In their report receiving advance online release in Nature, the investigators describe evolved versions of the DNA - cutting Cas9 enzyme that are able to recognize a different range
of nucleic acid sequences than is possible with the naturally occurring form of Cas9 that has been used to date.
And a new prototype device can display a fluorescent letter in the presence
of nucleic acid sequences from the Ebola virus or the closely - related Marburg virus: «E» for Ebola or «M» for Marburg.
Here we present fCCAC, an application of functional canonical correlation analysis to assess covariance
of nucleic acid sequencing datasets such as chromatin immunoprecipitation followed by deep sequencing (ChIP - seq).
Not exact matches
Says Roberts: «He quickly figured out that if Illumina was successful, it would need more than the world's supply
of oligonucleotides» — the short snippets
of nucleic acid that are designed to pair up with specific genetic
sequences, and which are critical for DNA or RNA analysis.
This blockchain manager would «compute a cryptographic hash»
of a given «
nucleic acid sequence» and «use» it as PoW before transmitting the attendant «new block... to the other nodes in [the] blockchain system.»
According to one
of his analogies: just as the
sequence of letters on a page is extraneous to the chemistry
of ink and paper, so the
sequence of nucleic acids in the DNA molecule (which, when translated, determines the shape
of an organism and its specific characteristics) is extraneous to the chemical forces operative in the genetic process.
Still is it not possible that the specific
sequence of base - pairs in a DNA molecule is extraneous to the chemistry which bonds the
nucleic acids to one another?
So we must ask whether chemistry (or any physical science) can specify the overall
sequence of nucleic acids that determines the kinds and shapes
of organisms existing in the biosphere.
I have followed Polanyi's contention that there are organizational principles operative in the universe which formatively influence the specific
sequences of nucleic acids in DNA, and with Sheldrake I have postulated the existence
of morphogenetic fields which canalize the processes
of growth and development in organisms.
«It's difficult to make contrived samples behave like actual cell - free
nucleic acids, so that's been quite a challenge,» says Kelli Bramlett, director
of R&D in clinical
sequencing and oncology at Thermo Fisher Scientific in Waltham, Massachusetts.
«It's a type
of molecule called an antisense oligonucleotide, or ASO, that essentially is synthetic string
of nucleic acid that binds a specific
sequence in the gene.»
To start, she used a cotton swab to get a sample
of cells from her cheek, boiled them in a test tube in her kitchen to free the DNA, then added primers,
nucleic acids that mark the part
of the
sequence.
Using a combination
of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence
of a single
nucleic acid in a
sequence of DNA can lead to runaway weight gain.
Nucleic acid and protein
sequences, as well as three - dimensional structures
of proteins, are frequently the objects
of study, but gene expression as well as the simulation
of pathways and biochemical networks are attracting more and more attention.
A new
nucleic acid sequencing platform, the Sequel System, provides higher throughput, more scalability, a reduced footprint, and lower
sequencing project costs compared to the PacBio RS II System, while maintaining the existing benefits
of single molecule, real - time (SMRT) technology.
MicroRNAs are short bits
of nucleic acid — the material
of DNA and RNA — that can bind to messenger RNAs that code for protein because they have a complementary
sequence of A, U, C and G.
To try to block elastase, biochemist Drew Smith
of NeXstar Pharmaceuticals in Boulder, Colorado, earlier this year synthesized a
nucleic acid sequence called an aptamer, which is designed for its shape.
He won two Nobel Prizes for chemistry, but we claim him for molecular biology because the methods he developed for
sequencing proteins and
nucleic acids provide the basis for much
of what we do today.
Joint winner
of the 1980 chemistry prize with Walter Gilbert for his «contributions concerning the determination
of base
sequences in
nucleic acids» and winner
of the 1958 chemistry prize «for his work on the structure
of proteins, especially that
of insulin.»
Modern molecular technologies (genomics and other omics), through comparing
nucleic acid and amino
acid sequences across living species, are enabling the identification
of genetic components and patterns stingily conserved by evolution, from those in which times
of evolutionary branching
of the tree
of life can be inferred.
Over the past decade due to the deep -
sequencing technologies, we have studied the characterization
of nucleic acid alterations in a wide range
of cancers generating a large body
of information on how cancer develops and evolves.
In the 11 years before joining the CNAG he worked at the Centre National de Génotypage
of the Commissariat à l'Energie Atomique in Evry, France, first as Head
of Technology Development and later as Associate Director under Prof. Mark Lathrop.His research interests are high - throughput
nucleic acid analysis,
sequencing, SNP genotyping, genomics, genetics,
nucleic acid and protein analysis methods (molecular biological techniques and chemical modification), implementation
of methods, automation and analysis.
The predicted molecular weight
of serotonin transporter based on its
nucleic acid sequence is 67,815.
He'd like a way to perform single - cell analysis using microarrays, instead
of full
sequencing, but chip - based bisulfite methods require more
nucleic acid — about a microgram — than one cell can provide.
Sanger
sequencing methods represent the most popular in a range
of «first - generation»
nucleic acid sequencing technologies.
«It involves much more than a certain
nucleic acid sequence of DNA.»
Stepwise synthesis
of a
nucleic acid to determine nucleotide
sequences; inspired the DNA
sequencing technique by F.Sanger (Sanger, Nobel Prize Lecture 2004).
In 1999 the
nucleic acid sequence archives contained a total
of 3.5 billion nucleotides, slightly more than the length
of a single human genome; a decade later they contained more than 283 billion nucleotides, the length
of about 95 human genomes.
His current research focuses on chemical evolution
of sequence - defined carriers for drugs, proteins and therapeutic
nucleic acids, with a medical focus on tumor - targeted therapies.
The classic data
of bioinformatics include DNA
sequences of genes or full genomes; amino
acid sequences of proteins; and three - dimensional structures
of proteins,
nucleic acids and protein —
nucleic acid complexes.
We developed a way
of getting siRNAs into epithelial cells by either mixing them with a transfection lipid used to introduce exogenous
nucleic acids into cells in the lab or by adding a cholesterol tag to the end
of the RNA
sequence that allowed the RNA to be taken up into cells.
Our group seeks to investigate the use
of chemically - modified
nucleic acid aptamers, single stranded DNA or RNA
sequences that specifically bind to a diverse variety
of targets, in biosensing and catalysis.