RNA was DNase treated (Roche) and cDNA was reverse transcribed using Transcriptor (Roche) with
oligo dT as the primer.
After DNase treatment, total RNA concentration was quantified and 1 μg total RNA was converted to cDNA using
oligo dT and reverse transcriptase.
Not exact matches
And we can only sell twelve of them
a year because there's not enough
oligos in
the world.
He brought it into Illumina, got it working, licensed it out to
the oligo manufacturers, brought
the price of
oligos down by like 90 % and dramatically increased supply» — which enabled
the now — $ 24 billion - in - market cap Illumina to succeed.
The hope was that antisense oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meag
The hope was that antisense
oligos for everything from cancer to HIV would roll off
the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meag
the pharmaceutical assembly line — but despite
a generation of valiant effort,
the successes on this front have been meag
the successes on
this front have been meager.
(FODMAP is
an acronym for fermentable
oligo - di - monosaccharides and polyols, which is
the name of
the carbohydrate in question.)
About Sisterna... Sisterna B.V. is
the market leader in high - grade sucrose esters and sucrose
oligo esters.
Sometimes lactobacilli need
a bit of help getting hold in
the intestines, and
some practitioners recommend FOS (fructo -
oligo - saccharides) to enhance colonization.
In
a new study, patients who ate rye bread that was low in so - called «FODMAPs» (fermentable
oligo - di - and mono - saccharides and polyols) experienced milder IBS symptoms than patients who ate normal rye bread.
After tinkering with several ingredients, they struck gold with
a mixture of two chemicals,
oligo (e-caprolactone) dimethacrylate and n - butyl acrylate.
A new
Oligo - Miocene marsupial lion from Australia and revision of
the family Thylacoleonidae.
Levels of APOE protein dropped by about half in mice given
the APOE compound as compared with
those that received
the placebo
oligo or saltwater.
The mice that received the APOE antisense oligos had about half as many amyloid plaques as mice given saltwat
The mice that received
the APOE antisense oligos had about half as many amyloid plaques as mice given saltwat
the APOE antisense
oligos had about half as many amyloid plaques as mice given saltwater.
Each molecular group includes tumors from
all grades and categories of astrocytoma, oligodendrocytoma and
oligo - astrocytoma
We put our starting molecule» —
a structure called
oligo - enediyne, composed of three benzene rings linked by carbon atoms — «on
a silver surface, and then induced reactions by heating it.»
I use one of
the fancy pipettes to move
the oligo into
the plasmid tube, and I also add buffer, water, and
an enzyme.
The oligo arrives, and I lose my modern pipetting virginity.
If
all goes well,
the enzyme will cut open
the plasmid, removing
a piece of its DNA and allowing
the oligo to take its place.
My task is to pipette
the oligo from one tiny test tube into
another.
Wagner conducts
the experiment in parallel with me, and his plasmid properly incorporates
the oligo that will guide Cas9 to its target.
After four rounds of selection,
the enriched
oligo pool was sequenced, and
a position weighted matrix was generated for
the 12 bp target site and one flanking residue per side (faded).
MyOPGP data has facilitated
the development of important genomic platforms, such as single nucleotide polymorphism (SNP),
oligo and methylation arrays.
cDNA synthesized by reverse transcription of 1 µg RNA primed with
oligo (
dT) and random 9 - mer primers was used as
the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in
the presence of SYBR ® Green (Stratagene, La Jolla, CA).
cDNA was prepared from total RNA by reverse transcription using standard protocols for
the SuperScript ® III First - Strand Synthesis System (Invitrogen) and
oligo (
dT) priming.
Specifically, reverse transcription of 5 µg total RNA primed with
an oligo (
dT) primer bearing
a T7 promoter was followed by in vitro transcription, generating multiple antisense copies of
each mRNA in
the sample (Ambion, Austin, TX).
Oligo - astheno - teratozoospermia in mice lacking ORP4,
a sterol - binding protein in
the OSBP - related protein family.
Here we utilized BD ™ AbSeq,
a novel protein sequencing approach enabled by
oligo - conjugated antibodies, for simultaneous detection of mRNA and protein in single cells.
We computed Robust Multichip Average expression measures using
the Oligo package in Bioconductor (22).
The antibody - specific oligos were captured, amplified, and sequenced alongside mRNAs in a single workflow on the BD RhapsodyTM single - cell analysis syst
The antibody - specific
oligos were captured, amplified, and sequenced alongside mRNAs in
a single workflow on
the BD RhapsodyTM single - cell analysis syst
the BD RhapsodyTM single - cell analysis system.
gRNAs targeting
every gene in
the human genome for example, can be made easily and cheaply using
oligo library synthesis.
With SapTrap,
the user first designs either
oligos or synthetic DNA for
the desired gRNA target sequence, as well as
the 5» and 3» homology arm repair template (Fig. 1, Step 1).
RNA was isolated from cancer cells using
the RNeasy RNA isolation kit (Qiagen), and complementary DNA was synthesized using
oligo (
dT) primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen).
The primers in the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequenc
The primers in
the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequenc
the KAPA kit target Illumina ® P5 and P7 flow cell adapter
oligo sequences.
Importantly,
this requires
a separate assay: usually,
a custom array with
oligos designed for
each exon in
every panel gene.
The oligo probes are in situ synthesis (Affymetrix 25mer) or spotted (custom ncRNA array 40mer) and immobilized on the ch
The oligo probes are in situ synthesis (Affymetrix 25mer) or spotted (custom ncRNA array 40mer) and immobilized on
the ch
the chip.
Reverse transcription was performed on 1 μg of total RNA with
an oligo (
dT) primer (Roche) and M - MLV RT (Invitrogen).
The data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo prob
The data collected by microarray are based on
the fluorescence signal intensities (analogue data) from known sequenced oligo prob
the fluorescence signal intensities (analogue data) from known sequenced
oligo probes.
A pair of
oligos for
each targeting site (Table S1) were annealed and ligated into linearized pX330 vector for generating gRNA - expressing plasmid.
To further prove
the specificity of
the binding of STAT6 to
the COX - 2 promoter sequence, electrophoretic mobility shift assays (EMSA) were performed utilizing
a 30 bp
oligo from
the COX - 2 promoter sequence encompassing
the STAT6 binding site as
a probe.
The microarrays utilized in the ncRNA Program are oligo - based Affymetrix and in - house custom arra
The microarrays utilized in
the ncRNA Program are oligo - based Affymetrix and in - house custom arra
the ncRNA Program are
oligo - based Affymetrix and in - house custom arrays.
The Glycolytic Versatility of Bacteroides uniformis CECT 7771 and Its Genome Response to
Oligo and Polysaccharides
Orders must be submitted using
the Oligo Ordering Form.
For droplet based data one simple reason for
the heterogeneity could be due to variation in coverage for DNA
oligos on barcoded beads.
FODMAPS is
an acronym for
the unwieldy phrase: fermentable
oligo -, di -, and monosaccharides and polyols (sugar alcohols).
Most likely symptoms of «gluten sensitivity» are actually caused by
an herbicide (weed - killer) and
a group of carbohydrates known as fermentable
oligo - di - monosaccharides and polyols, or FODMAPs for short.
A low - FODMAP (Fermentable,
Oligo -, Di -, Mono - saccharides, and Polyols) diet can also help short term.
And we may even go to
a low FODMAP diet where we cut out
the fermentable carbohydrates — fermentable
oligo - di - po — let's see, fermentable
oligo - di - mono and polysaccharides.
They reiterate that
all of
the following foods can precipate digestive symptoms in IBS patients: dairy, foods which are high in FODMAPS (fermentable
oligo -, di -, monosaccharides, and polyols), high fat and spicy foods, foods with high levels of biogenic amines, i.e. histamine (such as soy), lectins (present in beans), and preservatives, such as benzoic acid and sulfites.
These carbohydrates are part of
the FODMAPs (acronym means: Fermentable
Oligo -, Di -, and Mono - saccharides And Polyols).
Even FODMAP approaches or specific carbohydrate approaches where we cut down
these Fermentable,
Oligo -, Di -, Mono - saccharides and Polyols —
the specific fermentable sugars in
the diet.