Sentences with phrase «on embryonic mice»

In laboratory tests on embryonic mice engineered to not express myomerger in skeletal muscle, the animals did not develop enough muscle fiber to live.

Not exact matches

MTHFR + / + or + / - pregnant mice on a control diet or folic acid - supplemented diet (20-fold higher than the recommended intake) were examined for embryonic loss, delay, and defects.

«Our research on mice and rats shows that these chemicals affect the embryonic development of these animals.

Here (from left to right), they're shown on the embryonic skin of a mouse, a snake, a chicken and a Nile crocodile.

Early in embryonic development, both mouse and human placentas rely on the same set of ancient cell - growth genes.

Manuel Eguren has analysed the biological consequences of Cdh1 elimination in rapidly dividing cells, as part of his doctoral research project in Malumbres's group; he focused on progenitors from the nervous system during embryonic development in mice.

Mitchell and her colleagues eliminated this possibility when they deleted these nearby regions in the genome of mice and found there was no impact on the gene's ability to be turned on in embryonic stem cells.

«We studied how the Sox2 gene is turned on in mice, and found the region of the genome that is needed to turn the gene on in embryonic stem cells,» said Professor Jennifer Mitchell of U of T's Department of Cell and Systems Biology, lead invesigator of a study published in the December 15 issue of Genes & Development.

They first inserted each enhancer into embryonic mice to learn whether it really did turn genes on.

The scientists already knew how to encourage mouse embryonic stem cells to express nestin, and they wondered if they could coax their nestin - positive cells to take on more characteristics of pancreas cells.

An ambitious plan to map the genome's regulatory elements will focus first on mouse embryonic stem cells.

They have learned how to turn on and off essential genes and how to transform embryonic stem cells into retinal cells, which can be transplanted into mice to restore vision.

The researchers took skin cells from the tails of sickle cell mice and inserted copies of four genes that made the cells take on the characteristics of embryonic stem cells.

Current efforts are focused on defining the molecular mechanism underlying the embryonic lethal phenotype of ADAR1 null mutant mice and identification of the dsRNA (s), which triggers the MDA5 - MAVS - IFN pathway.

A transient embryonic («primitive») phase of hematopoiesis, beginning on E7.5 in the mouse embryo, is followed by the definitive («adult») phase, which begins on E9.5 in the liver of the developing mouse embryo.

The team spent over a year optimising their techniques using mouse embryos and human embryonic stem cells before starting work on human embryos.

In this study, the team delved deep into the nucleus of cells belonging to mouse and zebrafish embryos — two important animal models of embryonic development — in order to determine how the Dll4 gene is turned on.

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

By introducing the genes for four factors that turn genes on and off, he induced the skin cells of adult mice to become like embryonic stem cells, which he called induced pluripotent stem (iPS) cells.

We also found that the loss of DJ - 1 had no effect on the weight phenotype of the Polg mutator mice, that none of the nine possible combinations of DJ - 1 and Polg genotypes were embryonic lethal, and that there was not increased astrogliosis in the Polg mutator DJ -1-deficient mouse SNpc.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

It is based on the definitive books of mouse embryonic development by Theiler (1989) and Kaufman (1992) yet extends these studies by creating a series of three dimensional computer models of mouse embryos at successive stages of development with defined anatomical domains linked by a stage - by - stage ontology of anatomical names.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

Here we present a morphological, topological and metric analysis of the heart and arteries of mouse embryos harvested on embryonic day (E) 14.5, based on digital volume data of whole embryos analysed by high - resolution episcopic microscopy (HREM).

In this experiment, Capecchi used different sets of mice to turn on the mutation in three developmental stages — early embryonic, prenatal and postnatal.