Sentences with phrase «on cells in dishes»

The tests took place on cells in dishes.

Experiments on cells in dishes revealed that chronic exposure to corticosterone changed the RNA in these vesicles.

Not exact matches

People who include dishes like carrot soup in their diets on a regular basis also enjoy reduced cholesterol levels, healthy teeth, and gums, wrinkle prevention, the slowing down of aging of the cells in your body, as well as a clean colon.

But the next big question was whether these cells could home in on tumors in lab dishes, and in animals, like neural stem cells.

Chemical biology and drug discovery usually depend on screens of cells in lab dishes, for example.

So far, researchers have mostly turned on genes with CRISPRa in cells growing in lab dishes, says Charles Gersbach, a biomedical engineer at Duke University not involved in the new study.

These techniques include: human tissue created by reprogramming cells from people with the relevant disease (dubbed «patient in a dish»); «body on a chip» devices, where human tissue samples on a silicon chip are linked by a circulating blood substitute; many computer modelling approaches, such as virtual organs, virtual patients and virtual clinical trials; and microdosing studies, where tiny doses of drugs given to volunteers allow scientists to study their metabolism in humans, safely and with unsurpassed accuracy.

Matteo Boretto, the first author on this study, commented that «we were very excited to see that we could not only robustly grow and amplify endometrial tissue in a dish, but that the tiny structures were also able to reproduce normal responses of the endometrium to hormones: oestrogen makes the tissue thicken, progesterone then induces maturation including folding (see picture), and subsequent removal of both hormones mimics the cell shedding of the menstrual period.»

To find out, they put droplets of culture medium that cancer cells had grown in on one side of petri dishes.

One metabolite featured in the study is cholesterol sulfate, which was found to be used up during osteogenesis on a rigid matrix and in turn could be used to convert stem cells into bone - like cells in a dish.

«To date, there has been no systematic means of assessing the fidelity of cellular engineering — to determine how closely cells made in a petri dish approximate natural tissues in the body,» says George Q. Daley, MD, PhD, Director of the Stem Cell Transplantation Program at Boston Children's and senior investigator on both studies.

To test whether the new spheroids were a better mimic for functional dermal papilla cells than those that had been grown in typical dishes, Christiano and her team determined what genes were turned on and off in different sets of dermal papilla cells.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

The trick, Ali's team found, was to embed the stem cells in a gel instead of growing the cells on top of the dish.

The test procedure is performed on an in vitro skin model built at Fraunhofer IGB from human skin cells in special culture dishes.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

Building on previous work from Yuan's lab showing that the activity of RIPK1 could be blocked by a chemical called necrostatin - 1, the research team tested how ALS cells in lab dishes would respond to the same treatment.

On the other hand, cells in a dish lack the critical element of realistic 3 - D structure.

«We've now devised a technology where we can effectively treat the surface of living mouse embryos as a petri dish of cells, and carry out genome wide screens on mouse cells in their native environment in vivo,» says Fuchs.

Kass developed the midstream urine test (still used when you pee in a cup) and set a numerical cutoff for the number of bacteria in normal urine: not more than 100,000 colony - forming units (cell clusters on a culture dish) per milliliter of urine.

Lee, Daugharthy and their colleagues then tested the method to detect the genes skin cells turn on as they multiply and migrate to heal a simulated wound in a petri dish.

Traditionally, cell culture in the lab has been done in petri dishes and on other flat surfaces.

One major roadblock, Miglietta told me, is that most of the work on transcription factors has been done on cells grown in a culture dish.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

On laboratory dishes, these stem cells were found to be havens for viral reproduction, resulting in cell death and / or disruption of cell growth.

However, the usefulness of these studies greatly depends on how accurately these cancer cells grown in a dish represent human tumors.

Benjamin Reubinoff, Tamir Ben - Hur, and their colleagues at Hadassah University Hospital in Jerusalem, Israel, and Monash University in Melbourne, Australia, took a simpler approach, letting the stem cells multiply in a culture dish until they differentiated on their own.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

While past attempts to treat melanoma failed to meet expectations, an international team of researchers are hopeful that a compound they tested on both mice and on human cells in a petri dish takes a positive step toward creating a drug that can kill melanoma cancer cells without harming nearby healthy cells.

After treating the IPS cells in a petri dish to set them on a path to mature into dopaminergic neurons, the cells were grafted into the dopamine - deficient hemispheres of the parkinsonian rats» brains.

Next steps include He's collaboration with Piedmont Atlanta Hospital to retrieve T cells, liver cancer cells and healthy tissue normally removed from patients during surgery, put the mouse receptor genes on these T cells and monitor in a dish both how those cells now fight the tumor and react to healthy human tissue.

Biologists rely on animal models to answer important questions that can't be addressed with cells in a dish.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Acetylcholine is a major transmitter of signals in the brain, and there are several varieties of receptors, or receiver dishes for the signals, on brain cells.

For definitive endoderm differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like cells appeared (Figure 5A), which were positive for definitive endoderm markers such as Foxa2, Sox17 and Gata4.

But when such cells were grown in a laboratory dish, they adhered to the flat surface of the liquid medium they were grown on, spreading out in a thin layer.

«Over the course of time, the cells interact with each other and the collagen to form pieces of artificial heart that beat on their own in a Petri dish,» Elson says.

We came across a compound that had been isolated from a plant species and shown to have effects on blocking the growth of blood vessel cells, both in the culture dish and also in animal models.

This is basically tweaking the molecules: making small chemical changes to the compound in hopes of finding something that could block the growth of blood vessel cells initially in the culture dish without having toxic effects on other cell types.

The work, performed on human brain cells in a dish, paves the way for trying the technique on the brain, with the hope that it could treat a number of genetic conditions.

This is a process in a dish, and we are dealing with a clump of cells which would never go on to develop.

Labelled outer segments were washed, resuspended in human foetal RPE medium and seeded onto iPS - RPE cells cultured on gelatin - coated 35 mm dishes.

In 2010, Srivastava and a former postdoctoral fellow, Masaki Ieda, PhD, expanded on that breakthrough with direct reprogramming in a dish, which allows scientists to transform one adult cell type into another without first reverting back to a stem cell statIn 2010, Srivastava and a former postdoctoral fellow, Masaki Ieda, PhD, expanded on that breakthrough with direct reprogramming in a dish, which allows scientists to transform one adult cell type into another without first reverting back to a stem cell statin a dish, which allows scientists to transform one adult cell type into another without first reverting back to a stem cell state.

No differences in the expression of the pluripotent marker Oct4 (Fig. 1C — D) were found between cells cultured in 96 - well plates and hESCs routinely passaged in medium conditioned on MEF in larger dishes (35 - mm or 60 - mm dishes).

And then dripped their blood on human breast cancer cells in a petri dish over the next six hours.

As well as being on of the best family of vegetables to slow the proliferation of stomach cancer cell growth in a petri dish, cruciferous vegetables have many other great health benefits and you can check out more videos here

This makes sense, given new research showing, for example, that apple peels have potent antioxidant and antiproliferative effects on human cancer cells in a Petri dish.

Scientists at South Dakota State University tested the effects of this substance, known as PEITC, on human cancer stem cells in a Petri dish.

There are a number of specific compounds in plant foods that look promising, but that's based on in vitro studies like this, where they basically just drip some plant compound on bone cells in a petri dish and see a boost in bone builder cells, or a drop in bone eater cells.