLARIOstar
microplate reader.
Spark brings together the capabilities
of an automated incubator, a
microplate reader and a cell imager in one, offering new possibilities for you and your lab.
These new options — combined with the ability to read 1 - to 1,536 - well
microplates — ensure there is a Spark configuration to increase the productivity
of your lab, both today and in the future.
SparkControl's kinetic conditioning feature allows you to program the system to automatically perform a required action as soon as a predefined signal is reached in a control well, fitting almost any assay workflow you can think
of, in un-lidded or lidded
microplates.
Tecan's Spark multimode
microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cel lconfluence, thereby boosting automation
of cell - based studies.
A typical multimode reader can only heat the
microplate chamber, usually requiring an offset
of 2 - 5 °C above the current room temperature.
BioTek offers a variety
of lightly used
microplate instrumentation, including multi-mode
microplate readers,
microplate washers and dispensers at significant.
Vent provinces are resolved comprising the Mid-Atlantic Ridge, the ESR, the northern, central, and southern East Pacific Rise, a further province located south
of the Easter
Microplate, four provinces in the western Pacific, and a further Indian Ocean province.