Feric and Brangwynne study the mechanics
of the cell nucleus using eggs from the African clawed frog, Xenopus laevis.
Not exact matches
The OAR proposal
uses a variation
of therapeutic cloning called altered nuclear transfer (ANT) in which the
nucleus of a donor
cell (a skin
cell, for example), containing the 30,000 genes
of the genetic code, is altered in such a way that it produces an epigenetic factor, a protein called nanog.
As specialized
cells are generated during embryonic development, they are programmed to
use only a limited amount
of the total genetic information present in the
nucleus to produce the proteins required for their particular function.
To solve this, West proposed «therapeutic cloning» — taking the
nucleus out
of a patient's
cell, transferring it into an egg
cell to create a cloned embryo, then
using that embryo to derive patient - matched stem -
cell lines.
The prize will be equally split between biophysicist Venkatraman Ramakrishnan
of the MRC Laboratory
of Molecular Biology in Cambridge in England, biochemist Thomas Steitz
of Yale University and molecular biologist Ada Yonath
of the Weizmann Institute
of Science in Rehovot, Israel, for their work in
using x-ray crystallography to get a precise, atomic - scale map
of the ribosome — the protein - making machine in all
cells with
nuclei that makes life possible.
And while cytochalasin D might not be the actual agent scientists
use to trigger bone formation in the clinic, Rubin's study shows that triggering actin transport into the
nuclei of cells may be a good way to force mesenchymal stem
cells to become bone
cells.
Research shows that in Parkinson's disease a brainstem region called the pedunculopontine
nucleus (PPN) develops changes in DNA found in mitochondria — the batteries
of the
cell — as they produce and store energy that
cells can
use.
«
Using this elegant method, we can reveal that not all the incoming viral DNA enters the
cell nucleus as originally expected, but a significant fraction remains in the cytosol, the fluids
of the cytoplasm,» explains Greber.
Once a cluster
of supervoxels has been identified as a
cell nucleus, the computer
uses that information to find the
nucleus again in subsequent images.
Zhong Zhong and Hua Hua are the product
of somatic
cell nuclear transfer (SCNT), the technique
used to create Dolly the sheep over 20 years ago, in which researchers remove the
nucleus from an egg
cell and replace it with another
nucleus from differentiated body
cells.
Using this marker, the green fluorescence
of the
nucleus increases progressively in
cells in the growth phase and turns off after the division.
Luiz Da Silva and colleagues from the University
of California at Berkeley has already succeeded in
using the Livermore laser to observe 50 - nanometre features in the
nuclei of dried rat sperm
cells (Science, vol 258, p 269).
Salk scientists were able to selectively add fluorescent proteins to the
nuclei of motor neurons (red)
using cutting - edge genetic techniques to show how
cells in the spinal cord synchronize many neurons at once to allow complex movements.
The Ostrer study
used DNA from the
nucleus of the
cell in its analyses, and the Behar study
used both nuclear and mitochondrial DNA (mtDNA); the latter comes from tiny bodies in the living
cell that provide it with energy.
Dolly was cloned
using the technique
of «somatic
cell nuclear transfer,» when a
nucleus from an adult
cell is transferred into an unfertilized egg that has had its
nucleus removed, and is then shocked with electricity to start
cell growth.
In 1996 researchers in Scotland
used SCNT to transplant the
nucleus of an adult sheep
cell into an enucleated sheep egg, producing Dolly, the first cloned mammal.
In the early 1950s, Robert Briggs and Thomas King repeated Spemann's experiments
using a species
of leopard frog, Rana pipiens, first with a
nucleus from young embryos (Briggs and King, 1952) then from older embryos (King and Briggs, 1954); both the younger and older implanted
nuclei could still be reprogrammed by the enucleated host
cell.
DAPI was
used to stain the
cell nuclei (colored blue) at a concentration
of 1.43 µM for 1hour at room temperature
Each set
of 2 - D images was
used to calculate a 3 - D reconstruction
of a
cell detailing the changing chromatin formations in the
nuclei.
Over the past two decades, researchers have
used various AAVs as vehicles to transport specific genes into the
nuclei of cells; once there, the genes can be expressed, or translated, from DNA into proteins.
Researchers studying genome and
cell biology provide evidence that heterochromatin organizes large parts
of the genome into specific regions
of the
nucleus using liquid - liquid phase separation, a mechanism well known in physics but whose importance for biology has only recently been revealed.
Abstract:
Using a mulit - reflection time -
of - flight mass spectrograph (MRTOF - MS) located after a gas
cell coupled with the gas - filled recoil ion separator GARIS - II, the masses
of several heavy
nuclei have been directly and precisely measured.
For instance, a science teacher might get kids to
use their bodily - kinesthetic abilities by having them perform a lesson, representing a virus or the
nucleus of a
cell.
Ian Wilmut and Keith Campbell managed to clone the sheep Dolly,
using method
of somatic
cell nucleus transfer.