Sentences with phrase «of phosphatase»

Complete destruction of phosphatase is one method of testing to see if milk has been adequately pasteurized.

It may be because the extract boosts the activity of phosphatase enzymes, they think.

When she added small pieces of the phosphatase's extracellular domain to synoviocytes, the fragments occupied the binding sites on the proteoglycan molecules and thus prevented them from sequestering the phosphatase.

When Tonks and colleagues knocked down expression of the phosphatase PTPD2, the acini returned to their normal shape (right), even when HER2 signaling was activated.

When postdoctoral researcher and first author, Karen M. Doody, Ph.D., screened samples from rheumatoid arthritis patients for the expression of phosphatases, she discovered that an enzyme known as RPTPσ, short for receptor protein tyrosine phosphatase sigma, is highly expressed on the surface of FLS.

There have been a number of challenges with these new drugs, and they haven't really made it to phase three clinical trials, and so the reason is that it's very difficult to develop very specific inhibitors, because there is a large family of these phosphatases and it's difficult to ensure a specificity of your drug.

This phosphatase is a member of a large family of phosphatases, which are involved in dephosphorylating the insulin receptor.

In addition to causing the functional inactivation of these phosphatases, low concentrations of H2O2 or an oxidative shift in the GSH: GSSH redox status strongly increases the activity of the basic insulin receptor tyrosine kinase in the absence of insulin.

Not exact matches

Low dietary intake of copper may also be associated with increased fecal free radical production and fecal water alkaline phosphatase activity, risk factors for colon cancer.

Unfortunately, the high background of normal proteins, along with the presence of circulating phosphatases and other degradation enzymes, consistently stump researchers searching for these rare tumor biomarkers.

In this study, it is shown that dephosphorylation of Tyr15 triggered activation of the pp34 - cyclin complex from fission yeast, that a human protein - tyrosine phosphatase can catalyze this event both in vitro and in vivo, and that activation of fission yeast pp34 does not require threonine dephosphorylation.

The complementary DNA that encoded the tyrosine phosphatase replaced the mitotic activator p80cdc25, closely associating the cdc25 (+)- activating pathway with tyrosine dephosphorylation of pp34.

Page and his colleagues, who use animal models to understand how autism risk factors impact the developing brain and to identify potential treatments for the condition, have found that animals with mutations in the autism risk gene phosphatase and tensin homolog (Pten) mimic aspects of autism, including increased brain size, social deficits and increased repetitive behavior.

But, others say more work is needed to fully comprehend the role of prostatic acid phosphatase in viral transmission.

In this pathway, dopamine - and an adenosine 3 ′, 5 ′ - monophosphate (cAMP)-- regulated phospho - protein of 32 kilodaltons (DARPP - 32) is phosphorylated or dephosphorylated at three sites, in a pattern predicted to cause a synergistic inhibition of protein phosphatase — 1 and concomitant regulation of its downstream effector proteins glycogen synthesis kinase — 3 (GSK - 3), cAMP response element — binding protein (CREB), and c - Fos.

Ovaa and Neefjes» team decided to look at another class of proteins, called phosphatases, that act in the opposite way from kinases to see if inhibiting them would have a similar effect.

It is an enzyme — one in a «superfamily» of 105 called protein tyrosine phosphatases (PTPs)-- that perform the essential biochemical task of removing phosphate groups from amino acids called tyrosines in other proteins.

These enzymes include basic chitinase, which breaks down chitin — the major component of insects» hard, exterior exoskeletons — and purple acid phosphatase, which enables plants to obtain phosphorus, a critical nutrient, from victims» body parts.

The greater surface area of cluster roots increases root exudates of organic acids and phosphatases.

In animals harboring intraperitoneal hollow - fiber or subcutaneous implants containing light - inducible transgenic cells, the serum levels of the human glycoprotein secreted alkaline phosphatase could be remote - controlled with fiber optics or transdermally regulated through direct illumination.

The scientists then reversed this process and the synthetic liquid organelles reformed by adding another type of enzyme, called a phosphatase, which removed the phosphate group from the peptides, reestablishing their positive charge.

«All sorts of reactions in cells are controlled by modifying proteins with kinases and phosphatases,» said Keating.

«Two components of the pathway, the phosphatase PTPD2 and the lipid PA, are together required for HER2 signaling to function in mammary epithelial cells.»

Transmission of signals in a cell is controlled by the coordinated activity of two families of enzymes: protein tyrosine kinases, which add a phosphate group to proteins, and protein tyrosine phosphatases, which remove them.

Recent studies such as ours, however, establish protein phosphatases as positive and essential regulators of signal transduction, with remarkable substrate specificity and coordinated activities.

The phosphatases are also promising targets for therapeutic intervention in the treatment of various cancers,» Dr. Varjosalo states.

When administered to a preclinical model of arthritis, the phosphatase decoy ameliorated the symptoms of their disease.

«Researchers mapped interactions of key group of human proteins, the protein phosphatases.»

The protein phosphatases were too long thought to just be the negative counterpart of protein kinases, with promiscuous activity and low intrinsic substrate specificity.

The human protein phosphatases are illustrated in the form of the most well studied phosphatase, the protein phosphatase 2A (PP2A).

Furthermore, their study reveals novel physical as well as functional links to phosphatase - based regulation of human cancer.

Unlike with protein kinases, the current knowledge of protein phosphatase functions and especially on their formed interactions and complexes remains fragmentary.

A mutation in one of the genes that controls this pathway, PTEN (also known as phosphatase and tensin homolog), can cause a particular form of autism called macrocephaly / autism syndrome.

Pten (short for phosphatase and tensin homolog) is a tumor suppressor that is defective in about 20 - 25 percent of all patients with cancers.

In a paper published by Scientific Reports today, the research team found the activity of this protein, called PTEN (for Phosphatase and tensin homolog deleted on chromosome 10), is different between men and women.

The researchers also determined that SMEK1 doesn't act alone: it works in concert with Protein Phosphatase 4 to suppress the activity of a third protein called PAR3 that discourages neurogenesis, or the birth of new neurons.

Since alkaline phosphatase, coupled to the secondary antibody for the BrdU primary antibody, may have complicated the interpretation of the wounding experiments, we also treated human corneal - limbal tissues with BrdU histochemical assays in which fluorescein, rather than alkaline phosphatase, was coupled to the secondary antibody.

They show that it is a substrate for calcineurin, the phosphatase target of the immunosuppressive drugs cyclosporin A and FK506, and that it exists in activated cells as a complex with the transcription factor AP - 1 (Fos - Jun).

Similarly, Bernaudin et al. [43] found increased expression of 18 genes in the neonatal rat brain following hypoxia (8 % O2 for 3 h) including several known hypoxia inducible genes such as MAP kinase phosphatase - 1 (MKP - 1), several HIF - 1 target genes including VEGF and GLUT - 1, genes implicated in apoptosis, signal transduction molecules, and transcription factors.

PTEN phosphatase - independent maintenance of glandular morphology in a predictive colorectal cancer model system.

Tra2ß Protein Is Required for Tissue - specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon.

Carboxy - terminal domain phosphatase 1 silencing results in the inhibition of tumor formation ability in gastric cancer cells.

Flat mounts of the corneas were made, treated with BrdU, primary antibody, and then stained by reacting with alkaline phosphatase - linked secondary antibody.

Interestingly, alkaline phosphatase is also indicative of stem cell presence [29].

Ypi1, a positive regulator of nuclear protein phosphatase type 1 activity in Saccharomyces cerevisiae.

Thrombin - induced endothelial barrier disruption in intact microvessels: role of RhoA / Rho kinase - myosin phosphatase axis.

Novel function of the human presqualene diphosphate phosphatase as a type II phosphatidate phosphatase in phosphatidylcholine and triacylglyceride biosynthesis pathways.

Next they were covered on the endothelial side with a 1:10 dilution of anti-mouse-Ig secondary antibody coupled to alkaline phosphatase.

Research Focus: My research projects are focused on the roles of protein tyrosine phosphatases (PTPs) in diabetes and rheumatoid arthritis.

The DenA / DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation - Dependent Stability of Cytoplasmatic DenA / DEN1 during Fungal Development.