Sentences with phrase «on microarrays»

• Organized workflow directed towards bioassay design using Protein and DNA signatures on microarrays to test efficacy of detecting bio-threats from samples that included challenged and naturally occurring outbreaks.

Profiling the autoantibody repertoire in plasma, serum or CSF by screening on microarrays of randomly selected antigens.

We also describe a method for the bulk testing of the hybridization characteristics of chromosome - specific clones spotted on microarrays by use of DNA amplified from flow - sorted chromosomes.

We obtained polymorphism and divergence data from six individuals for each of the rabbit subspecies using Illumina sequencing of genomic regions enriched by DNA hybridization on microarrays [60].

Chromosomal position of the genomic regions targeted with the hybridization capture on microarrays.

Geographical location of the populations sampled for the transcriptome (red circles) and DNA hybridization on microarrays datasets (black circles).

The oligonucleotide probes were quantitated using a spectrophotometer and immobilized on the microarrays via inclusion of a 5 ′ - amino linker (Glen Research, Sterling, VA) onto each oligonucleotide.

Here we show that hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ~ 99.8 % microbial DNA.

They work on microarray - based gene sensors for analyzing nucleic acids quickly and cheaply.

He's excited to see a clear readout for a process that would otherwise require decoding a complex pattern on a microarray, and he thinks the idea of simple alphanumeric display could be applied to other types of molecular logic as well.

«The genetic content that goes on a microarray is as important as the technology itself,» he says.

Only Z - score analysis, a method for assessing the statistical significance of individual Virochip probes, yielded a credible viral signature on the microarray.

Using the GOMiner algorithm, two independent category structures (biologic process and molecular function) based on the 1178 rat genes represented on the microarray (of which 923 currently carry GO annotations) were initially constructed and used as Query gene files.

Individual 45 - mer oligonucleotides corresponding to mRNAs of each gene were used as probes, and immobilized on microarray slides, as described below.

Other proteins can't be put on a microarray at all because they are part of a cell membrane and need to be attached to their membranes to function.

In addition, for genes represented multiple times on the microarray, we selected the transcript with the strongest average signal as representative for the respective gene.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

Eager to sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking for molecular geneticists working on different stem cell systems; scientists with genomics bents like proteomics, microarray analysis, and bioinformatics; and a couple of clinician scientists.

This study, published in the journal Microarrays, shows that lack of SOST in the bone microenvironment promotes the expression of many genes associated with cell migration and / or invasion, including long non-coding RNA MALAT1 in prostate cancer, suggesting that SOST has an inhibitory effect on prostate cancer invasion.

On a study funded by the National Science Foundation and Agilent Technologies, researchers employed spatial light interference microscopy (SLIM), a label - free method, to perform localized measurements of light scattering in prostatectomy tissue microarrays.

He worked there for 3 years, continuing his research on telomeres and pioneering the microarray techniques that form the basis of his international reputation.

DNA microarrays consist of thousands of spots contained on a single 1 x 3 inch glass slide, each spot representing a different gene.

We ship over 1500 microarrays each month and provide training and support to over 280 academic labs on five continents.

Production Group: Focuses its efforts on producing microarrays and refining manufacturing techniques.

The Microarray Centre's researchers design and distribute arrays and provide training and support to over 260 labs on five continents.

Traditionally, gene function was determined one gene at a time, but today's microarray technology enables scientists to monitor the expression of tens of thousands of genes on a single glass slide.

This approach combines the molecular recognition that occurs on the surface of a microarray and the pattern recognition traditionally done by an electronic computer into a single step.

Subjecting N. gonorrhoeae to the phenotypic microarray screening method for the first time, Sikora's team focused on seven proteins from the bacteria's cell envelope, which consists of the outer membrane, the cell wall and the inner membrane.

One of the techniques she uses is SNP microarrays, which enable researchers to screen a genetic sample for many SNPs at once on specially designed DNA «chips».

Some research teams over the past five years have used microarray or gene - chip technology to compare genomes and quickly scan them for variations of copy numbers on each chromosome.

He is on the Board of Directors of the Cancer Cytogenomics Microarray Consortium and reviews manuscripts for many top journals in his field as well as having over 150 peer - reviewed publications.

Furthermore, they are undertaking an additional validation measure on several SNPs via the TaqMan ® assay, a non — microarray - based genotyping method.

Using microarrays that enabled them to examine 100,000 sites on the genome, they looked at the cells» nuclear DNA, mitochondrial DNA, and methylation patterns (which give clues to whether a gene has been turned on or off).

Epifluorescence image of HeLa S3 cells grown on top of a two - molecule microarray.

Target preparation and microarray hybridization — Total RNA was extracted from retinal samples using RNeasy Lipid Tissue Mini Kit (Qiagen) and was the substrate for amplification and labeling using a procedure based on the Eberwine protocol [49].

If, for instance, a researcher finds that dozens of genes in a microarray are differentially expressed on a high - fat purified ingredient diet compared to a low - fat GB diet, it is tempting to conclude that gene expression was altered due to the differences in fat levels between the diets.

To assess the expression of Tie2 and other Ang / Tie signalling molecules on human pericytes, a microarray - based expression profiling of human brain pericytes (BP), placenta pericytes, pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC) was performed.

The intensity of the emitted fluorescence from a target spot on the array was detected using an Agilent G2565BA microarray scanner.

The results, which will appear in the Journal of Microbiological Methods, make it clear that the methods used to isolate RNA can have a significant impact on the variability, trend, and accuracy of microarray data.

This indicates that the antibody is monospecfic for p53 when analyzed on a denatured microarray.

When evaluated on a on a denatured microarray (top), the difference between Rank 1 and Rank 2 is 67.63 (standard deviations).

Research on vaccine biomarkers, including in - depth comparative analysis of data from different platforms, large - scale RNA sequencing, harmonisation of standard operating procedures (SOPs) for sample, microarray and data analysis, as well as transcriptome mapping (more than 1400 samples analysed).

Using the HuProt microarray array, the binding of fluorescently labeled or biotinylated sample proteins to proteins on the HuProt human proteome microarray — the largest collection of human proteins in the world — can be analyzed simultaneously.

However, when the antibody is evaluated on a denatured microarray (above) it is no longer specific for p53, and is, in fact, monospecific for C20orf77 as the S - Score (between rank # 1 and rank # 2) is 9.55.

Dr. Li's research interests focus on machine learning, bioinformatics, and statistical data mining in large scale data in biomedical research, such as next generation sequencing data (whole genome sequencing, RNA - seq, microarray data), in the file.

The data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo probes.

However, our receptor gene data is only moderately similar to nanoCAGE (Spearman correlation = 0.38; Figure 6B) and the abundance estimates of the OR and VR genes represented on the Illumina microarrays (Spearman correlation = 0.54 for OR and 0.29 for VR genes; Figure S6).

Waters, who is director of biological sciences at PNNL, was invited to be on the committee because of her strong expertise in microarray and omics data analysis.

Hu C et al. (2015) Autoantibody Profiling on Human Proteome Microarray for Biomarker Discovery in Cerebrospinal Fluid and Sera of Neuropsychiatric Lupus.

The discovery of XMRV was accomplished using a broad - spectrum microarray assay (ViroChip) designed to detect all known viruses as well as novel viruses on the basis of sequence homology [1], [2], [3].