Sentences with phrase «of open chromatin»

Every cancer starts with a single cell, and Jackson Laboratory (JAX) researchers have found a precise and reliable way — whole - genome profiling of open chromatin — to identify the kind of cell that leads to a given case of leukemia, a valuable key to cancer prognosis and outcome.

Regulatory elements tend to occur in regions of open chromatin (euchromatin), where they are accessible to transcription factors and other proteins.

The DNase I restriction enzyme cleaves regions of open chromatin, and it preferentially does so at places where, nearby, there are non-histone proteins bound to DNA.

It was previously believed that embryonic stem cells had lots of open chromatin (euchromatin), but this was not a proven theory.

This is consistent with a model in which DNA gain and loss results in turnover or «churning» in regulatory element dense regions of open chromatin, where interruption of regulatory elements is selected against.

The application of next - gen sequencing to RNA libraries (RNA - Seq), chromatin immunoprecipitates (CHiP - Seq), bisulfite - treated DNA, and regions of open chromatin (DNAse - Seq) makes it possible to interrogate many aspects of the genome in high - throughput fashion.

Having established some understanding of the open chromatin landscape in healthy mice, the researchers now hope to figure out how these relationships change with disease states.

Not exact matches

He said that the loss of Set2 keeps the chromatin in a more open state — not as compact as normal.

A large cast of biomolecular players finesses different swaths of this contorted chromatin into more closed or open shapes.

Depending on the tissue type and what jobs that tissue is doing, distinct portions of DNA are unspooled to become available for activity through a structure called open chromatin, said Gregory Crawford, an associate professor of pediatrics and expert on gene expression.

But what they found is that the host chooses which chromatin regions are opened to make genes available for use in each region of the intestine.

The researchers went into this study expecting to find that the microbes signaled the host to open up areas of chromatin to activate gene expression in the gut.

She likens TCF - 1 to an icebreaker ship that initially opens the ice (condensed, closed chromatin) and keeps a path available for other ships (other transcription factors that work in later stages of development) to steam through the now - open water (unwound chromatin).

«It has become very clear that the opening up or tightening of chromatin, to expose or hide genes in our chromosomes, plays a role in cancer progression,» dos Santos says.

Neocentromeres have also been shown to harbor expressed genes [13], and the rice finding shows that the chromatin structure of both plant and mammalian CenH3 - binding domains is open and accessible to the transcriptional machinery.

«Although histone 1 is key component of chromatin - the form in which DNA is packaged inside the cell nucleus through the action of histones -, there are still many questions open regarding this molecule,» says Ferran Azorín.

It turns out, as depicted by the model, that Ascl1 acts as a pioneer transcription factor that is capable of opening closed chromatin, and recruit the other factors to induce neuronal gene transcription.

What we think is that perhaps SOX2 is, even on a greater scale than Ascl1, capable of opening closed chromatin and thereby facilitating reprogramming.

Opening the way to accurately profile the chromatin states of in vivo stem cells, lineage progenitors and other scarce cell populations.

Chromatin's new identity as a non-transcriptional signaling agent opens up a plethora of research avenues.

TCF - 1 is like an icebreaker ship that initially opens the ice (condensed, closed chromatin) and keeps a path available for other ships (other transcription factors that work in later stages of development) to steam through the now - open water (unwound chromatin).

Key to these functions is its role as a sort of chromatin control panel — a chromatin «factor,» in research argot — regulating those openings, closings and shape - changings.

At sites of UV damage, E2F1 recruits the histone acetyltransferase GCN5, which acetylates histone H3K9 in order to open chromatin structure and increase accessibility to the damaged site by the DNA repair machinery.

However, the paper suggests this is due to epigenetic effects and chromatin remodelling, opening up a potential for epigenetic modulators in future treatment of RA.

ES cell chromatin is open at a global level [3], and the stem cells express high levels of chromatin remodeling factors.

Trowbridge hypothesized that analyzing open chromatin in bulk tumor cells could provide a possible improved method to identify cancer cell of origin because of the cell - type specificity of chromatin structure.