To determine whether the reactivation of methylation - silenced genes by EGCG is a general phenomenon that also occurs
in other cell lines, we studied the effects of EGCG treatment on the methylation status and mRNA levels of p16INK4a or RARβ in three other human cancer cell lines (Fig. 3C) ⇓.
Their method, called PRISM, uses a molecular barcoding system to test potential drug compounds on cancer and
other cell lines at an unprecedented scale and speed.
Often it is more spherically shaped and centered around the microtubule organizing center, whereas it encircles the whole nucleus
in other cell lines.
But we had
no other cell line to go with, so we all agreed that we'd use these mammary - gland cells and just see what happened, gain some experience.
Researchers have conducted so many studies with HeLa cells that they've become somewhat of a problem; scientists will periodically suffer the embarrassment of finding that HeLa cells contaminate
their other cell lines.
Opening new doors for the study of this poorly characterized subset of the proteome, the researchers» method is applicable to
other cell lines.
Mycoplasma contamination has been shown to arise from a variety of sources such as serum,
other cell lines, or infected personnel and can persist undetected; unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy.
Strengthening and guarding gut and
other cell linings, only allowing molecules they recognize to get through.