Sentences with phrase «on human cell lines»

Our investigations on human cell lines in the laboratory have shown that synthetic cannabinoids, in the high concentrations found in cells in the oral cavity or in the lungs, for example, are likely to trigger damage to the DNA that may have significant consequences for the consumers of such substances.

Not exact matches

In August of last year, President Bush approved the use of federal funds to support research on a limited number of existing human embryonic stem cell lines.

Modern psychosomatic medicine has made some progress in analyzing along these lines; for example, it seems quite possible that the emotional tone of my soul may directly alter the patterns of physical feeling in my stomach.4 Still, we should not suppose too quickly that the aims of a human personality have any very effective direct influence on the molecules of body cells, other than those in the brain.

To create the effect of tobacco smoke on cells, Vaz, Baylin and their colleagues began their studies with human bronchial cells, which line the airways of the lungs, and grew them in a laboratory.

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Congressional supporters of stem cell research have re-introduced legislation to codify President Barack Obama's 2009 executive order lifting restrictions on the number of human embryonic stem cell lines available to federally funded researchers.

«This model was trained on genetic data from human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greene said.

Potti and his colleagues began by testing chemotherapy drugs on cultured cell lines from human tumors, such as from the lung, breast, or ovary.

Another problem is that in its July 2009 Guidelines on Human Stem Cell Research, NIH spelled out specific requirements about embryo donation for newly derived lines, says Pilar Ossorio, a legal scholar who studies research ethics at the University of Wisconsin Law School.

In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.

Last June Ingber's team reported that it had placed human lung lining cells and human capillary cells on either side of a porous, flexible polymer membrane.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

Her group will focus on the biogenesis and homeostasis of mitochondrial proteins and add mammalian and human cell lines to current yeasts.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

MERS CoV (CoV stands for coronavirus) has on its surface an array of spike - shaped proteins that bind to host cells — specifically to receptor proteins called DPP4 on the surface of cells that line human airways.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

In addition to the afatinib - resistant NSCLC cells, the researchers tested the neratinib and valproic acid combination on cell lines derived from human pancreatic and ovarian cancers containing K - Ras mutations and N - Ras mutations, respectively.

The bill was put forth to loosen the restrictions Bush placed on human embryonic stem cell research on August 9, 2001, when he banned federal funding for work with any stem cell line created after that date.

While conservatives in Congress took turns echoing George W. Bush's opposition to destroying human embryos for research, Lensch's colleague Paul Lerou stepped into a small room behind a heavy black curtain to check up on a line of nonpresidential embryonic stem cells.

The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.

To test their hypothesis, the researchers carried out experiments on human melanoma cells, a line of cancer cells they chose for their ability to grow easily and quickly.

The scientists used the new indexing method on several human cell lines and from a mouse brain to reveal the methylome of 3,282 single cells.

Alternative cell lines, such as induced pluripotent stem cells generated from patient skin cells, offer a more accurate window on human biology, he says.

In a statement put on the Web this morning, NIH reported that all 64 lines «show characteristics of stem cell morphology» and have undergone several population doublings, and most of them have demonstrated all the protein markers «known to be associated with human embryonic stem cells.»

Yet, «these studies using HeLa cells had to rely on information from the «reference» sequence produced by the Human Genome Project, even though there was evidence that the genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

The researchers tested honokiol on cell lines derived from human cancers of the oral cavity, larynx, tongue, and pharynx.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

The long - awaited registry of human embryonic stem cell lines approved by the National Institutes of Health (NIH) went up on the Web this week.

In the PNAS paper, the scientists demonstrated their method on two genomics problems, the role of gene enhancers in the fruit fly embryo and alternative splicing in a human - derived cell line.

Paper on creation of stem cell line from human blastocyst contains fabricated data, university committee concludes

But a computer game that puts those kids on the front line of a battlefield inside the human body — where cancer cells and chemotherapy drugs are slogging it out for supremacy — just might persuade them that it's important to keep taking the drugs.

To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.

Now, however, a team of researchers led by Yu - Hua Tseng, Ph.D., Investigator in the Section on Integrative Physiology and Metabolism at Joslin Diabetes Center and an Associate Professor of Medicine at Harvard Medical School, has created cell lines of human brown and white fat precursor cells that will help investigators to pick apart the factors that drive the development and activity of each type of cell.

For while the new NIH guidelines explicitly permit funding for research on stem cell lines in which human embryos have already been destroyed, they also explicitly forbid funding for research on stem cell lines that have been produced by SCNT (see section V. part B).

To do so, they started with a human embryonic stem cell line, which they chemically nudged to become cells that form what's known as the primitive streak on the hollow ball of cells of the early embryo.

In cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lining.

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

To develop protocols for production of transplantable mesDA and striatal GABAergic progenitors from human ES and NS cell lines, building on recent developments in stem cell biology;

Peter and colleagues focused their initial studies on a standard panel, known as NCI60, of 60 human tumor cell lines that can genetically be divided into two large groups, which they called superclusters 1 and 2.

Panel A. Staining for HNF4α was performed on enhanced hiPS - HEP cells from three different hiPS cell lines (C12, C18, and C22) on Day 12 post-thawing and cryopreserved human primary hepatocytes (hphep cells) on Day 1 post-thawing.

\ n \ nIn other words this is a political attempt, based on a technicality, to advance science in the field by getting the US Administration to greatly expand the number of human ES - cell lines that can be used under federal funding.

All the hES and human induced pluripotent stem cell lines were confirmed to be genetically stable and pluripotent in in vivo and in vitro assays after extensive culturing on the new substrata.

It has not been grown in a T - cell line, only on human PBMCs

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRIIb on the surface.

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRI on the surface.

This is a derivative of the TZM - bl cell line, engineered by lentiviral transduction to stably express human FcγRIIIa on the surface.

The DNA in our cells, which contains the genetic instructions that make us human, is passed on from parent to child along an unending line of generations.

Building on that project, the researchers have now designed a machine that smokes cigarettes in a very human - like manner, and hooked it up to organs - on - chips lined by human lung small airway cells so the smoke interacts with these cells in a realistic way.

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.