Sentences with phrase «paired zinc finger»

Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove exon 51 in DMD patient myoblasts.

Not exact matches

DNA polymorphism in the Y chromosome, examined at a 729 - base pair intron located immediately upstream of the ZFY zinc - finger exon, revealed no sequence variation in a worldwide sample of 38 human males.

To genetically disrupt the CXCR4 allele, we designed a pair of zinc - finger proteins (ZFPs) targeting the region of the cxcr4 gene that encodes residues Asp 187 to Val 196 in the second extracellular loop (ECL2) of this seven - transmembrane domain receptor using methods previously described [29]--[32](Figure 1).

(A) A CXCR4 - specific ZFN pair was generated, comprised of two DNA - binding zinc finger proteins (ZFPs) each fused with a FokI endonuclease monomer.

Here we describe engineering a pair of zinc finger nucleases that, when introduced into human T cells, efficiently disrupt cxcr4 by cleavage and error - prone non-homologous DNA end - joining.

To circumvent this, a CCR5 specific zinc - finger nuclease pair (R5 - ZFNs) has been developed [23].