Sentences with phrase «on subcellular»

«The proteomics field is now entering a third generation phase where large - scale protein analysis will provide unique quantifiable data on a subcellular level «says one of the participants in the EU funded PROSPECTS project Dr. Emma Lundberg from the Science for Life Laboratory Stockholm.

In order to localize the whole human proteome on a subcellular level in one specific cell line a third cell line, U-2 OS, is always chosen.

Confocal microscopy of convertases and known disease - related human convertase mutants in cells and tissues will also be used to shed light on subcellular targeting mechanisms.

a publically available portal for researchers in life sciences and provides access to protein profiling data in 46 normal tissues, 20 cancer tissues, 46 cell lines and on a subcellular level.

In the April issue of Experimental Biology and Medicine a multidisciplinary research team led by Drs. Rex Gaskins and Paul Kenis in the Institute of Genomic Biology (IGB) on the campus of the University of Illinois Urbana - Champaign describe their recent work on subcellular redox homeostasis.

Despite choloroquine's smashing success, figuring out how it worked on a subcellular level took a lot longer to unravel.

To address that, Tao and his colleagues focused on the subcellular vesicles released by the tumors.

Not exact matches

The third and newest approach to liquid biopsies, exosome screening, relies on circulating subcellular vesicles.

For them, that exercise might seem equivalent to pondering whether those individual subcellular constituents are alive on their own.

«The cytoskeleton system is comprised of a host of interacting subcellular structures and proteins, and our technique will enable research on the interactions between these different targets with unprecedented number of color channels and spatial resolution,» he said.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

«There must be some genes that are necessary and possibly also sufficient to guide the chandelier cell axons to this subcellular target,» said Andre Steineke, Ph.D., Postdoctoral Researcher and lead author on the study.

We demonstrated the technique on 20 different biological processes spanning four orders of magnitude in space and time, including the binding kinetics of single Sox2 transcription factor molecules, 3D superresolution photoactivated localization microscopy of nuclear lamins, dynamic organelle rearrangements and 3D tracking of microtubule plus ends during mitosis, neutrophil motility in a collagen mesh, and subcellular protein localization and dynamics during embryogenesis in Caenorhabditis elegans and Drosophila melanogaster.

The American Journal of Physiology - Heart and Circulatory Physiology focuses on the physiology of the heart, blood vessels and lymphatics, including experimental and theoretical studies of cardiovascular function at all levels of organization ranging from the intact animal to the cellular, subcellular and molecular levels.

To avoid such perturbations, researchers have developed a computer program that can distinguish between cell types and identify subcellular structures, among other features — all without the fluorescent probes our human eyes rely on.

The subcellular localization of the Niemann - Pick Type C proteins depends on the adaptor complex AP - 3.

We invite original innovative research on the rational engineering of plant systems at all levels, including proteins, protein complexes, sensors, metabolic and signalling pathways, microcompartments such as carboxysomes, subcellular compartments, cell types or tissues, as well as the engineering of novel plant hybrid species and the interactions of plants with viruses, bacteria, and other organisms.

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent proteins for subcellular environment.

He works with a group on the Cell Atlas, a high resolution subcellular atlas showing the spatial distribution of proteins within cells.

The article gives an overview on techniques that are used to locate proteins within cells and Emma Lundberg shares her experiences from her work with the Subcellular Protein Atlas within the Human Protein Atlas, HPA.

This provides spatial information on protein expression patterns to define the subcellular localization to cellular organelles and structures.

And nanoscale imaging of the brain promises to open up a brave new world of questions about brain function and structure on a cellular and subcellular level.

The HPA evidence is calculated based on the manual curation of Western blot, tissue profiling and subcellular location as well as transcript profiling using RNA - seq.

Based on the resource of more than 47,000 antibodies and a panel of > 20 different human cell lines, this facility has a unique position to investigate subcellular spatial proteomics for human and rodent biology.

With the subcellular location restricting a protein's possible function, this method should be a useful tool for the systematic analysis of genome data and is available via a server on the world wide web.

The successful candidate will receive extensive training on proteomics - based discovery (secretome proteomics to identify cytokines important in different children's cancers, protein expression profiling of subcellular fractions and organelles, and interactome proteomics to identify novel protein — protein interactions) and follow - up of proteomic findings (molecular cell biological characterization of protein functions and signaling).