Sentences with phrase «oligonucleotide probes»

The structure of this enzyme was elucidated by isolating clones from a bovine brain complementary DNA library through the use of oligonucleotide probes derived from partial amino acid sequence.

In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end - labeled oligonucleotide probe hybridized in solution to the amplified beta - globin sequences.

Oligonucleotide probe selection and synthesis — The 1,178 genes comprising the Falk Center for Molecular Therapeutics (FCMT) Rat CNS microarray were compiled from currently available NCBI / EMBL / TIGR rat sequence databases and commercially available central nervous system (CNS) microarrays (Affymetrix, Santa Clara, CA) and provided representation from greater than 90 % of the major gene ontological categories [47].

In hybridization or capture - based target enrichment of samples prior to next - generation sequencing (NGS), pools of biotinylated oligonucleotide probes specific for target regions of interest are hybridized to a sequencing library in solution.

Biological and technical replicates for each population were hybridised to NIA Mouse 44K Microarray v2.3 (whole genome 60 mer oligonucleotide probe; manufactured by Agilent Technologies, # 014951)[36].

A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins.

Four clones were isolated from an adult human brain complementary DNA library with an oligonucleotide probe corresponding to the first 20 amino acids of the beta peptide of brain amyloid from Alzheimer's disease.

The oligonucleotide probes were quantitated using a spectrophotometer and immobilized on the microarrays via inclusion of a 5 ′ - amino linker (Glen Research, Sterling, VA) onto each oligonucleotide.