Not exact matches
Efficiency of transfection, as assessed by cotransfection with GFP constructs and subsequent GFP detection, was estimated at 60 % — 70 %.
In general, the higher the passage number
of a culture the lower the
transfection and transduction
efficiencies.
Improving Viability and
Transfection Efficiency with Human Umbilical Cord Wharton's Jelly Cells Through Use
of a ROCK Inhibitor.
Additionally, the Invitrogen ™ Neon ™
Transfection System offers non-viral delivery
of DNA, RNA, and protein efficiently into a variety
of blood cells, including human primary T cells, and achieves more than 90 % knockout
efficiency with CRISPR / Cas9 Ribonucleoprotein delivery.
To achieve sparse labeling
of individual neurons one could reduce the plasmids amounts to reduce
transfection efficiency.
Luciferase assays were analyzed based on the ratio
of Firefly / Renilla to normalize cell number and
transfection efficiency.
The co-delivery
of small molecular drugs with nucleic acids can improve gene
transfection efficiency, reduce side - effects
of these drugs, and achieve the synergistic effect
of drug and gene therapy for the more effective treatment
of cancer.
Unmatched performance — high
transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division
of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked