Comparative Analysis of the Gut Bacterial Community of Four Anastrepha Fruit Flies (Diptera: Tephritidae) Based
on Pyrosequencing — Carmen Ventura — Current Microbiology
Not exact matches
Amplicons were sequenced
on a multiplexed 454 - FLX - Titanium
pyrosequencing run at the Human Genome Sequencing center at Baylor College of Medicine.
One question in analyzing microbial communities by 16S rRNA gene
pyrosequencing centers
on whether the amplification and sequencing methods result in recovery of sequences in proportion to their representation in the original community [24].
These studies, however, were based
on comparing Life Technologies» SOLiD and Illumina's GAII with
pyrosequencing or Sanger sequencing [8], [9], [10], the costs of which are prohibitive for whole - genome sequencing studies in mammals.