«By offering the distinct advantage
of subcellular resolution and avoiding the undesirable influence of fluorescent dyes, we believe our technique can complement FDG in clinical PET imaging for visualizing glucose uptake activity at the cellular level,» says the lead author Fanghao Hu, a Ph.D. candidate in chemistry.
Not exact matches
«The cytoskeleton system is comprised
of a host
of interacting
subcellular structures and proteins, and our technique will enable research on the interactions between these different targets with unprecedented number
of color channels and spatial
resolution,» he said.
«We expect that our new method will become an attractive tool to study energy requirements
of living systems with
subcellular resolution,» says Min, «especially in brain and malignant tumors that are in high demand
of energy.»
A high -
resolution map
of the human cell has been generated — part
of the Human Protein Atlas database — that provides the in situ localization
of 12,036 human proteins at a single - cell level, covering 30
subcellular structures, and enabling 14 major organelle proteomes to be defined.
That technology rapidly and repeatedly sweeps an ultra-thin sheet
of light through the cell while acquiring a series
of 2 - D images, building a high -
resolution 3 - D movie
of subcellular dynamics.
Korenberg's team is developing a 3D coordinate system to align various types
of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas,
subcellular resolution with electron microscopy.
This technique allows us to capture images
of biological processes, for instance to trace individual tumor cells in mice for several weeks at
subcellular resolution.
Quantitative single cell monitoring
of protein synthesis at
subcellular resolution using fluorescently labeled tRNA.
Deputy Editor Steven Carr
of The Broad Institute will talk about new approaches for
subcellular localization
of proteins and their interaction partners with high spatial
resolution.
He works with a group on the Cell Atlas, a high
resolution subcellular atlas showing the spatial distribution
of proteins within cells.
In addition, we prepared a large number
of tools to manipulate signalling networks and are able to locally activate the important messenger such as PIP3 and DAG with a light flash in
subcellular resolution in living cells (Mentel et al. 2011; Nadler et al. 2013, Nadler et al. submitted).
The combination allows for the study
of 3D
subcellular processes in their native multicellular environments at high spatiotemporal (space and time)
resolution.