Sentences with phrase «oligonucleotide microarray»

A framework for oligonucleotide microarray preprocessing.

A focused oligonucleotide microarray of 1,178 genes, qRT — PCR of selected transcripts, and western analysis of hypoxia inducible factor - 1α (HIF - 1α) were used to compare retinas from the hypoxic and recovery groups to control animals that were not made hypoxic.

To identify transcriptional patterns that correlate with body mass, we used oligonucleotide microarrays to catalogue gene expression levels in the parametrial or epididymal adipose tissue from two dozen mice whose body mass and adiposity varied due to diet, sex, and mutations in genes affecting energy homeostasis.

Not exact matches

Oligonucleotide probe selection and synthesis — The 1,178 genes comprising the Falk Center for Molecular Therapeutics (FCMT) Rat CNS microarray were compiled from currently available NCBI / EMBL / TIGR rat sequence databases and commercially available central nervous system (CNS) microarrays (Affymetrix, Santa Clara, CA) and provided representation from greater than 90 % of the major gene ontological categories [47].

The oligonucleotide probes were quantitated using a spectrophotometer and immobilized on the microarrays via inclusion of a 5 ′ - amino linker (Glen Research, Sterling, VA) onto each oligonucleotide.

Individual 45 - mer oligonucleotides corresponding to mRNAs of each gene were used as probes, and immobilized on microarray slides, as described below.

Biological and technical replicates for each population were hybridised to NIA Mouse 44K Microarray v2.3 (whole genome 60 mer oligonucleotide probe; manufactured by Agilent Technologies, # 014951)[36].