Sentences with phrase «one's protein of interest»

I am also interested in studying small molecule labeling of proteins of interests, which can also prove to be a scientific approach to elucidate protein dynamics in normal as well as cancer cells.

For example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green and blue or cyan and yellow moxFPs.

The scientists attached each half of the luciferase to two proteins of interest so that if the proteins associate for any period of time, luciferase's two halves are brought together and emit light.

The strict correlation between the fluorescent protein of interest and the reference protein -LRB-

In addition to analysis using the full HuProt microarray, CDI can custom print microarrays containing up to 14 separate subarrays - each containing only proteins of interest as determined during the discovery phase.

TRAP can also be easily unlinked from the tag with a simple biochemical treatment, allowing researchers to piece out the coworker from their original protein of interest.

We encourage investigators to characterize FP - fusion proteins functionally relative to the untagged protein of interest.

We can then take proteins of interest, change them in some specific way, and then put them back into a cell to create a sort of modified synapse.

Observing a bright signal for the first time after cloning an FP fusion with your favorite protein of interest is exciting and rewarding.

By breeding NOD mice to produce a certain human protein of interest, e.g. CD3, researchers can test the effects of the antibody targeting this protein and refine the approach to make it more effective.

The DT40 evolution platform also supports using gene conversion competent cells and homologous donor sequences as «pseudo genes» to evolve proteins of interest [24]--[25].

Then, if we treat the cell with chemo, a stress agent, or a DNA - damaging agent, we can see what happens to all the proteins that our single protein of interest comes in contact with.

RHPs are composed of four types of monomer subunits, each with chemical properties designed to interact with chemical patches on the surface of proteins of interest.

The strict correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental conditions.

Matt Stanton, Moderna's head of chemistry, says the company's innovation can use exogenous, synthesized mRNA to create any protein of interest in targeted cell types or tissues.

Our mRNA technology acts as cellular software, directing the body's cellular machinery to produce nearly any protein of interest.

When CTB was not part of the fused protein, they did not see this expression, suggesting that their carrier protein, the crossing guard, was an essential part of delivering their protein of interest.

Researchers then connect the inactive halves of DAM to proteins of interest they suspect bind near each other, or that even bind to each other in order to bind DNA.

We then interpret this as evidence that the two proteins of interest are physically interacting with each other — a molecular signaling event.

The team then performed a detailed analysis on three proteins of interest, which in normal conditions form a complex that links amino acids to transfer RNAs (tRNAs).

The adapter works like a tiny trailer hitch, enabling the drug to tow the cell's protein - degradation machinery directly to the protein of interest.

«We first remove all communication lines associated with a protein of interest and add back a single line.

These networks have traditionally been studied by removing a protein of interest through genetic engineering and observing whether the removal destroys the function of interest or not.

For instance, with human growth hormone and human insulin, the first ones to be made by a recombinant DNA, you take one gene, put it into E. coli, express it at an extremely high level, and produce your protein of interest.

Thus µSCALE empties the contents of a single capillary onto a collector plate, where the DNA of the isolated cell can be sequenced and the gene variant responsible for the protein of interest can be identified.

Antibodies are a go - to tool for detecting a protein of interest in cells and tissues.

To find coworkers of a protein of interest, Mayer and her colleagues build a tiny molecule called a tag into the initial protein.

The tag in their unique method is made up of a «tetracysteine motif» — two pairs of the amino acid cysteine separated by two other amino acids that doesn't interfere with the normal function of the protein of interest.

Construction of genetic models continues to be a most effective measure in understanding gene functions because loss of functional mutants accurately reflects non-redundant protein functions and elucidates the cellular impact of a protein of interest.

Such assays enable researchers to identify small molecule compounds which interact with a target protein of interest.

An assay developed in our lab uses a reference protein that is translated from the same open - reading frame and separated from the protein of interest by a 2A self - cleaving peptide (Goedhart et al, 2010).

- Based on the expression level / pattern of the protein of interest, decide what antigen to detect with which fluorophore in multi-color experiments.

Moderna's mRNA Therapeutics ™ platform builds on the discovery that modified mRNA can direct the body's cellular machinery to produce nearly any protein of interest, from native proteins to antibodies and other entirely novel protein constructs with therapeutic activity inside and outside of cells.

We recommend the full - length fusion protein procedure if your protein of interest has low sequence similarity with other proteins.

Just pull down your protein of interest with immobilized nanobodies, also termed VHHs or single domain antibodies.

developed in our lab uses a reference protein that is translated from the same open - reading frame and separated from the protein of interest by a

To make sure they are getting data only on the protein of interest, the researchers label their target protein with a radioactive form of carbon known as carbon - 13, while the rest of the proteins are unlabeled.

Then, massively parallel sequencing yields all of the RNA «tags» bound by the protein of interest.

FACS - if you're knocking out a cell surface protein, then cell sorting can be used to rapidly isolate those clones that have lost the protein of interest

The researchers have narrowed their list from more than 2,000 proteins of interest to just 200 promising candidates.

Biochemists and molecular biologists who need to overexpress and purify proteins can face any number of technical challenges depending on their protein of interest.

To better understand a protein's function, researchers often use a technique called x-ray crystallography to determine the three - dimensional architecture of the protein of interest.

Furthermore, the DT40 platform is not restricted to FPs alone as protein evolution can be achieved for any protein of interest whereby a suitable selection scheme can be implemented [11].

It involves adjusting the pH of a solution, so the proteins of interest have a net positive or negative charge.