Sentences with phrase «old mice using»

Finally, we examined LTP at corticostriatal synapses in 6 - month old mice using a Hebbian protocol consisting of 4 trains of 100 Hz presynaptic stimulation paired with postsynaptic depolarization.

Investigators (Fumitaka Sato, PhD, et al) tested resveratrol in autoimmune and viral models of MS.. In the autoimmune model, experimental autoimmune encephalomyelitis (EAE) was induced in 6 - week - old mice using myelin oligodendrocyte glycoprotein (MOG) 35 - 55 peptide.

Researchers at Oxford have demonstrated that autophagy is diminished in T cells from aged mice, and T cell responses could be boosted in older mice using the autophagy - inducing compound spermidine.

Not exact matches

Using a surgical technique called parabiosis to join the circulatory system of a young mouse with that of an old one, the researchers studied the effects of young blood on elderly cells, and vice versa.

Wagers, Lee, and their colleagues used parabiosis to yoke together five 2 - year - old mice (downright ancient in mouse years) with 2 - month - old counterparts.

They used a somewhat bizarre technique in which two mice were sutured together in such as way that they shared a circulatory system (known as parabiosis), and found old mice joined to their youthful counterparts showed changes in gene activity in a brain region called the hippocampus as well as increased neural connections and enhanced «synaptic plasticity» — a mechanism believed to underlie learning and memory in which the strength of neural connections change in response to experience.

«It's remarkable, using a lowly house mouse to monitor a major milestone in human history,» says Melinda Zeder, curator of Old World archaeology at the Smithsonian National Museum of Natural History in Washington, D.C., who wasn't involved with the study.

Next, Finlay's group used stool samples from the asthma - prone 3 - month - olds to colonize the guts of mice that had been raised in a bacteria - free environment.

The team used only older, female mice because ADDE most commonly strikes that demographic in humans.

This visual abstract represents the findings of Thevaranjan et al. who, using young and old germ - free and conventional mice, demonstrate that age - related microbiota changes drive intestinal permeability, age - associated inflammation, and decreased macrophage function.

Changes in muscle repair with aging were determined by injecting the old mice and young mice (neither group exercised) with snake venom commonly used to induce muscle injury in rodent studies.

The researchers harvested satellite cells from both healthy and injured muscle tissue of young mice and from healthy tissue of old mice; extracted these cells» DNA with the histone coatings intact; and used tagged antibodies targeting the different kinds of marks to find which spots on those histones were flagged with either «stop» or «go» signals.

That conclusion was further supported by the results of an experiment in which the same old Tg pancreatic homogenate used previously was inoculated into WT, non-Tg mice of the same background.

In these experiments, all transfers were carried out using neonatal mice less than 24 hours old to mothers that had delivered their own litters also within the previous 24 hours.

(C) Human islets, isolated postmortem from nondiabetic individuals, were treated with 1 % islet homogenate from old Tg - hIAPP mice, WT control islet homogenates, or old Tg - hIAPP immunodepleted using a cocktail of sequence and conformational antibodies.

Nunez, Kim, Sakamoto and their colleagues carried out a careful series of experiments using both newborn and adult germ - free mice, and samples of gut microbes taken from the feces of 4 - day - old, 12 - day - old and 16 - day - old normal mice.

The researchers used CRISPR / Cas9 gene editing, delivered by a viral vector, to snip part of a gene producing toxic protein aggregates in the brains of 9 - month old mice.

Animals were maintained on the C57BL / 6 background and 8 — 10 - week - old mice of both genders were used if not otherwise indicated.

Lloyd Old, Thierry Boon, and colleagues develop the TNF release assay for mouse systems in which release of TNF by T cells could be used to assess specific T cell recognition, facilitating the cloning of human tumor antigens.

Using whole - cell path clamp, both dSPNs and iSPNs from Q175 6 - month old mice showed elevated membrane resistance and reduced rheobase current, consistent with previous reports of SPN hyperexcitability in mouse models of HD.

Four - to six - week - old female mice were used for tumor injections.

Using a technique called parabiosis, in which the vascular systems of two mice are surgically connected, Villeda's lab had previously discovered that infusing old mice with the blood of younger mice leads to brain rejuvenation, including improvements in learning and memory, while infusions of old blood cause premature brain aging in young mice.

We used 20 - month - old line 85 APPswe / PS1ΔE9 transgenic AD mice (huAPP / PS1) to examine the effect of J147 on the physiological, behavioral and pathological aspects of the disease when administered late in the course of the disease.

Six month - old HET, HOM and WT littermate mice were anesthetized using urethane, and extracellular single unit spikes were recorded in GP or STN.

Another mouse preclinical screening trial targeted the symptomatic stage (between 9 and 11 months)[44] and a study with a plant extract [99] used 23 - to 24 - month - old mice, but to our knowledge no chemically defined AD drug candidate has been tried at the more pathologically advanced stage used in this study.

The success of the treatment in older mice, which corresponded to late adulthood in humans, is particularly important, as this would be the age that would be targeted were this method ever to be used therapeutically in people.

Isolated right and left atria and small mesenteric arteries from wild type and tau deficient (KO)... mice of two age groups (13 and 23 months old) were used to assess cardiovascular phenotypes.

mice of two age groups (13 and 23 months old) were used to assess cardiovascular phenotypes.

To do this, a group of researchers recently used genetic techniques to remove huntingtin from adult mice of different ages, then studied their brains and behavior until old age.

We used 6 - month - old mice, around the earliest age at which we reliably detect behavioral effects of progranulin insufficiency (Filiano et al., 2013).

We were unable to confirm the anti-inflammatory effects of exercise in the frontal cortex using inflammatory gene expression, as the Grn − / − mice used in this study (∼ 8 months when brains were collected) were not old enough to manifest these changes, which become prominent around 1 year of age (Ahmed et al., 2010; Wils et al., 2012).

All mice used were C57BL / 6J, 8 to 10 weeks old and group housed.

Greater running distance in the 2 - to 3 - month - old mice may partially explain these data, but the solo - housed wild - type mice used to test solo - versus group - housing (Fig. 5) ran similar distances as the young mice, yet did not show any increase in brain progranulin levels.

Using a surgical technique, the researchers introduced an experimental demyelinating injury in the spinal cord of an old mouse, creating small areas of myelin loss, and then exposed those areas to cells found the blood of a young mouse.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

We used extracellular field potential recordings to study BST, short - term plasticity (PTP, post-tetanic potentiation; PPF, paired - pulse facilitation) and long - term potentiation (LTP) at the Schaffer collateral - CA1 pyramidal cell synapses in young and old rTg4510 (2 - 3 and 6 - 7 month old) and old APP / PS1 mice (8 - 10 month old).

Female 13 and 52 weeks old tg2576 and APP / PS1 mice were investigated using SmartCube ™ (spontaneous behavior), NeuroCube ™ (Measurement of gait and motor function) and the PhenoCube ™ (social interaction, day / night activity and cognition) Systems.

C.C. Little, the inventor of the modern lab mouse and founder of the Jackson Laboratory, had long used «the age old enmity of woman and the Muridae» as a sales pitch for his model organism.

We explored how aging affects transcriptional dynamics using single - cell RNA sequencing of unstimulated and stimulated naïve and effector memory CD4 (+) T cells from young and old mice from two divergent species.

RNA was prepared from testis of control and IVC - produced 9 - mo - old mice, using the ULTRASPEC total RNA Isolation Reagent Kit (BioTex Laboratories, Inc.) according to the manufacturer's instructions (two animals pooled per sample, with triplicated samples).

For that purpose, 21 - to 23 - month - old mice were treated with daily injections of either recombinant GDF11 (rGDF11, 0.1 mg / kg mouse body weight), a dosing regimen that increases GDF11 levels in old mice toward youthful levels (13), or phosphate - buffered saline (PBS)(vehicle) for 4 weeks, and their blood vessels were subsequently analyzed by using the volumetric assay described above.

Therefore, immunostaining of this molecule was used to visualize vasculature in heterochronic old (Het - O) and isochronic old (Iso - O) mice.

Use natural alternatives such as boric acid for ants and cockroaches and old - fashioned traps for mice and rats.

Nevertheless, mouse studies using controlled diets demonstrate that low protein intake in old age is detrimental.

One such old - school scene, perhaps the most amazing of the film, involved a fight on a machine used in the production of glue traps used for mice, where Jackie and a couple of bad guys try valiantly to fight each other despite parts of their body sticking in glue.

Humor aside, that same event I did finally understand, once I got the schematics put in front of me on paper (you get slower at understanding things when you're older), why someone would use the mouse / keyboard to play a first - person shooter on the PC.

These US history for children color pages make great mouse practice activities for younger children but can also be used with older children beginning to learn how to read.

These US history figures coloring pages make great mouse practice activities for younger children but can also be used with older children beginning to learn how to read.

These Religious Jewish Passover coloring pages make great mouse practice activities for younger children but can also be used with older children beginning to learn how to read.

These Black History coloring pages make great mouse practice activities for younger children but can also be used with older children beginning to learn how to read.