Sentences with phrase «of stem cell markers»

Phenotypic characterizations reveal high levels of CD44, α2β1 integrin, and β - catenin expression in holoclones, whereas meroclones and paraclones show markedly reduced expression of these stem cell markers.

Not exact matches

Previous research shows human induced pluripotent stem cells (hiPSCs)-- generated directly from adult cells — can express markers for a wide variety of cells, including those that secrete NP.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

They also showed that the cells have most of the surface «markers» considered as identifiers of embryonic stem cells and form «embryoid bodies» — characteristic clumps of cells formed by ESCs.

When Busch turned on the marker in adult animals, it became visible that at least one third (approximately 5000 cells) of a mouse's hematopoietic stem cells produce differentiated progenitor cells.

Three of the four projects receiving CIRM money capitalize on Victoria's expertise at finding markers for differentiated stem cells versus cells that still have tumor - forming potential.

Based on anatomy and molecular markers, we propose that it arose by duplication of a neighboring stem cell, which may have enabled finer neuronal control over leg - related behaviors, such as walking and grooming.»

Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.

They found that ONC201 alters the gene expression of cancer stem cell markers and signaling pathways prior to killing the tumor cells, providing pharmacodynamic biomarkers of response.

Dr Mohamed Elrayess, from ADLQ, said: «In this study we have shown that the impaired ability of fat stem cells to store excess fat was partially due to increased levels of the inflammatory marker interleukin - 6 in the blood.

A stem cell is generally characterized by the presence of various proteins — or cell markers — on its outer surface.

In a statement put on the Web this morning, NIH reported that all 64 lines «show characteristics of stem cell morphology» and have undergone several population doublings, and most of them have demonstrated all the protein markers «known to be associated with human embryonic stem cells.»

Whitehead Institute researchers have determined that the transcription factor Nanog, which plays a critical role in the self - renewal of embryonic stem cells, is expressed in a manner similar to other pluripotency markers.

Comparing the markers on these stem cells with those on the surfaces of cancer cells, Johnson adds, will also help scientists determine if stem cells contribute to prostate malignancies.

«We've not had cell surface markers for the different stem cell states before, which has made it hard to study them,» says Fredrik Lanner, assistant professor at Karolinska Institutet's Department of Clinical Science, Intervention and Technology.

To trace the lineage of liver cells, scientists used a telltale marker — the cells» response to signals delivered by a known stem - cell regulator called Wnt.

But on the right, the deletion of a MicroRNA called miR - 34a causes stem cells (green markers in the top right image and red markers in the bottom right image) to divide out of control.

A series of genetic markers sprinkled throughout the cells» chromosomes show the same pattern found in parthenogenetic mice as opposed to cloned mice, according to a report published online today in the journal Cell Stem Cell.

Mouse embryonic stem cells injected into rat brains express the AHD2 protein marker (yellow) characteristic of cells lost in Parkinson's disease.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Intrigued, the team inserted a human version of L1 into rat neural stem cells, along with a marker that would make the cells glow green whenever L1 made a jump.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

A section of a head and neck tumor — red and green markers show the proliferation of cancer stem cells — that formed when one of several newly characterized genes, Myh9, was suppressed.

Nearly 30 years after the discovery of the hematopoietic stem cell, Stanford researchers have found a marker that allows them to study the version of these stem cells that continues to replicate.

For example, labeling of these cells with stem - like cell associated markers (p63, nestin, and telomerase) have heavily implicated these cells as stem cells [13].

Middle: Immunofluorescence analysis of TRP1 (melanosomes marker) in melanocytes derived from pluripotent stem cells.

However, we have found a fluorescent marker for nestin, a neural crest stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).

Expression of the Wnt inhibitor Dickkopf - 1 is required for the induction of neural markers in mouse embryonic stem cells differentiating in response to retinoic acid.

When transplanted to the subretinal space of mice lacking functional photoreceptors, human embryonic stem cells directed toward a retinal lineage integrate into the outer nuclear layer, express photoreceptor markers, and restore a light response as determined by the electroretinogram (ERG)[5].

Methods: Human corneas with attached scleral rims were obtained from eye banks and were assayed for telomerase activity and BrdU (bromodeoxyridine) incorporation to determine, respectively, the presence of a stem - like cell marker and replicative activity.

Ihor Zahanich (Ravens, TUD)-- «Differentiation potential of human mesenchymal stem cells: expression of molecular markers, ion channels and gap junction channels» (2006)

Further research uncovered a broad spectrum of cell surface stem cell markers (e.g., CD133, CD44, and CD24) that allow the identification of CSCs in human solid tumors, including brain, breast, prostate, pancreas, liver, ovary, skin, colon cancers, and melanoma (3 - 6)(Figure 1 based on 7).

A lot of effort in tumor stem cell research has always been directed to finding a so - called marker, a molecule whose presence on a cell reliably identifies it as a cancer stem cell.

This gave a mixed population of cells with 20 % expressing CD34, a hematopoietic stem cell marker, with some co-expression of endothelial cell markers (CD31, Flk1, and VE - cadherin).

Pluripotent marker expression and differentiation of human second trimester mesenchymal stem cells.

Solid tumours are reported to possess a sub set of cells called Side Population expressing various stem like cell markers, self - renewal capacity and ability to give rise to differentiated cells [23, 24].

Review of «In Vitro Cell Motility as a Potential Mesenchymal Stem Cell Marker for Multipotency» from Stem Cells TM by Stuart P. Atkinson

Significance of CD44 and CD24 as Cancer Stem Cell Markers: An Enduring Ambiguity.

Biological markers of Mesenchymal Stromal Cells as Predictors of response to Autologous Stem Cell Transplantation in Patients with Amyotrophic Lateral Sclerosis; an Investigator - Initiated Trial and in Vivo Study.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

Title: Single - molecule transcript counting of stem - cell markers in the mouse intestine Authors: Itzkovitz S, Lyubimova A, Blat IC, Maynard M, van Es J, Lees J, Jacks T, Clevers H, van Oudenaarden A Date: 2011 Publication Details: Nat Cell Bcell markers in the mouse intestine Authors: Itzkovitz S, Lyubimova A, Blat IC, Maynard M, van Es J, Lees J, Jacks T, Clevers H, van Oudenaarden A Date: 2011 Publication Details: Nat Cell BCell Biol.

Now, in a study published in Stem Cells, the laboratory of Seung Hyun Kim (Hanyang University, Seoul, Korea) report on an open ‐ label, single ‐ arm, investigator ‐ initiated trial to identify biological markers of MSCs to predict response to in patients with ALS [3].

However, as demonstrated by a recent report from Stuelten and colleagues [13], the complexity of CSC markers continues to pose challenges for identifying and isolating the putative tumor stem cell populations by the cell - sorting approach.

Review of «Biological markers of Mesenchymal Stromal Cells as Predictors of response to Autologous Stem Cell Transplantation in Patients with Amyotrophic Lateral Sclerosis; an Investigator - Initiated Trial and in Vivo Study» from Stem Cells by Stuart P. Atkinson

(C) Confocal image of P150 dystrophic inner retina transplanted with hNPCctx — GDNF and double stained with antibodies against nestin, a neural stem and progenitor cell marker (green) and human nuclear antigen (red).

Neural crest - like stem cells (NCLSC) share many properties with aggressive melanoma cells, such as high migratory capabilities and expression of neural crest markers.

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

The expression of the tumoral multidrug resistance gene (MDR1), which encodes P - glycoprotein, and NANOG, a stem - cell marker and a regulator of the expression of drug resistance genes, was determined using qRT - PCR.

Of note, mouse neural stem cells (NSCs) were reprogrammed using only one (Oct4) or two factors (Oct4 and Klf4), due to the endogenously high expression of pluripotent genes, such as Sox2 and c - Myc, as well as several intermediate reprogramming markers [14], [17], [18Of note, mouse neural stem cells (NSCs) were reprogrammed using only one (Oct4) or two factors (Oct4 and Klf4), due to the endogenously high expression of pluripotent genes, such as Sox2 and c - Myc, as well as several intermediate reprogramming markers [14], [17], [18of pluripotent genes, such as Sox2 and c - Myc, as well as several intermediate reprogramming markers [14], [17], [18].

Support to date for the existence of an hypoxic niche in marrow blood stem cells came from indirect evidence such as expression of the hypoxia protein Hif - 1 and related genes and staining with surrogate markers of hypoxia.