Sentences with phrase «on laminin»

Human fetal NSCs (ReNCell VM, Chemicon) were cultured on laminin - coated dishes in ReNcell maintenance medium (Chemicon) in the presence of basic fibroblast growth factor 2 (bFGF2), following the manufacturer's instructions.

The human NSCs have a typical, undifferentiated neural stem cell morphology when expanding as monolayers on laminin - coated plates (Fig. 1A).

ES derived hepatocyte differentiation on laminins represent significant improvement of metabolic activity and functional organisation

Not exact matches

As expected, additional experiments by Abe showed that the F1 - actin flow was dependent on L1 - CAM interactions with laminin.

The new study by the Inagaki lab showed that neurons will produce four times more traction force when placed on adhesive substrates coated with laminin than those without laminin, assuring the axons reach their final destination.

Cells were resuspended in HEPES buffer containing the following (in mm): 136 NaCl, 5.6 KCl, 1 MgCl2, 2.2 CaCl2, 11 glucose, 10 HEPES; pH adjusted to 7.4 with NaOH before plating on poly - d - lysine (0.02 mg / ml; BD Bioscience) and laminin (0.02 mg / ml; Sigma - Aldrich)- coated coverslips.

Stem cell culturing of human ES and iPS cells on recombinant laminin matrices is easy and efficient.

Buy human recombinant laminins from BioLamina - culture stem cells, differentiate to neurons, RPE cells, cardiomyocytes and other cells on their specific laminin isoform.

BioLamina believes in sharing knowledge - here you will find information available on all the laminin cell culture applications.

Here, we show hES cells plated as single cells on top of recombinant laminin - 521, growing from day 0 to day 5 when they are ready to be split again.

Here, Kate presents her work on that show that culture of human ES cells on human recombinant laminin - 521 and laminin - 111 substrates significantly improve hepatocyte differentiation, maturation, function and stabilization of phenotype compared to Matrigel cultured cells.

LN521 based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.

Cells were plated either on glass coverslips (40,000 cells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin alone.

Cells were grown on polyornithine and laminin coated dishes and passaged 1:2 with PBS + + (PBS with 1 % BSA).

and cultured in 5 % O2 / 7 % CO2 in Bottenstein - Sato F12 medium with 10 ng / ml human recombinant basic fibroblast growth factor (Peprotech) on a substrate of 1 µg / cm2 fibronectin (Chemicon) and 0.5 µg / cm2 laminin (Invitrogen).

Morphogenetic effects of soluble laminin - 5 on cultured epithelial cells and tissue explants.

Laminin - 511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES / iPS) stem cells, as it binds to integrin on cell surfaces.