Sentences with phrase «on lysed»

Additionally, most often detection is carried out on lysed cell samples, so a significant amount of information is lost.

Not exact matches

Cells were lysed on ice using lysis buffer (10 mM Tris - HCl pH 7.4, 150 mM NaCl, 1 % Triton X-100, protease inhibitor mix G (Serva) and 2 mM Na3VO4).

Cultured cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 μl RIPA per 1 × 106 cells; 50 mM Tris / HCl; 150 mM NaCl, 1 % Triton X-100, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, 1 μg / ml leupeptin and 1 μg / ml pepstatin) for 1 h on ice, and insoluble debris was removed by centrifugation.

Cells were washed with ice - cold PBS, lysed for 15 minutes on ice with M - PER protein extraction reagent (Pierce), scraped, and spun at 14,000 rpm for 10 minutes at 4 °C.

Supplies Serratia peptidase enzyme to help protect sensitive GI tissues from irritation and lysozyme for its unique lysing action on bacterial and yeast cell walls

A view from the streets and the chancelleries: Lyse Doucet attempts a perspective on the conflict of our century