Sentences with phrase «of phospho»

When a victim dies because of Phospho - soda usage, his or her family members or heirs can file claims against the corporation for loss of life due to negligence.

(C) Measurement of Phospho - Erk levels in V+S + and V − S + fractions shows an enrichment of activated Erk with Venus positive ES cells.

Results: Simvastatin, but not pravastatin, reduced CSF levels... of phospho - tau - 181 (p - tau181) in all subjects.

A specific form of phospho protein phosphatase 2 regulates anaphase - promoting complex / cyclosome association with spindle poles.

Western blotting of phospho - Smad1 / 5/8 from zebrafish embryo extracts was previously described (35).

Not exact matches

In this pathway, dopamine - and an adenosine 3 ′, 5 ′ - monophosphate (cAMP)-- regulated phospho - protein of 32 kilodaltons (DARPP - 32) is phosphorylated or dephosphorylated at three sites, in a pattern predicted to cause a synergistic inhibition of protein phosphatase — 1 and concomitant regulation of its downstream effector proteins glycogen synthesis kinase — 3 (GSK - 3), cAMP response element — binding protein (CREB), and c - Fos.

Tim speculated that it might in fact work more like a «labeler,» tagging many diverse proteins with a «phospho - kiss of death.»

«How cells take out the trash: The «phospho - kiss of death» deciphered.»

We now know that HOPE makes this model also amenable to proteome and phospho - proteome analyses,» says Prof Michael Steinert, who coordinates a project on this topic supported by the German Federal Ministry of Education and Research.

Western blot analyses with phospho - specific antibodies suggested that stretching induces phosphorylation of ERK of the MAP kinase pathway, but did not induce phosphorylation of phosphatidylinositol 3 - kinase.

Glyceraldehyde -3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D - glyceraldehyde 3 - phosphate (G3P) into 3 - phospho - D - glyceroyl phosphate.

Phospho - Regulation of the Neurospora crassa Septation Initiation Network.

Figure 3A shows results of four control experiments, consisting of transgenic plants that lack one component of the complete signal transduction system (lack the receptor, lack the transmembrane HK, or lack the modified response regulator) or in which the critical phospho - accepting Asp53 residue was mutated.

In addition, the presenter will provide guidance on experimental design for multiplexed antibody panels combining phenotypic markers with phospho - specific intracellular targets, and discuss the use of fix and perm kits.

The routes of these multisite phosphorylation cascades are determined by precisely oriented docking interactions mediated by cyclin - specific docking motifs and by Cks1, the phospho - adaptor subunit of Cdk1.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

We further had to test a total of eight different antibodies against phospho - AKT and six different phospho - MAPK antibodies, before being able to detect BDNF signalling by immunofluorescence.

Importantly, the phospho - ERK1 / 2 signal was completely abrogated upon lethal toxin treatment, which also correlated with loss of MEK1 and MEK2 (Fig 4A).

We first assessed systemic levels of IIS signaling by performing western blot analyses to measure phospho - AKT levels in the wing discs.

For his Ph.D, he characterized the evolutionary dynamics of phosphorylation sites for functional phospho - proteomics and to understand the evolution of phosphorylation - based cell signaling.

and affinity of antibody ACI - 5400 were characterized by a panel of methods: (i) measuring the selectivity for a specific phospho - Tau epitope known to be associated with tauopathy, (ii) performing a combination of peptide and protein binding assays, (iii) staining of brain sections from mouse preclinical tauopathy models and from human subjects representing six different tauopathies, and (iv) evaluating the selective binding to pathological epitopes on extracts from tauopathy brains in non-denaturing sandwich assays.

Phosphorylation of signaling proteins was investigated using the following phospho - specific antibodies from Cell Signaling Technology: anti-pY1034 / 1035 JAK1 (# 3331S), anti-pY1007 / 1008 JAK2 (# 3771S), anti-pY705 STAT3 (# 9131S), anti-pY694 STAT5 (# 9351S) and anti-p44 / 42 MAPK (# 9101L).

In the second webinar, you will learn how to apply basic concepts of flow cytometry to topics such as cell proliferation, dead cell exclusion, and intracellular staining techniques for transcription factors and phospho - proteins.

Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right panel).

(A) Immunoblot illustration of the use of RIPK2 phospho - antibodies in two Hodgkin's lymphoma cells that have constitutive active RIPK2.

In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].

Phospho - proteomics of iPSC - based models to identify aberrantly regulated signaling pathways causing neurodegeneration.

Prior to working at City of Hope, she worked as a research associate in Dr. Peter Lee's lab at Stanford University since 2006 and facilitated in investigating mechanisms causing immune dysfunction in melanoma and breast cancer patients» peripheral blood, lymph node and tumor specimens utilizing techniques including microarray analysis, RT - PCR, Phospho - flow cytometry, histoloogy, and functional FACS studies.

Here we show ICV administration of VPA localized to the periventricular zone increased pro-survival phospho - proteins (pAkt (Ser473), pAkt (Thr308), pGSK3β (Ser9), pErk1 / 2 (Thr202 / Tyr204)-RRB- and growth cone associated proteins (2G13p, GAP43) in a whole animal system.

High levels of gene expression were found in both strains for genes involved in growth, energy and respiration (e.g., ribosomal proteins, ATP synthase, pseudoazurin), and the C - 1 metabolic carbon such as methanol dehydrogenase, ribulose monophosphate enzymes (D - arabino -3-hexulose 6 - phosphate formaldehyde - lyase, 6 - phospho -3-hexuloisomerase), formaldehyde activating enzymes (Data S6, Fig.

Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho - PDGF Receptor (Tyr754)(23B2) Rabbit.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

We know that many diseases are limited by heat — including influenza and a significant number of digestive diseases (heat breaks down virus phospho - lipid shells).

In late 2008, the product was recalled shortly after the FDA issued a health alert, warning that Fleet Phospho - soda posed a risk of causing kidney damage, and should only be available by prescription.

The FDA has never approved a double dosage of Fleet Phospho - soda for use in colonoscopy prep.