Sentences with phrase «oligo sequences»

The primers in the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequences.

Not exact matches

After four rounds of selection, the enriched oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).

For validation, we might be looking at custom arrays or oligo sets in addition to 3730 and 454 sequencing.

Here we utilized BD ™ AbSeq, a novel protein sequencing approach enabled by oligo - conjugated antibodies, for simultaneous detection of mRNA and protein in single cells.

The antibody - specific oligos were captured, amplified, and sequenced alongside mRNAs in a single workflow on the BD RhapsodyTM single - cell analysis system.

With SapTrap, the user first designs either oligos or synthetic DNA for the desired gRNA target sequence, as well as the 5» and 3» homology arm repair template (Fig. 1, Step 1).

The data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo probes.

To further prove the specificity of the binding of STAT6 to the COX - 2 promoter sequence, electrophoretic mobility shift assays (EMSA) were performed utilizing a 30 bp oligo from the COX - 2 promoter sequence encompassing the STAT6 binding site as a probe.