Most of the suspect peptides had no effect
on the cultured cells, but seven viral peptides and one peptide from a bacterium made the T cells start dividing.
Potti and his colleagues began by testing chemotherapy drugs
on cultured cell lines from human tumors, such as from the lung, breast, or ovary.
Execution of DNA extraction using the Promega genomic DNA purification system
on cultured cell samples.
Not exact matches
Based
on a 3D image such as an MRI scan, Aspect's machine builds relatively complex organic structures out of a «hydrogel» embedded within
cells taken from the body and grown in a
cell culture.
Bethencourt and Shigeta figured they could use
cell cultures to grow meat without slaughtering any cows or chickens — but why rely
on those animals at all?
Rather than obtaining meat from animals raised
on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal
cells and replicating them in a
culture outside of the animal.
I was inspired by the vulnerability of that
culture; the way people spill their life out over their
cell phones while you're sitting next to them
on the subway.
If Christians believe that the church and the worship it offers to God ought in some ways to counter the norms and practices of the surrounding
culture, then what does it mean that after spending so much of our time each week in front of computer monitors,
cell phones, and sports bar TVs, we come to church
on Sunday and happily position ourselves in front of the biggest screen of all?
Rather than obtaining meat from animals raised
on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal
cells and replicating them in a
culture outside of the animal.
Ohly offers a wide range of products such as yeast extracts, inactive dry yeast, special vitamin yeast, yeast
cell wall derivatives, medical yeast and autolysed yeast, based
on bakers and / or Torula yeast, as well as wine yeast, specialty powders and starter
cultures.
Culturing stem
cells by day, and audiences in dingy stand - up clubs by night, it wasn't until Helen Pilcher left the lab that she started basing her comedy
on science.
While other papers have examined these mutations using expensive and time - consuming experiments
on live ferrets and laboratory
cell cultures, Deem and Melia Bonomo used the pEpitope method to rapidly calculate how much the egg - passage mutations would decrease vaccine efficacy in humans.
Scientists are forced either to experiment
on whole animals, which is expensive, raises ethical issues and may not predict effects in humans, or to perform tests
on microscopic human
cells found in tissue
cultures, which have been altered to live forever and bear little relation to actual living, breathing people.
Cyclic adenosine monophosphate (cAMP)- dependent protein kinase, labeled with fluorescein and rhodamine
on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in
culture or in intact
cell clusters.
For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they
cultured chondrocyte
cells in a dog bone - shaped mold with loops
on either end.
The team tested the technique
on cells in
culture that mimic the transformation of unspecialised white blood
cells into full immune
cells.
To understand what was going
on, Heber - Katz, started examining the
cells filling in the holes in
culture.
Still, only a few dozen labs in the world are certified as BSL4 facilities; some are very small and only work
on diagnostics, or
cell cultures, or a single species of animals.
The Wyss team believes the ability of the human gut -
on - a-chip to
culture the microbiome with human gut
cells also holds promise for the field of precision medicine, where a patient's own
cells and gut microbiota could one day be
cultured inside a gut -
on - a-chip for testing different therapies and identifying an individualized treatment strategy.
With our human gut -
on - a-chip, we can not only
culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune
cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»
One week, the pair went to a conference to present a poster
on the aging of human
cells in
culture.
A team spearheaded by Dr. Markus Helfer and Prof. Dr. Ruth Brack - Werner from the Institute of Virology and Prof. Dr. Philippe Schmitt - Kopplin from the Analytical BioGeoChemistry research unit (BGC) performed a detailed investigation of the effects of PS extracts
on HIV - 1 infection of
cultured cells.
The centre will be structured around six technical platforms focusing
on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis, flow cytometry, stem
cell culture, and gene - expression analysis.
Some of the leading non-animal tests are conducted
on cell cultures, human and animal corneas from eye banks, corneal tissue
cultures, and frozen corneas supplied by hospitals.
Experiments and observations may be conducted
on humans, animals,
cell cultures, chemical platforms, etc..
One would
culture thin sheets of meat, seeded by
cells from a living animal,
on a reusable polymer scaffold; the other would grow meat
on small edible beads that stretch with changes in temperature.
One of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment
on Scott's abilities in the area of «Approaches to Developing Serum - Free Media Formulations for Mammalian
Cells in
Culture.»
Previously, high - resolution live imaging has been done with
cells cultured on glass slides, which flattens samples.
By analyzing chemical changes of the IRS - 2 protein in immortalized
cultures of human white blood
cells, it determined that IRS - 2 appeared in two different forms — «
on,» which allows the signal to pass through, and «off,» which stops the signal from activating the
cells into M2 macrophages.
In experiments
on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gab2.
What he showed is if he took the fluids from
cultures of
cells that lit up and squirted them
on cells that were at a low
cell density, they all turned
on light.
Human nasal epithelial
cells,
cultured on a microchip, react to air pollutants just like they would in the upper airway.
Genomic RNA editing and its impact
on Ebola virus adaptation during serial passages in
cell culture and infection of guinea pigs
«It's possible that the
culture conditions are causing increased stress
on the
cells, resulting in
cell death.»
To find out, they put droplets of
culture medium that cancer
cells had grown in
on one side of petri dishes.
If a breast cancer line had silently contaminated Klonisch's
cell culture early
on, it would have been subject to the usual technique for immortalizing a normal
cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).
The team's further experiments
on cultured heart
cells confirmed that GDF - 11 stops the thickening growth seen with age.
Hayashi then drew
on other research showing that one key regulatory molecule (activin A) and a growth factor (basic fibroblast growth factor) could convert
cultured early embryonic stem
cells into
cells akin to epiblasts.
In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial
cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial
cells cultured on bovine collagen.
The ability to automate morphology assays
on 3D
cell cultures is a powerful tool, but some scientists are now pushing the technology even further, with algorithms that can classify structures in living animals and clinical specimens.
In addition to emphasizing the recruitment of graduate students, American universities have a number of
on - campus service facilities such as modern research labs, well - stocked reagent rooms, and
cell culture facilities.
Several researchers, including Cridland, have consistently failed to find any effects of EMFs
on their
cell cultures.
Although no tests have yet been done
on real wounds, experiments
on cultured human skin
cells have been encouraging, he says.
We were able to use pharmaceuticals to induce
cell self - eating effects and stimulate dedifferentiation though not as effectively as 3D
culture, so we need to do more work
on this.
The implications were hardly lost
on the Bethesda crowd: If the virus was transmitted in
cell cultures in Ruscetti's lab, it could also be contaminating the nation's blood supply as a result of blood donations from unknowingly infected donors.
«In a given week I'll do
cell culture, protein chemistry, molecular biology, microscopy, and structural biology
on the computer,» adds Luhrs.
Barnes's team went
on to show last year that soya extracts could also inhibit the growth of prostate cancer
cells in laboratory tissue
cultures.
After successfully imaging
cultured cells, the researchers tested their microendoscope
on anesthetized mice.
I found that
culturing cardiac valve
cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the
cells» cytoskeletal structure.
The journal's home page explains that translational medicine «builds
on basic research advances — studies of biological processes using
cell cultures, for example, or animal models — and uses them to develop new therapies or medical procedures.»