Sentences with phrase «of retroviral»

Having been excited by the potential of retroviral gene transfer as a therapeutic, it has been a fascinating opportunity to get to grips with the various processes in the lab; particularly more recently when I have spent time working independently to optimise the process in various cell subsets.

«The ethical issue of retroviral transfer from pig to man (which is in theory facilitated from organs of the double knockout pigs) remains a major one.

Long terminal repeats; U3 - R - U5 regions found on either side of a retroviral provirus (see below).

An experimental system for the evaluation of retroviral vector design to diminish the risk for proto - oncogene activation.

Evaluating the Safety of Retroviral Vectors Based on Insertional Oncogene Activation and Blocked Differentiation in Cultured Thymocytes.

Genetic Alterations: Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

Molecular and cell biology of retrovirus - host cell interactions — Development and application of retroviral gene transfer and drug screening systems

Harold Varmus, M.D., shared a Nobel Prize in Physiology or Medicine in 1989 with J. Michael Bishop, M.D. for their discovery of the cellular origin of retroviral oncogenes.

Reported to facilitate the annealing of retroviral RNA in vitro

While retroviruses generally only infect dividing cells (because their access to the host genome is thought to rely on the breakdown of the nuclear envelope that occurs in mitosis [Roe et al., 1993]-RRB-, lentiviruses are a genus of the retroviral family that can infect non-dividing cells (possibly through the use of nuclear localization signals by the viral components [Bukrinsky et al., 1992]-RRB-, and thus, offer an advantage when transducing certain cell types that exhibit limited cell division (e.g., neurons).

Sure enough, Perron saw faint traces of retroviral activity.

Because of the retroviral packaging signal, the ribozyme would be expected to colocalize with the lacZ - containing viral genomic RNA but not with the lacZ messenger RNA.

Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of recombination of selected markers; (ii) the transfer of the initially synthesized minus strand strong stop DNA was either intramolecular or intermolecular; (iii) the transfer of the first synthesized plus strand strong stop DNA was always intramolecular; and (iv) there was a strong correlation between the type of transfer of the minus strand strong stop DNA and the number of template switches observed.

This group is well known for its design of retroviral vectors for efficient gene transfer and it seemed like a good idea for me to go there for a year and exchange knowledge and expertise.

The aberrant and ongoing activation of ERVs caused an accumulation of retroviral proteins in the affected cells and deregulation of genes and pathways.

He previously spent 20 years in the U.S. Army's Medical Corps, heading its department of retroviral research.

«The integration of retroviral DNA is a relatively uncommon event compared with the number of viral DNA copies found in infected cells.

Not exact matches

These retroviral gene sequences make up about 8 per cent of the human genome, and are part of what is called non-coding DNA because they don't contain genetic instructions to make proteins.

With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.

Conditional knockout of Uhrf1, in a region of the forebrain, led to the activation of endogenous retroviral elements (ERV) in the genome, which are otherwise silenced by methylation.

The study, «VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer» found that novel non-coding parts of the human genome known as vlincRNAs (very long intergenic, non-coding RNAs) triggered by ancient viruses, participate in the biology of stem cells, and in the development of cancer.

At the end of the project, the transduction efficiency of the newly made retroviral vectors was tested in hematopoietic cells, and here my «old» techniques came in handy.

According to retroviral immunologist Judy Mikovits of the Whittemore Peterson Institute (WPI) in Reno, Nevada, xenotropic murine leukemia virus — related virus, or XMRV, was present in 68 out of 101 people with chronic fatigue syndrome, compared with only 8 of 218 healthy controls.

«Using genetic markers, people have been able to go far back and show that there were retroviral infections that were responsible for the creation of the placenta in mammals,» says Moore.

These data suggest that retroviral recombination is ordered and occurs during the synthesis of both minus and plus strand DNA.

The symposium features presentations by Philippa Marrack and John Kappler talking on the T cell repertoire; William Paul on interleukin 4 as a prototypic immunoregulatory cytokine; Timothy Springer on lymphocyte trafficking; Pamela Bjorkman on structural studies of MHC and MHC - related proteins, and Jack Strominger on peptide presentation by class I and II MHC proteins; Thierry Boon on genes coding for tumor rejection antigens, including the first tumor antigen, MAGE - 1; and Philip Greenberg on the modification of T cells for adoptive therapy by retroviral - mediated gene insertion Since then, the symposia series has attracted leading immunologists in the cancer vaccine and antibody fields, providing them with a comprehensive view of the promises and challenges in the development of cancer immunotherapies.

These cells were immortalized by retroviral transduction of the SV40 T antigen and Telomerase reverse transcriptase (TERT), the catalytic subunit of the telomerase complex [4], and then termed HMLE (Human Mammary with Large T and TERT).

\ nNow there are problems with these cells, perhaps due to retroviral vectors, absence of longevity or tumor suppressor genes, but this is no reason to give up.

«Conventional techniques of producing transgenic animals, such as microinjection of genes into eggs and the retroviral transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells.

Holic, N., Seye, A.K., Majdoul, S., Martin, S., Merten, O.W., Galy, A., Fenard, D.» Influence of mildly acidic pH conditions on the production of lentiviral and retroviral vectors.»

He also helped plot the retroviral genome and won election to the National Academy of Sciences.

1833 cells were incubated 48 hours with retroviral or lentiviral medium in the presence of Polybrene (8 μg / ml).

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).

In this study, we develop a reproducible protocol for efficient reprogramming mouse neural progenitor cells (NPCs) on human foreskin fibroblast (HFF) cells via retroviral transfer of human transcriptional factors OCT4 / SOX2 / KLF4 / C - MYC.

We also performed qRT - PCR to determine whether the retroviral OCT3 / 4 and KLF4 transgenes were silenced, which has been shown to be a reliable measure for efficient reprogramming of somatic cells into pluripotency [29].

Antibody responses particularly to Gag and Env proteins are hallmarks of immune responses to retroviral infections including experimental XMRV infection of macaques [22].

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

This report suggests it may be possible to reprogram human NSCs into iPS cells by OCT3 / 4 and kenpaullone, further minimizing the undesired effects of utilizing retroviral vectors.

All JAK1 and JAK2 mutants were generated using QuickChange XL II site - directed mutagenesis kit (Stratagene) and subcloned into the pMX - GFP (for JAK1) or pMEGIX - GFP (for JAK2) biscistronic retroviral vector upstream of the IRES as previously described.17 — 19 The mutated JAK1 and JAK2 constructs were verified by sequencing the full - length JAK1 and JAK2 using DYEnamic ET Dye Terminator Kit (Amersham Biosciences).

(A) Expression of pluripotency markers from iPSCs (HiPSC - 1 control) generated following retroviral transduction of unsorted HUF1 cells.

Given the methodologies used in 2003, versus what's being used today, even without knowing more, it would seem unlikely that the patent could be «formidable,» especially if it involves any retroviral integration of transcription factors.

In the original identification of XMRV in prostate cancer stromal cells, Urisman et al. [3] confirmed by sequence analysis that XMRV is not a laboratory contaminant, as is often the case with claims of new retroviral associations with disease.

Moreover, the viral sequences from XMRV - positive patients lacked the level of genetic diversity expected for retroviral infections [1], [14], implying that XMRV may have arisen from sample contamination and not true viral infection.

One of those is that they are more likely to have retroviral infections like FIV or feline leukemia (FeLV) than cats living in homes or feral cats.

Either way, Dr. Polak said, «all cats should be retroviral tested at the time of seizure from hoarding cases, as the retroviral status of these cats will influence housing decisions, whether or not you co-house them or individually house them.»

Our hospital follows the American Associations of Feline Practitioners (AAFP) Retroviral Testing Guidelines.

Dr. Jim Richards was leading the team of experts preparing this update on retroviral infections in cats when he suffered a fatal accident.

Dr Dodds also stated: «The T - cell leukaemias of human and animals are ex amples of those associated with retroviral infections.

I found that heartworm infection in cats often occurred at a similar rate with the retroviral diseases feline leukemia (FeLV) and feline immunodeficiency virus (FIV), as well as with respiratory disease and the presence of bites or abscesses.

The retroviral status of all cats should be known.