Sentences with phrase «only cell cultures»

Up to now, only cell cultures gave an indication of this astonishing ability of the brain.

Not exact matches

[Perhaps the concept of «church - court» has moved to the openness of the Internet, and we all are now part of the greater «church court»... but then again, I can only wonder how to implement the third step within a global culture interconnected by the Internet, cell phones, tweets, and videos....

Still, only a few dozen labs in the world are certified as BSL4 facilities; some are very small and only work on diagnostics, or cell cultures, or a single species of animals.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

Dr. Zubair already grows such cells in his Mayo Clinic laboratory using a large tissue culture and several incubators — but only at a snail's pace.

Upon expression in cultured cells, the new subunits yielded prominent, typical glutamate - and NMDA - activated currents only when they were in heteromeric configurations with NR1.

In rats and tissue cultures of human nerve cells, these «beta sheet breakers» not only prevent amyloid plaques from forming, but also dissolve existing plaques.

During her postdoctoral work, Bissell noticed that removing mammary cells from a mouse and putting them into a culture dish caused them to lose not only their normal in vivo shapes, but also their ability to secrete milk.

They found that when IGF - 1 was added to the cultured blood cells there was increased lithium sensitivity only in the blood cells of those bipolar disorder patients who did not respond to lithium therapy.

When they mixed the two types of cells in a culture, only one type of cell mingled.

Though Li's adhesive has been tested only in human cell cultures and in lab animals, another bio-inspired glue has made its way into human trials.

«This pilot study showed that the culture system not only allowed for efficient propagation of sperm stem cells, but also eliminated ALL cells,» said Sadri - Ardekani.

But boys who haven't been through puberty can only have sperm stem cells removed and frozen in anticipation of technology that could culture the cells and place them back in the testes, where they produce sperm after puberty.

Charge - holding capacity was only marginally reduced for the saline - and cell - culture - based batteries, most likely because they had slightly lower sodium - ion content than the sodium sulfate solution.

The research highlights the importance of studying the effects of drugs not only on the tumor cells in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.

In other words, a culture with a million healthy cells and one p53 mutant cell, said Eggan, could quickly become a culture of only mutant cells.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

Many researchers have tried to culture kidney progenitor cells, but these cells could only be kept for two to three days at most.

But one stem - cell culture showed a dramatic change: as the cells grew and divided, levels of the carried - over mtDNA jumped from 1.3 % to 53.2 %, only to later plummet down to 1 %.

Normal cells often have proved impractical because they can only divide a limited number of times in culture, and once returned to the body they're often too old to do much good.

When Patapoutian and his colleagues added each sulfur compound individually to cultured cells, only allicin triggered a fast, intense response like the raw extract did.

Only in 2004 did scientists finally succeed in growing such cells in culture from mice.

The cultures can be kept alive for different periods of time — 14 to 20 days at most — and not only is it possible to examine the ultrastructures of the cells, but also the effects of different agents on those structures.

Zheng explained that in the past, measuring NMD efficiency was a cumbersome process and could be performed only on cell cultures, not directly on tissues.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

Control ganglia cultured for the same length of time in the absence of the sarcoma - 180 cells displayed only a sparse and irregular outgrowth of nerve fibers.

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

So why, Taylor proposes, limit a stem cell implantation to only one kind of cultured, differentiated cell?

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

We then prepared cultures from this tissue, but we observed only very few GFAP or S100β positive astrocytes, while the majority of cells were negative for all glial and neuron markers tested, suggesting that these cells may be non-neural.

The CRISPR / Cas system is amazingly versatile and has proven to edit the genome effectively not only in mice and flies, but also in monkeys and, yes, in human cultured cells.

High mRNA expression levels of HNF4α and ASGPR1 are shown in the enhanced hiPS - HEP cells for an extended culture time and are comparable to levels in cryopreserved human primary hepatocytes (hphep cells) that have been grown for only a short time.

Notably, both in hphep cells and enhanced hiPS - HEP cultures, only a subset of hepatocytes is strongly stained for glycogen storage — again in agreement with the metabolic zonation observed in the liver lobe (Figure 3, Panel A).

Notably, both in hphep cells and the enhanced hiPS - HEP cultures, only a subset of hepatocytes is strongly stained for glycogen storage (shown by Periodic acid - Schiff staining, below)-- again in agreement with the metabolic zonation observed in the liver lobe.

While learning how to culture rhinovirus C, Palmenberg's research team discovered the virus only infects certain lung cells that express a particular genetic form of a cell - surface molecule called cadherin - related protein 3 (CDHR3).

In these tests, StemFit not only delivered higher cell proliferation, but also showed characteristics such as homogeneity of gene expression compared with iPS cells cultured with four other media without any chromosomal abnormalities.

Immediately after the cells were seeded, each well was checked under the microscope to verify the sparseness of each spherical culture, and only wells containing single - cell suspension with no cell cluster were chosen for tumor - sphere counting after 9 days.

As bioinformatics approaches can only provide partial solutions, a functional analysis pipeline, based on primary (fibroblast) cell cultures, induced Pluripotent Stem cells (iPS) or simple model organisms, is truly essential.

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

Furthermore, the proliferation - inactivated MEF cells usually function well only for 5 — 7 days under ES or iPS cell culture conditions.

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

Taken together our data support a model in which ES cell culture has trapped a set of interconvertible cell states reminiscent of the early stages in blastocyst differentiation that may exist only transiently in the early embryo.

The existence of a paracrine inter-dependent equilibrium would suggest that the culture conditions have selected for the stable coexistence of mutually dependent and metastable cell types that only transiently exist in vivo.

Scientists have so far only achieved success in cell cultures and in mice.

Pioneering work by Barrandon and Green more than 20 years ago (2) showed that when primary human keratinocytes were put in culture, their abilities to establish a clone were related to the heterogeneity in cell size — only cells ≤ 11 μm in diameter could form a clone whereas cells ≥ 12 μm were irreversibly committed to terminal differentiation.

Recruiting Volker was one of the biggest strokes of luck in my scientific life as he was not only the key person to set up the first HCV cell culture system, but also an exceptional mind creating true team spirit and synergy within the research team.

I am really looking forward to this paper I believe up until now it has been proved only in rodents or cell cultures — right?

This is the process, tested so far only at laboratory scale, of growing pork, chicken, or beef through cell culture in vats instead of raising and slaughtering animals.