Not exact matches
Building from two subunits, alpha and beta
tubulin, this protein assembles into microtubules that play a vital role inside cells — giving structure, pushing or pulling
other things around, or providing a track on which
other molecules can pull themselves along.
One such compound was docetaxel, an anticancer drug that binds
tubulin, and the
other jasplakinolide, which specifically binds the cytoskeletal form of actin.
One reaction serves as a positive control to amplify a portion of the
tubulin gene found in all plants, and the
other assays for genetic modification.
Interference with a host of cellular functions tested and synergistic activities with a wide range of
other drugs is best explained by direct interaction with various DNA sequences and general interference with replication and transcription, except for some effects exerted by binding to
tubulin.
The labelling is for acetylated
tubulin in red (identifying all axons), and green for the cell permeable dye calcein, which is only applied on the axonal side of the chambers (top half) and allows the identification of those neuronal cell bodies (bottom half) that have extended axons to the
other side of the microfluidic device.
Other tissues are stained with anti-acetylated
tubulin (coloured green).