Sentences with word «organotypic»

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

We have recently proposed, from experiments in organotypic cultures, that specific central pattern generator networks, upstream of magnocellular neurons, determine their bursting activity.

In addition, melanocytes integrated appropriately in organotypic epidermis reconstructed in vitro.

The unique organotypic models developed for this study also hold promise for future therapy research.

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

An ex vivo organotypic culture system was used to examined exposure effects at the time of fetal testosterone production initiation at GD14.5.

To this aim we have screened our electrophysiological recordings of the magnocellular neurons, previously obtained from acute slices, with an analysis of autocorrelation of action potentials to detect a rhythmic drive as we recently did for organotypic cultures.

The Tübingen group was making organotypic eye cultures and examining in vitro the changes that occur in the cells of living individuals.

In the Finkbeiner laboratory, Linsley uses time - lapse imaging of neurons in culture, within organotypic mouse brain slices, and within live zebrafish to uncover the mechanisms in neurodegeneration that lead to neuronal death.

Flavivirus infection of Ixodes scapularis (black - legged tick) ex vivo organotypic cultures and application for control.

Long - term goal of the project will be to visualize and manipulate tau expression also in a systemic context (organotypic slice preparations, mouse brain) by affecting the function of tau mRNA - containing neuronal granules as a potential drug target for the treatment of neurodegenerative diseases.

In vitro studies include single molecule measurements of polarity complexes using TIRF microscopy and CRISPR gene - editing; genome - wide CRISPR screens for genes that regulate homeostais; 3D culture of mammary gland organoids; human breast tissue organotypic cultures; and renal epithelial cysts.

With their organotypic models, Ghajar, Bissell and their collaborators discovered that the protein thrombospondin - 1, which is prevalent in stable microvasculature, creates a dormant niche by suppressing the growth of breast cancer cells.

«Our organotypic models demonstrate the power of the microenvironment by showing that normal cells in their native architecture naturally steer fully malignant, genotypically aberrant tumor cells into a quiescent state,» Ghajar says.

We use cell and molecular biology, mouse models of cancer, and organotypic model systems to address questions in cancer biology, immunology, and hemostasis.

Two exogenous murine neural stem cells (NSCs) filled with the gap junction - permeable dye Calcein (green) and the gap junction - impermeable dye DiI (red) following engraftment to an organotypic stritatal tissue culture.

The cellular mechanisms underlying this bursting behavior have been studied in vitro, using either acute slices of the adult hypothalamus, or organotypic cultures of neonatal hypothalamic tissue.

«In the future, organotypic models that reflect the unique biology of individual patients could be used in screening.