Now, if you have any questions about the bacterial
arabinose operon, we've got your guy.»
Expression of the Escherichia coli
tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical.
As a grad student, I once spent 2 months demonstrating that three particular amino acids on a protein in the
arabinose operon (the group of genes that allows bacteria to metabolize a certain sugar) do not play a role in causing that protein to bind to other copies of itself.
In bacteria, the expression of genes related to bioluminescence is controlled by
an operon called the Lux operon.
An operon is a cluster of structural genes found in bacteria that are expressed and regulated as a unit.
To deduce the role of the Pap
operon proteins on cellular function, the researchers performed a «knockout» of the entire operon.
The operator is a segment of DNA that regulates the activity of the structural genes of
the operon, somewhat like an on / off switch.
It consists of a promoter and an operator; a promoter is the region of
the operon that acts as an initial binding site during gene transcription.
In E. coli, the four ribosomal proteins of the alpha
operon are translationally repressed by ribosomal protein S4, one of the proteins encoded in the operon.
WH8102 and B. burgdorferi, rpsD, encoding S4, maps outside of
the operon, suggesting a different mechanism of regulation.