Proco recently installed its all new advanced dual Robot
packaging cell for Healthcare industry.
As a test of this idea, two retroviral vectors — one encoding a hammer - head ribozyme designed to cleave lacZ transcripts and another encoding the lacZ messenger RNA — were coexpressed inside
retroviral packaging cells.
Briefly,
293FT packaging cells were transfected with pLL3.7 - GFP lentiviral vector, together with the packaging plasmids, using Fugene 6.
A successful lentiviral infection begins with your cells, both
your packaging cells and your target cells.
Human embryonic kidney (HEK) 293T cells,
a packaging cell line for lentivirus production, and the preosteoclast cell line RAW264.7 (ATCC) were maintained in DMEM supplemented with 10 % FBS and antibiotics.