In 2012, JBEI researchers, including Blake Simmons, a co-author on this new study, had discovered a suite
of saccharification enzymes that were tolerant to ionic liquids.
Not exact matches
Saccharification efficiency is also associated with the loss
of specific sugars, and subsequent formation
of sugar breakdown products.
We then identified GH1 enzymes active at 70 °C and 20 % (v / v)[C2C1Im][OAc] over the course
of a 24 - h
saccharification reaction.
In this method, pretreatment
of corn fiber in a dilute acid solution at moderate temperature is followed by enzymatic
saccharification of cellulose, yielding up to 100 %
of the sugar content
of the hemicellulose without generating fermentation inhibitors.
Technological challenges remain, such as the need for more efficient enzymes for
saccharification, new microbes that can ferment multiple sugars, better integration
of process steps, and improved methods for recovery
of dilute ethanol.