Sentences with phrase «of the cell line before»

Later, during a chance discussion with European colleagues, stem cell researcher George Daley of Children's Hospital Boston and the Harvard Stem Cell Institute learned that they had received samples of the cell line before the work was retracted.

Not exact matches

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

But the bottom line is that about two thirds of all T cells reside in the lymphoid tissue of the gut, where the virus spreads after exposure, even before it shows up in blood.

In these instances, the committee says, the use of existing embryonic cell lines derived from embryos fewer than 14 days old should be considered before resorting to the use of cells taken from surplus in vitro fertilization embryos fewer than 14 days old.

Today, MD Anderson urges all its scientists to fingerprint new cell lines as soon as they arrive in the lab and before publishing any data, and to complete an annual revalidation of all lines in their laboratories.

Until 2009, Saitou's starting point had been cells taken from a live mouse epiblast — a cup - like collection of cells lining one end of the embryo that forms at the end of the first week of development, just before the PGCs emerge.

Top series: Images show the elongated shape of cells transforming into a ball shape before they shed from the lining of the airway to cause obstruction, a key reason why RSV leads to bronchiolitis.

As acting NIH Director Raynard Kington explained at a press conference, there will be separate channels for determining whether a cell line is eligible depending on whether it was derived before or after 7 July, the effective date of the guidelines.

Pickles added that suppressing the effects of the RSV NS2 protein may also allow our immune system more time to deal with the RSV infection before the small airways become clogged with cells shedding from the lining of the airway.

The researchers then differentiated the iPS cells into endothelial cells, which line the interior of the heart and blood vessels, before transplantation.

The research, the team said, should not discourage the pursuit of experimental treatments but instead be heeded as a call to screen rigorously all cell lines for mutations at various stages of development as well as immediately before transplantation.

In some organisms, the assignment of a cell to the germ line is caused by an inheritance - based mechanism: Before there is even an embryo, the molecular content of some cells predetermines them to develop as either germ or soma.

The high quality of the new lines should make them appealing for clinical trials, says Braude, but it could take several years before the cells make their way into humans — if they ever do.

Before dividing, pairs of identical chromosomes line up in the middle of the elongated cell, and the microtubules, emanating out from the centrioles on either side, help pull the chromosomes in opposite directions so that each new cell receives one member of each chromosome pair.

Nonetheless, passage of the measure marks a significant milestone in efforts to overturn the Bush policy, which limits government funding to research using 60 human ES cell lines derived before 9 August, 2001.

Mycoplasma contamination has been shown to arise from a variety of sources such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy.

In terms of making the monoclonal line: I had never done it before and it worked the first time probably because I used a robust cell line.

«Before, you could in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue culture plates.

Acute myeloid leukemia (AML) is the leading cause of leukemia mortality in the United States.1 Curative treatment involves intensive induction chemotherapy, before proceeding to either consolidation chemotherapy or allogeneic stem cell transplantation based on the patient's risk for relapse.2 This approach has been employed for > 4 decades and, although most individuals achieve complete remissions with front - line therapy, 3 the majority of patients ultimately relapse with drug - resistant disease, and overall survival rates remain disappointingly poor.4 The limited ability of many patients to tolerate the intense chemotherapy - based treatments, in particular hematological toxicity, further contributes to the poor outcomes noted in this disease.

Before you have your period the endometrial cells that form the lining of your uterus produce lots of prostaglandins.

Honda says the price will be about $ 60,000, which is in line what what Toyota charges (before incentives of up to $ 13,000) to buy the Toyota Mirai fuel cell car.

The DeskGen software sifts through gigabytes of data, and allows users to modify cell line genomes in ways that have not been possible before.