Sentences with phrase «of skin biopsies»

Viral inclusions can also be demonstrated by immunoperoxidase staining of skin biopsies.

Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions

With the aid of thousands of skin biopsies and over a hundred kilograms of skin, researchers at Karolinska Institutet have observed how two subgroups of immune cell behave in healthy skin.

The new study was designed to see if a faster and better diagnosis could be made for transthyretin amyloidosis using a modified version of the skin biopsy technique.

Patients were excluded from this analysis if more than a single RFLP type was recovered on the culture of the skin biopsy specimen or if no blood culture was done.

To reduce confounding, the study analyzed persons from whom only a single RFLP type was recovered from a culture of a skin biopsy specimen.

Not exact matches

Oh yeah, and I was afraid of skin cancer so I had a dermatologist do about 4 biopsies.....

By analysing skin biopsies post-injection, the researchers found that the excessive inflammation was associated with activation of the p38 MAP kinase pathway.

«Furthermore, adapting in vivo corneal confocal microscopy for use in tracking HIV - induced PNS damage in patients may be of great value to identify early PNS damage independent of performing skin biopsies.»

Currently, skin biopsy is the accepted standard for measuring the loss of small, unmyelinated C fibers in the epidermis, one of the earliest detectable signs of peripheral nerve damage.

«The disability level aligned with the quantity of amyloid (protein clumps) present makes intuitive sense, but we were really amazed that the information from a small skin biopsy would correlate so well with disease severity,» says Polydefkis.

Johns Hopkins physicians report success in a small study of a modified skin biopsy that hastens the earlier diagnosis of an inherited and progressively fatal nerve disease and seems to offer a clearer view of the disorder's severity and progression.

The biopsies were thinly sliced and stained with a dye called Congo red that allowed the researchers to view the protein clumps in the layers of skin or within the sweat glands and pilomotor muscles (those that cause goose bumps) under the microscope.

In a report on their study, published June 20 as an Early View article online in Annals of Neurology, the Johns Hopkins team found that increasing levels of the protein clumps corresponded with worsening nerve damage, indicating that the smaller skin biopsies they used appear to work well as a measure of disease severity.

A skin biopsy on the girl's larger leg found 5 % of cells in that sample had the same abnormality, even though her blood sample was clean.

They also could see red protein clumps in the skin samples of these people, sparing them a more invasive nerve biopsy.

Finally, more than five years after her diagnosis of Wegener's, Luciana's lavender bumps led to another biopsy, this time of her skin.

Isolating M. leprae DNA was a challenging task, as the amount of bacteria in skin biopsies is generally low and varies greatly between patients.

A comparison of epidermal equivalents generated from iPSC, hESC and primary human keratinocytes (skin cells) from skin biopsies showed no significant difference in their structural or functional properties compared with the outermost layer of normal human skin.

Tissue engineers have been unable to grow epidermis with the functional barrier needed for drug testing, and have been further limited in producing an in vitro (lab) model for large - scale drug screening by the number of cells that can be grown from a single skin biopsy sample.

What we should be doing is taking a biopsy of the skin of melanoma patients and asking questions there,» says Paulos.

Doctoral student Stanley Cheuk and colleagues used up to 1,500 skin biopsies per experiment and a total of several hundred kilograms of healthy skin for the critical parts of the study.

In the new study, Janke and his research group examined the DNA evidence taken from skin biopsies of 190 giraffes collected by Fennessy and team all across Africa, including regions of civil unrest.

To identify this T cell population, the researchers took biopsies at the sites of active lesions before treatment and biopsies of the same skin areas after the lesions had cleared on therapy.

Potential harms of skin cancer screening include misdiagnosis, overdiagnosis, and the resulting cosmetic and — more rarely — functional adverse effects resulting from biopsy and overtreatment.

Seeking to establish links between populations of blue whales in the Gulf of Corcovado and other regions, the researchers examined DNA collected from the skin of blue whales with biopsy darts fired from crossbows across the eastern South Pacific.

A biopsy showed that the lump was malignant melanoma, a particularly fatal form of skin cancer.

Over the following week they took skin biopsies of the wound to identify where new skin cells had grown.

Ultra-deep genetic sequencing was performed on 234 biopsies taken from four patients revealing 3,760 mutations, with more than 100 cancer - associated mutations per square centimetre of skin.

To investigate the connection between immunity and fibrosis in scleroderma, the scientists looked at skin biopsies of scleroderma patients to identify factors responsible for persistent scarring.

Skin biopsies are then collected and reprogrammed into induced pluripotent stem (iPS) cells by the NYSCF Global Stem Cell Array, permitting Dr. Fossati and her team to generate patient specific neural cells, which will hopefully shed light on the mechanisms of disease progression.

Simao Cruz and colleagues report the clinical findings and the results of MRI, muscle biopsy, collagen VI expression in cultured skin fibroblasts and genetic tests of a series of patients with Bethlem myopathy.

They did this not through a cardiac biopsy, but by converting some of the boy's skin cells into induced pluripotent stem cells, and then into cardiac muscle cells.

The patient was treated on an acute lymphoblastic leukemia (ALL) protocol for eight cycles of hyperCVAD (cyclophosphamide, vincristine, adriamycin and decadron) alternating with MTX / Ara - C (methotrexate and cytarabine) and achieved complete remission (CR) confirmed by a bone marrow biopsy and by the resolution of most of her skin lesions.

They also took skin and blubber biopsies which helps with determining the gender of the whales along with what they have been eating.

Transcriptomic profiling of human skin biopsies in the clinical trial setting: A protocol for high quality RNA extraction from skin tumours

Skin biopsies (2 mm) were obtained from the advancing border of primary erythema migrans lesions, as described elsewhere [21].

Erythematous papules at the sites of tick feeding are shown in Panel A. Skin biopsies were taken distal to the bite site (Panel B) and within the papule (Panel C).

Given that there are millions of cells in a typical skin biopsy (researchers used skin from either the end of the tail or from the ear of the mouse), that translates into thousands of stem cells, each one capable of developing into any cell type of the body.

At one and two weeks after the completion of tick feeding, 4 mm skin biopsies were taken for PCR and culture.

So for instance, at Children's Hospital Boston we see a number of children who have heart problems, and it might be possible to take these cells directly from a skin biopsy and then in a dish turn them into heart cells.

Regrettably, the skin biopsies from this animal (IK14) were collected in the center of the EM and not the leading edge, which may have led to the absence of B. burgdorferi detection in these samples.

Half of the animals produced positive skin biopsy cultures and 8/10 were PCR positive.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

We hypothesize that SSEA3 expression is either identifying another less differentiated and / or tissue specific stem cell like population that is retrieved with the donor skin biopsy or that with culture, a subpopulation of fibroblasts may acquire the ability to express SSEA3.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

A nerve biopsy involves removing a small piece of peripheral nerve through an incision in the skin.

«The person may need a consult of (skin) biopsy to diagnose and rule out any other inflammatory skin disorders,» said Kim Nichols, M.D., a dermatologist and cosmetic surgeon in Greenwich, Conn..

Other breast cancer tests — diagnostic mammograms, ultrasounds, MRIs, and biopsies — are for women with symptoms who, for example, «feel a lump, have redness on the skin of their breast, or have discharge from the nipple,» says Dr. Jokich.

To perform the retrieval, a surgeon or urologist may conduct an epididymal aspiration (extraction of sperm with a needle through the skin), a testicular biopsy, or a complete removal of the testicles.